UPLC-DAD-MS/MS Method for Analysis of PQQ in Fermentation Broth

Yang, Xuepeng; Li, Pan-Pan; Hu, Xianmei; Ye, Jianbin; Yin, Li; Ma, Ke; Mao, Duobin

doi:10.1007/s10337-015-2932-9

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…These ndings align with previous studies that have reported comparable validation parameters for the determination of PQQ in different matrices. 6,7 The developed method provides a reliable and accurate approach for the quantication of PQQ, crucial in various elds, including pharmaceutical analysis, food safety, and environmental monitoring. The established linear range and high correlation coefficient permit precise PQQ quantication across a wide concentration range.…”

Section: Analysis Methodologymentioning

confidence: 99%

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Selective extraction and quantitative analysis of pyrroloquinoline quinone from food

Ma,

Su,

Pei

et al. 2024

Anal. Methods

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Section: Analysis Methodologymentioning

confidence: 99%

“…6 However, the complexity of food matrices and the low concentration of PQQ make it difficult to achieve satisfactory extraction and enrichment using conventional methods. 7 Therefore, there is a pressing need to develop innovative sorbents capable of selectively extracting and enriching PQQ from food samples.…”

Section: Introductionmentioning

confidence: 99%

Selective extraction and quantitative analysis of pyrroloquinoline quinone from food

Ma,

Su,

Pei

et al. 2024

Anal. Methods

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“…To preparation PQQ and reused extraction solvent, the extraction solvent and stripping agent (1:1, v/v) were put in conical flask, and vibrated for 30 min under 28°C. Analysis method of PQQ According to the literature reported by Yang [13]. The procedure is described as below:…”

Section: Microorganisms and Fermentation Conditionsmentioning

confidence: 99%

An Efficient Separation of Pyrroloquinoline Quinone Using Chemical Complexation Extraction

Yang¹,

Li²,

Wang³

et al. 2015

Proceedings of the International Conference on Chemical, Material and Food Engineering

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Abstract-Pyrroloquinoline quinone (PQQ), an important natural product, is biosynthesized by Gluconobacter oxydans.In order to prepare the product in large scale, a rapid and selective chemical complexation extraction (CCE) is proposed to separate and purify PQQ from fermentation broth. In this work, the effect of extraction solvent system, stripping agent, and inorganic ion on the extraction were investigated, respectively. As a result, trioctylamine-octanol system showed the most extraction constants (log K ex = 5.07). Ammonia water was the most stripping agent (log K ex = 4.85). Several inorganic salts were determined to have negative effect on the extraction, which suggest that inorganic salts should be removed from broth before extraction. Finally, the optimal extraction condition is Trioctylamine-octanol system (1:1, v/v), 28 °C, and pH=6.5, which leads to more than 90% of extraction rate. In conclusion, trioctylamine-octanol system and ammonia water showed excellent extraction efficiency, which can be used to prepare PQQ.

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Bioconversion of recombinantly produced precursor peptide pqqA into pyrroloquinoline quinone (PQQ) using a cell-free in vitro system

Wang

Zhou

et al. 2021

Protein Expression and Purification

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UPLC-DAD-MS/MS Method for Analysis of PQQ in Fermentation Broth

Cited by 3 publications

References 14 publications

Selective extraction and quantitative analysis of pyrroloquinoline quinone from food

Selective extraction and quantitative analysis of pyrroloquinoline quinone from food

An Efficient Separation of Pyrroloquinoline Quinone Using Chemical Complexation Extraction

Bioconversion of recombinantly produced precursor peptide pqqA into pyrroloquinoline quinone (PQQ) using a cell-free in vitro system

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