UPLC–MS-Based Non-targeted Analysis of Endogenous Metabolite Changes in the Leaves of Scabiosa tschiliensis Grüning Induced by 6-Benzylaminopurine and Kinetin

Du, Jialin; Ma, Weiwei; Li, Yang; Lu, Xu; Geng, Zhaopeng; Huang, Hangjun; Yuan, Yuanyuan; Liu, Yue; Wang, Xiaodong; Wang, Junli

doi:10.3389/fpls.2021.700623

Cited by 7 publications

(5 citation statements)

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“…Kinetin affects metabolic pathways, especially the phenylpropanoid pathway which is a key piece of the shikimate pathway. The pathways play a crucial role in the biosynthesis of crucial precursors for a variety of secondary metabolites necessary for plant growth such as coumarins, flavonoids, and lignans [ 78 ]. The exogenous addition of kinetin in plants via foliar applications advances crop quality, yield growth rate, and tolerance to abiotic stresses.…”

Section: Discussionmentioning

confidence: 99%

Farm or Lab? A Comparative Study of Oregano’s Leaf and Callus Volatile Isolates Chemistry and Cytotoxicity

Kakalis

Tsekouras

Mavrikou

et al. 2023

Plants

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Oregano (Origanum vulgare, Lamiaceae plant family) is a well-known aromatic herb with great commercial value, thoroughly utilized by food and pharmaceutical industries. The present work regards the comparative assessment of in vitro propagated and commercially available oregano tissue natural products. This study includes their secondary metabolites’ biosynthesis, antioxidant properties, and anticancer activities. The optimization of callus induction from derived oregano leaf explants and excessive oxidative browning was performed using various plant growth regulators, light conditions, and antioxidant compounds. The determination of oregano callus volatiles against the respective molecules in maternal herbal material was performed using gas chromatography–mass spectrometry (GC/MS) analysis. In total, the presence of twenty-seven phytochemicals was revealed in both leaf and callus extracts, from which thirteen molecules were biosynthesized in both tissues studied, seven compounds were present only in callus extracts, and seven metabolites only in leaf extracts. Carvacrol and sabinene hydrate were the prevailing volatiles in all tissues exploited, along with alkanes octacosane and triacontane and the trimethylsilyl (TMS) derivative of carvacrol that were detected in significant amounts only in callus extracts. The MTT assay was employed to assess the in vitro cytotoxic properties of oregano extracts against the epithelial human breast cancer MDA-MB-231 and the human neuroblastoma SK-N-SH cell lines. The extracts displayed concentration and time-dependent responses in cell proliferation rates.

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Section: Discussionmentioning

confidence: 99%

Farm or Lab? A Comparative Study of Oregano’s Leaf and Callus Volatile Isolates Chemistry and Cytotoxicity

Kakalis

Tsekouras

Mavrikou

et al. 2023

Plants

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“…These cannabis alkaloids have demonstrated antiparasitic, antipyretic, antiemetic, antitumor, diuretic, and analgesic properties [ 11 , 71 ]. Neurine can be biosynthesized from choline [ 72 ], which has been classified as an essential nutrient for humans, and additionally, it is a precursor of the osmoprotectant glycine betaine, an enhancer of osmotic resistance in the plant against drought and salinity [ 73 ]. Choline biosynthesis is thus a potential nutraceutical pathway by which 3 methylation reactions occur, catalyzed in parallel by the cytosolic enzyme phosphoethanolamine N-methyltransferase (EC 2.1.1.103) and mediating the next 2 methylations to produce phosphocholine [ 73 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-Scale Metabolic Reconstruction, Non-Targeted LC-QTOF-MS Based Metabolomics Data, and Evaluation of Anticancer Activity of Cannabis sativa Leaf Extracts

Camargo,

Santamaria-Torres,

Cala

et al. 2023

Metabolites

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Over the past decades, Colombia has suffered complex social problems related to illicit crops, including forced displacement, violence, and environmental damage, among other consequences for vulnerable populations. Considerable effort has been made in the regulation of illicit crops, predominantly Cannabis sativa, leading to advances such as the legalization of medical cannabis and its derivatives, the improvement of crops, and leaving an open window to the development of scientific knowledge to explore alternative uses. It is estimated that C. sativa can produce approximately 750 specialized secondary metabolites. Some of the most relevant due to their anticancer properties, besides cannabinoids, are monoterpenes, sesquiterpenoids, triterpenoids, essential oils, flavonoids, and phenolic compounds. However, despite the increase in scientific research on the subject, it is necessary to study the primary and secondary metabolism of the plant and to identify key pathways that explore its great metabolic potential. For this purpose, a genome-scale metabolic reconstruction of C. sativa is described and contextualized using LC-QTOF-MS metabolic data obtained from the leaf extract from plants grown in the region of Pesca-Boyaca, Colombia under greenhouse conditions at the Clever Leaves facility. A compartmentalized model with 2101 reactions and 1314 metabolites highlights pathways associated with fatty acid biosynthesis, steroids, and amino acids, along with the metabolism of purine, pyrimidine, glucose, starch, and sucrose. Key metabolites were identified through metabolomic data, such as neurine, cannabisativine, cannflavin A, palmitoleic acid, cannabinoids, geranylhydroquinone, and steroids. They were analyzed and integrated into the reconstruction, and their potential applications are discussed. Cytotoxicity assays revealed high anticancer activity against gastric adenocarcinoma (AGS), melanoma cells (A375), and lung carcinoma cells (A549), combined with negligible impact against healthy human skin cells.

