UPLC-MS-ESI-QTOF characterization and evaluation of the antibacterial and modulatory antibiotic activity of Ziziphus joazeiro Mart. aqueous extracts

Andrade, Jacqueline Cosmo; Silva, A.R.P.; Santos, Antônia Thassya Lucas dos; Freitas, Maria Audilene de; Carneiro, Joara Nályda Pereira; Gonçalo, Maria Isabeli Pereira; Souza, Aca; Freitas, Thiago Sampaio de; Ribeiro, Paulo Riceli Vasconcelos; Brito, Edy Sousa de; Morais-Braga, Maria F. B.; Coutinho, Henrique Douglas Melo

doi:10.1016/j.sajb.2019.02.001

Cited by 25 publications

(11 citation statements)

References 24 publications

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“…In the leaf extracts (L1-L8), it was possible to observe the presence of signals between 0 and 30 min (region I) in both the TICs and DAD chromatograms, indicating that these samples possessed substances with chromophores at the four selected wavelengths. This is in accordance with the recent work from Andrade et al (2019), which reported the presence of catechin and flavonoid glycosides in the aqueous extracts of the leaves, but not in aqueous extracts of the stem bark of this plant. In the region between 45 and 58 min (region II), only peaks in the TIC were observed.…”

Section: Lc-dad-mssupporting

confidence: 93%

Ziziphus joazeiro, a Saponin-Rich Brazilian Medicinal Plant: Pharmacognostic Characterization of Bark and Leaves

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Fonseca

Campos

et al. 2020

Rev. Bras. Farmacogn.

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Ziziphus joazeiro Mart., Rhamnaceae, is a plant native to northeast Brazil where it is known as "juazeiro." Its bark, rich in saponins, is widely utilized in folk medicine and as a supply to produce cosmetics. Aiming at the preservation of the species and the search for an alternative vegetal raw material, the phytochemical profiles of bark and leaves were compared by chemical techniques. The foaming index and gravimetric studies indicated that, in general, bark values were approximately twice than those of the leaves. The same occurred with the hemolysis indexes. Extracts were dereplicated by liquid chromatography with diode array detection coupled to mass spectrometry analyses using an atmospheric pressure chemical ionization source, which indicated that at least 5 saponins were common to all bark and leaf samples. The direct infusion high-resolution mass spectrometry analyses revealed at least 44 different saponins distributed between the bark and leaves of the analyzed plant, of which 40 were described for the first time. The data showed that there were similarities between the studied plant organs as demonstrated by mass spectrometry, foaming index, and hemolysis indexes. Therefore, with the application of a suitable standardization procedure the leaves could be a promising source for obtaining "juazeiro-type" saponins.

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Section: Lc-dad-mssupporting

confidence: 93%

Ziziphus joazeiro, a Saponin-Rich Brazilian Medicinal Plant: Pharmacognostic Characterization of Bark and Leaves

Nascimento

Fonseca

Campos

et al. 2020

Rev. Bras. Farmacogn.

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“…Bark extract of Ziziphus joazeiro Mart., Which composition had numerous flavonoid compounds, was tested against 3 g-positive strains ( Staphylococcus aureus , Staphylococcus epidermidis and Enterococcus faecalis ), presenting 8 antagonistic results out of 9 possible [ 8 ]. On the other hand, a study with isolated flavonoids derived from plants demonstrated a high modulatory capacity on the S. aureus strain.…”

Section: Resultsmentioning

confidence: 99%

“…Based in this situation, the studies seeking secondary metabollites from plants to combat the bacterial resistance to antibiotics as na adjuvante or as na antibacterial agente with direct action are more and more importante [ [5] , [6] , [7] , [8] , [9] , [10] ]. Therefore, the use of medicinal plants as a source of these new alternatives is emphasized, which may be a promising approach [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of phytochemical composition, toxicity in Drosophila melanogaster and effects on antibiotics modulation of Plathymenia reticulata Benth extract

et al. 2021

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“…The chemical composition of the extract was determined using the Acquity UPLC system (Waters Corporation, Milford, MA, USA) coupled with a Quadrupole/Time of Flight system (QTOF). Chromatographic runs were performed on a Waters Acquity UPLC BEH column (150 × 2.1 mm, 1.7 µm) injected with 5 µL of the extract solution, with the temperature adjusted to 40 • C. The binary gradient elution system consisted of 0.1% formic acid in water (A) and 0.1% formic acid in acetonitrile (B), with a linear gradient from 2% to 95% of B (0-15) min) and a flow rate of 0.3 mL/min [80].…”

Section: Phytochemical Analysis 431 Qualitative Analysismentioning

confidence: 99%

Chemical Constituents and Biological Activities of Croton heliotropiifolius Kunth

et al. 2021

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Croton heliotropiifolius Kunth (Euphorbiaceae), whose occurrence has already been registered in the most varied Brazilian biomes, is commonly found in the Chapada do Araripe, Ceará. The species is traditionally used to treat fungal, parasitic, and degenerative diseases. This study investigated the chemical composition and pharmacological potential (antioxidant, antifungal, antiparasitic, and cytotoxic) of an aqueous extract obtained from the roots of C. heliotropiifolius. Following a qualitative phytochemical screening, the chemical constituents were identified by ultra-efficiency liquid chromatography coupled witha quadrupole/time-of-flight system (UPLC-QTOF). The antioxidant potential was verified by thin-layer chromatography (TLC). The direct and combined antifungal activity of the extract against opportunistic Candida strains was investigated using the microdilution method. The minimal fungicidal concentration (MFC) was determined by subculture, while the modulation of the morphological transition (fungal virulence) was evaluated by light microscopy. The in vitro antiparasitic activity was analyzed using epimastigotes of Trypanosoma cruzi and promastigotes of Leishmania braziliensis and Leishmania infantum, while cytotoxicity was determined in cultures of mouse fibroblasts. The phytochemical analysis identified the presence of acids, terpenes, flavonoids, lignans, and alkaloids. Among these constituents, the presence of polar and non-polar phenolic compounds with known antioxidant action washighlighted. While the extract showed clinically ineffective antifungal effects, it could enhance the effectiveness of fluconazole, in addition to inhibiting the morphological transition associated with increased virulence in Candida strains. Although the extract showed low cytotoxicity against fibroblasts, it also had weak antiparasitic effects. In conclusion, Croton heliotropiifolius is a source of natural products with antifungal and antioxidant potential.

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UPLC-MS-ESI-QTOF characterization and evaluation of the antibacterial and modulatory antibiotic activity of Ziziphus joazeiro Mart. aqueous extracts

Cited by 25 publications

References 24 publications

Ziziphus joazeiro, a Saponin-Rich Brazilian Medicinal Plant: Pharmacognostic Characterization of Bark and Leaves

Ziziphus joazeiro, a Saponin-Rich Brazilian Medicinal Plant: Pharmacognostic Characterization of Bark and Leaves

Evaluation of phytochemical composition, toxicity in Drosophila melanogaster and effects on antibiotics modulation of Plathymenia reticulata Benth extract

Chemical Constituents and Biological Activities of Croton heliotropiifolius Kunth

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