UPLC-MS/MS method for simultaneous determination of cyclophosphamide, docetaxel, doxorubicin and 5-fluorouracil in surface samples

Silva, Carla Brigagão Pacheco da; Julio, Isabelle Prado; Donadel, Giuliano Engler; Martins, Ignez Salas

doi:10.1016/j.vascn.2016.08.004

Cited by 27 publications

(25 citation statements)

References 13 publications

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“…The development of chromatographic methods for simultaneous analysis of polar and non-polar compounds has piqued the interest of the international scientific community (38), including our research group. More specifically, some authors (13)(14)(15)21) have proposed LC methods for 5-FU resolution via a C18 stationary phase combining hydrophobic and polar selectivity. Since our goal was to analyse as many ADs as possible at the same time, given that cancer treatments often include more than one cytotoxic drug and that this increases the probability of having at least one positive finding, we preferred to use two LC columns: one for hydrophobic cytostatic drugs and one for the hydrophilic ones.…”

Section: Discussionmentioning

confidence: 99%

“…In the case of cytotoxic ADs, monitoring surface contamination in hospital environments usually involves wipe tests as a simple way to indirectly assess dermal occupational exposure (12). However, most of the monitoring (13)(14)(15)(16)(17)(18)(19)(20) is focused on no more than five ADs among the following drugs: CP, MT, iphosfamide (IP), paclitaxel (PTX), doxorubicin (DXR), 5-fluorouracyl (5-FU), docetaxel (DTX), epirubicin (EPI), and gemcitabine (GEM). Only a few (21)(22)(23) have expanded the range from seven to ten [5-FU, PTX, CP, IP, MT, GEM, DXR, EPI, vincristine (VNC), vinblastine (VNB), docetaxel (DTX), etoposide (ETP), cytarabine (CTB), irinotecan (IRT), oxaliplatin, and vindesine].…”

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confidence: 99%

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A new approach to assessing occupational exposure to antineoplastic drugs in hospital environments

Dugheri

Bonari

Pompilio

et al. 2018

Archives of Industrial Hygiene and Toxicology

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Cytotoxic antineoplastic drugs (ADs) pose occupational risk and therefore require safe handling practices. We created, optimised, and validated an innovative monitoring protocol for simultaneously assessing 21 ADs in the healthcare environment, and also proposed surface exposure levels (SELs) to facilitate the interpretation of monitoring results, as there are currently no occupational exposure limits for ADs. The environmental AD monitoring data were collected in nine Italian hospitals between 2008 and 2017 and include 74,565 measurements in 4,814 wipe samples. Excellent overall recovery and sensitivity of the analytical methods along with innovative desorption automation make this protocol useful for routine monitoring. AD contamination was found in 3,081 measurements, confirming potential exposure in healthcare workers. Samples taken at the beginning and the end of work shifts, allowed to calculate 75th and 90th percentile values for each ADs both in preparation and administration units and we created a traffic-light colour-coding system to facilitate interpretation of the findings. The introduction of SELs will provide a solid basis for improving occupational safety and focusing on contamination control.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

A new approach to assessing occupational exposure to antineoplastic drugs in hospital environments

Dugheri

Bonari

Pompilio

et al. 2018

Archives of Industrial Hygiene and Toxicology

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“…Particularly, the chromatography of 5-FU on surface samples, of which more than 400 kg are being administered in Italy each year and 8 t in Germany, has aroused interest in the scientific community. Some authors [23,30,31] proposed LC-MS/MS methods for efficient 5-FU resolution by using Phenomenex Kinetex Biphenyl, Agilent Pursuit XRs Ultra, or Shimadzu SHIM-PACK XR-ODC C18 columns.…”

Section: Discussionmentioning

confidence: 99%

Analytical strategies for assessing occupational exposure to antineoplastic drugs in healthcare workplaces

Dugheri¹,

Bonari²,

Pompilio³

et al. 2018

Med Pr

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Background: Cytotoxic antineoplastic drugs (ADs), widely used in treating cancer, are considered hazardous in the workplace and thus require safe handling practices. An analytical protocol for environmental and biological AD monitoring in the healthcare environment has been developed, since Europe lacks clear guidelines and regulations for cytostatic preparation and handling. Material and Methods: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used for measuring contemporaneously 20 multi-class cytostatic compounds and urinary α-fluoro-β-alanine, whereas platinum was detected by inductively coupled plasma mass spectrometry (ICP-MS). Sampling procedures and analytical conditions were optimized and the assays were validated. Environmental AD monitoring data, collected in 2009-2017, for a total of 3749 wipe tests and 57 720 determinations, was evaluated. Results: The proportion of positive samples gradually decreased from 11.7% in 2010 to 1% in 2017, however, 2266 determinations were positive. No urine sample had detectable concentrations of any of the 4 drugs considered (0/398 samples). Conclusions: These improvements are so large that the key role played by the new, more stringent rules for preparing and administering ADs is evident. Hence, the analytical method involving multi-element determinations allows for a more thorough and complete investigation into the AD contamination of work environments.

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“…In this same line, da Silva et al [71] developed an UPLC-MS/ MS method for the simultaneous determination of DTX, cyclophosphamide, doxorubicin, and 5-fluorouracil in order to evaluate the contact of workers exposed to these antineoplastic drugs. Chromatographic separation was done using a SHIM-PACK XR-ODC-C 18 column (100 mm £ 3.0 mm i.d.…”

Section: Methodsmentioning

confidence: 99%

A Critical Review of Properties and Analytical Methods for the Determination of Docetaxel in Biological and Pharmaceutical Matrices

Hanck-Silva

Fernandes

Trevizan

et al. 2018

Critical Reviews in Analytical Chemistry

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Docetaxel (DTX) is an antineoplastic agent of the second generation of the taxoid family. It is a semi-synthetic drug prepared from a precursor extracted of the plant Taxus baccata. The commercial formulation of DTX, Taxotere®, employs the surfactant polysorbate 80, due to the low water solubility of the drug, causing several side effects. Therefore, there is a need to develop delivery systems to reduce the side effects of DTX. In addition, this drug has been qualitative and quantitatively analyzed in pharmaceutical formulations and biological samples. Thus, several techniques and analytical methods have been reported with the aim of optimizing the analytical signal, increasing sensitivity, selectivity and reducing the effects of interference. Herein, we highlight immunoassay, capillary electrophoresis and chromatographic methods. This review presents a summary of physicochemical and pharmacokinetics properties, mechanisms of action, drug delivery systems and analytical methods used in quantification of DTX in diverse matrices such as blood, plasma, oral fluid, urine, carcinoma cells, pharmaceutical formulations and delivery systems.

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UPLC-MS/MS method for simultaneous determination of cyclophosphamide, docetaxel, doxorubicin and 5-fluorouracil in surface samples

Cited by 27 publications

References 13 publications

A new approach to assessing occupational exposure to antineoplastic drugs in hospital environments

A new approach to assessing occupational exposure to antineoplastic drugs in hospital environments

Analytical strategies for assessing occupational exposure to antineoplastic drugs in healthcare workplaces

A Critical Review of Properties and Analytical Methods for the Determination of Docetaxel in Biological and Pharmaceutical Matrices

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