Abstract:The "uphill (against the concentration gradient)" accumulation of a hydrophobic cation (rhodamine 6G, R6G + ) into the inner phase of a giant unilamellar vesicle (GUV) was realized with the concentration gradient of the counter anion (X − = ClO 4 − , BF 4 − , or Br − ) in the presence of phosphate buffer (P − , pH = 7) in the inner and outer phase of the GUV and detected as the increase of the R6G + fluorescence intensity in the inner phase using a confocal laser scanning fluorescence microscope. The addition … Show more
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