UPLC-MS/MS determination of steroid hormones via a novel reaction based on derivatisation by a cyclic-organophosphate

Szterk, Arkadiusz; Sitkowski, Jerzy; Bocian, Wojciech

doi:10.1016/j.talanta.2019.06.046

Cited by 3 publications

(1 citation statement)

References 36 publications

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“…Although some authors add a derivatization step to the whole procedure to minimize the matrix effect and increase the sensitivity of the method, ,− we did not consider this step in our study, because our goal of determining simultaneously several steroid hormone classes is not compatible with the application of a single derivatization procedure.…”

Section: Resultsmentioning

confidence: 99%

Analytical Strategy for Identification and Quantification of 13 Steroids in Sole (Solea senegalensis) Tissues, Eggs, and Larvae for Application in Aquaculture Studies of Reproduction

Serrano¹,

Beltrán²,

Piquer

et al. 2021

ACS Agric. Sci. Technol.

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Knowing the levels of steroids in fish is crucial to understand the reproductive processes during fish development and for reproductive success in aquaculture facilities. Although some of these compounds are present at very low concentrations, they play a relevant role in reproduction processes. Therefore, very sensitive and reliable analytical methodology is required for their accurate determination. In this work, ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) with triple quadrupole has been optimized for the identification and quantification of 13 steroids in plasma, muscle and ovaries of sole (Solea senegalensis) and, for the first time, in fish eggs and larvae. This is noteworthy because studying fish development in early stages could be the key to solving the reproductive problems observed in farmed specimens. Different strategies have been applied to solve analytical challenges such as matrix effects or the small amount of sample available. Compounds selected include progestagens (progesterone, 17α,20β-dihydroxypregnenone, 17α,20β,21trihydroxypregnenone), androgens (androstenedione, 11β-hydroxiandrostenedione, 11ketoandrostenedione, testosterone, 5α-dihydrotestosterone, 11-ketotestosterone), estrogens (estrone, estradiol), and corticosteroids (cortisol, 11-deoxycortisol). The methodology was validated in terms of accuracy (between 73-131%) and precision (relative standard deviations below 24%), at concentrations as low as 0.1 ng/mL for plasma, 0.1 ng/g for muscle and eggs, and 0.5 ng/g for ovaries and larvae. The application of the developed methodology to the analysis of samples from captive fish has allowed to identify and quantify steroids at sub-ng/mL or sub-ng/g.

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