UPLC-MS/MS Method for Analysis of Endocannabinoid and Related Lipid Metabolism in Mouse Mucosal Tissue

Wiley, Mark B; Perez, Pedro A.; Argueta, Donovan A; Avalos, Bryant; Wood, Courtney; DiPatrizio, Nicholas V.

doi:10.3389/fphys.2021.699712

Cited by 9 publications

(5 citation statements)

References 45 publications

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“…The pretreated data were uploaded into SIMCA-P (version 14.1, Umetrics, Umea, Sweden) for multivariate data analysis (Wiley et al, 2021). Orthogonal partial least-squares discriminant analysis (OPLS-DA) is a statistical method of supervised discriminant analysis.…”

Section: Discussionmentioning

confidence: 99%

Multi metabolomics-based analysis of application of Astragalus membranaceus in the treatment of hyperuricemia

2022

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Hyperuricemia (HUA) is a common metabolic disease that is an independent risk factor for comorbidities such as hypertension, chronic kidney disease, and coronary artery disease. The prevalence of HUA has increased over the last several decades with improved living standards and increased lifespans. Metabolites are considered the most direct reflection of individual physiological and pathological conditions, and represent attractive candidates to provide deep insights into disease phenotypes. Metabolomics, a technique used to profile metabolites in biofluids and tissues, is a powerful tool for identification of novel biomarkers, and can be used to provide valuable insights into the etiopathogenesis of metabolic diseases and to evaluate the efficacy of drugs. In this study, multi metabolomics-based analysis of the blood, urine, and feces of rats with HUA showed that HUA significantly altered metabolite profiles. Astragalus membranaceus (AM) and benbromomalone significantly mitigated these changes in blood and feces, but not in urine. Some crucial metabolic pathways including lipid metabolism, lipid signaling, hormones synthesis, unsaturated fatty acid (UFAs) absorption, and tryptophan metabolism, were seriously disrupted in HUA rats. In addition, AM administration exerted better treatment effects on HUA than benbromomalone. Furthermore, additional supplementation with UFAs and tryptophan may also induce therapeutic effects against HUA.

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Section: Discussionmentioning

confidence: 99%

Multi metabolomics-based analysis of application of Astragalus membranaceus in the treatment of hyperuricemia

2022

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“…Mobile phases consisted of A (1 mL formic acid and 3.385 g ammonium acetate per liter) and B (0.1% formic acid). [ 23 ] Besides, targeted LC‐MS/MS was conducted to further identify the differential metabolites.…”

Section: Methodsmentioning

confidence: 99%

Docosahexaenoic Acid Modulates Nonalcoholic Fatty Liver Disease by Suppressing Endocannabinoid System

Chen,

Fan,

Wang

et al. 2024

Molecular Nutrition Food Res

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ScopeEndocannabinoid signaling regulates energy homeostasis, and is tightly associated with nonalcoholic fatty liver disease (NAFLD). The study previously finds that supplementation of docosahexaenoic acid (DHA) has superior function to ameliorate NAFLD compared with eicosapentaenoic acid (EPA), however, the underlying mechanism remains elusive. The present study aims to investigate whether DHA intervention alleviates NAFLD via endocannabinoid system.Methods and resultsIn a case–control study, the serum endocannabinoid ligands in 60 NAFLD and 60 healthy subjects are measured. Meanwhile, NAFLD model is established in mice fed a high‐fat and ‐cholesterol diet (HFD) for 9 weeks. DHA or EPA is administrated for additional 9 weeks. Serum primary endocannabinoid ligands, namely anandamide (AEA) and 2‐arachidoniylglycerol (2‐AG), are significantly higher in individuals with NAFLD compared with healthy controls. NAFLD model shows that serum 2‐AG concentrations and adipocyte cannabinoid receptor 1 expression levels are significantly lower in DHA group compared with HFD group. Lipidomic and targeted ceramide analyses further confirm that endocannabinoid signaling inhibition has exerted deletion of hepatic C16:0‐ceramide contents, resulting in down‐regulation of de novo fatty acid synthesis and up‐regulation of fatty acid β‐oxidation related protein expression levels.ConclusionsThis work elucidates that DHA has improved NAFLD by suppressing endocannabinoid system.

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“…Spectrometry. To understand the changes in metabolites and metabolic networks in myocarditis after antitumor immunotherapy, we investigated metabolomic differences between myocarditis samples by ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/ MS) in combination with database alignment and multivariate bioinformatics analysis [20,21]. Samples were collected through myocardial biopsies from three patients who were diagnosed by experienced cardiovascular specialists with myocarditis after targeted immunotherapy for gastrointestinal tumors from December 1, 2020 to December 31, 2021.…”

Section: Sample Preparation For Chromatography-massmentioning

confidence: 99%

The Regulation Network of Glycerolipid Metabolism as Coregulators of Immunotherapy-Related Myocarditis

Yang

Duan

Xia

et al. 2023

Cardiovascular Therapeutics

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Background. To date, immunotherapy for patients with malignant tumors has shown a significant association with myocarditis. However, the mechanism of metabolic reprogramming changes for immunotherapy-related cardiotoxicity is still not well understood. Methods. The CD45+ single-cell RNA sequencing (scRNA-seq) of the Pdcd1-/-Ctla4+/- and wild-type mouse heart in GSE213486 was downloaded to demonstrate the heterogeneity of immunocyte atlas in immunotherapy-related myocarditis. The liquid chromatography–tandem mass spectrometry (LC-MS/MS) spectrum metabolomics analysis detects the metabolic network differences. The drug prediction, organelle level interaction, mitochondrial level regulatory network, and phosphorylation site prediction for key regulators have also been screened via multibioinformatics analysis methods. Results. The scRNA analysis shows that the T cell is the main regulatory cell subpopulation in the pathological progress of immunotherapy-related myocarditis. Mitochondrial regulation pathway significantly participated in pseudotime trajectory- (PTT-) related differential expressed genes (DEGs) in the T cell subpopulation. Additionally, both the gene set enrichment analysis (GSEA) of PTT-related DEGs and LC-MS/MS metabolomics analysis showed that mitochondrial-regulated glycerolipid metabolism plays a central role in metabolic reprogramming changes for immunotherapy-related cardiotoxicity. Finally, the hub-regulated protease of diacylglycerol kinase zeta (Dgkz) was significantly identified and widely played various roles in glycerolipid metabolism, oxidative phosphorylation, and lipid kinase activation. Conclusion. Mitochondrial-regulated glycerolipid metabolism, especially the DGKZ protein, plays a key role in the metabolic reprogramming of immunotherapy-related myocarditis.

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UPLC-MS/MS Method for Analysis of Endocannabinoid and Related Lipid Metabolism in Mouse Mucosal Tissue

Cited by 9 publications

References 45 publications

Multi metabolomics-based analysis of application of Astragalus membranaceus in the treatment of hyperuricemia

Multi metabolomics-based analysis of application of Astragalus membranaceus in the treatment of hyperuricemia

Docosahexaenoic Acid Modulates Nonalcoholic Fatty Liver Disease by Suppressing Endocannabinoid System

The Regulation Network of Glycerolipid Metabolism as Coregulators of Immunotherapy-Related Myocarditis

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