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“…Total metabolites were extracted from another stored homogeneous sample. Metabolite extraction references previous study with slight modifications (Du et al ., 2021), detailed information is provided in Methods S2.…”

Section: Methodsmentioning

confidence: 99%

“…However, few reports are associated with plant tissue arrays, which hinders the rapid characterization of analytes in plant tissue samples. Moreover, it is well known that plant growth regulators (PGRs) play many essential roles in plant tissue culture and involve various growth phases (Du et al ., 2021). Indeed, diverse metabolic profiles are significantly affected and undergo dramatic changes in plant tissues with PGR treatment (Khan et al ., 2019; Raj et al ., 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, other derived approaches include desorption electrospray ionization MS (DESI‐MS) (Tata et al ., 2015), direct analysis in real‐time MS (DART‐MS) (Pan et al ., 2013), have also been reported in plant metabolomic studies. Among them, chromatography‐based MS techniques (LC–MS and GC–MS) have attracted increasing attention due to their high‐separation efficiency, high‐detection sensitivity, and high‐throughput data acquisition, and they have been regarded as the most widely used metabolomic analysis strategies in plant science (Du et al ., 2021; Ma and Qi, 2021). LC–MS and GC–MS are commonly used tools for the analysis of metabolites with different properties (e.g.…”

Section: Introductionmentioning

confidence: 99%

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High‐throughput MALDI‐MSI metabolite analysis of plant tissue microarrays

Huang

Liu

et al. 2023

Plant Biotechnology Journal

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SummaryA novel metabolomics analysis technique, termed matrix‐assisted laser desorption/ionization mass spectrometry imaging‐based plant tissue microarray (MALDI‐MSI‐PTMA), was successfully developed for high‐throughput metabolite detection and imaging from plant tissues. This technique completely overcomes the disadvantage that metabolites cannot be accessible on an intact plant tissue due to the limitations of the special structures of plant cells (e.g. epicuticular wax, cuticle and cell wall) through homogenization of plant tissues, preparation of PTMA moulds and matrix spraying of PTMA sections. Our study shows several properties of MALDI‐MSI‐PTMA, including no need of sample separation and enrichment, high‐throughput metabolite detection and imaging (>1000 samples per day), high‐stability mass spectrometry data acquisition and imaging reconstruction and high reproducibility of data. This novel technique was successfully used to quickly evaluate the effects of two plant growth regulator treatments (i.e. 6‐benzylaminopurine and N‐phenyl‐N′‐1,2,3‐thiadiazol‐5‐ylurea) on endogenous metabolite expression in plant tissue culture specimens of Dracocephalum rupestre Hance (D. rupestre). Intra‐day and inter‐day evaluations indicated that the metabolite data detected on PTMA sections had good reproducibility and stability. A total of 312 metabolite ion signals in leaves tissues of D. rupestre were detected, of which 228 metabolite ion signals were identified, they were composed of 122 primary metabolites, 90 secondary metabolites and 16 identified metabolites of unknown classification. The results demonstrated the advantages of MALDI‐MSI‐PTMA technique for enhancing the overall detection ability of metabolites in plant tissues, indicating that MALDI‐MSI‐PTMA has the potential to become a powerful routine practice for high‐throughput metabolite study in plant science.

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UPLC–MS-Based Non-targeted Analysis of Endogenous Metabolite Changes in the Leaves of Scabiosa tschiliensis Grüning Induced by 6-Benzylaminopurine and Kinetin

Cited by 7 publications

References 50 publications

Farm or Lab? A Comparative Study of Oregano’s Leaf and Callus Volatile Isolates Chemistry and Cytotoxicity

Farm or Lab? A Comparative Study of Oregano’s Leaf and Callus Volatile Isolates Chemistry and Cytotoxicity

Genome-Scale Metabolic Reconstruction, Non-Targeted LC-QTOF-MS Based Metabolomics Data, and Evaluation of Anticancer Activity of Cannabis sativa Leaf Extracts

High‐throughput MALDI‐MSI metabolite analysis of plant tissue microarrays

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