Upgrading of bio‐separation and bioanalysis using synthetic polymers: Molecularly imprinted polymers (MIPs), cryogels, stimuli‐responsive polymers

Abstract

Bio-separation plays a crucial role in many areas. Different polymers are suitable for bio-separation and are useful for applications in applications in both science and technology. Besides biopolymers, there are a broad spectrum of synthetic polymers with tailor-made properties. The synthetic polymers are characterized by their charges, solubility, hydrophilicity/hydrophobicity, sensitivity to environmental conditions and stability. Furthermore, ongoing developments are of great interest on biodegradable polymers for the treatment of diseases.Smart polymers have gained great attention due to their unique characteristics especially emphasizing simultaneously changing their chemical and physical property upon exposure to changes in environmental conditions. In this review, methodologies applied in bio-separation using synthetic polymers are discussed and efficient candidates are focused for the construction of synthetic polymers.

The specific biopanning of single‐domain antibody against haptens based on a functionalized cryogel

“…It was reported that the M13 phage has a length of about 930 nm and a width of about 6 nm, 31 which was much smaller than the pore size of the cryogels, so it could be facilitated the binding of the phages to the cryogels and the passage of the phages through the cryogels. Moreover, cryogels with properties such as elasticity, chemical stability, and biocompatibility could make it widely used for chromatographic elution of biological particles 32 . In addition, AGE was used to insert reactive epoxy groups on the surface of frozen gels for coupling EDA.…”

“…Moreover, cryogels with properties such as elasticity, chemical stability, and biocompatibility could make it widely used for chromatographic elution of biological particles. 32 In addition, AGE was used to insert reactive epoxy groups on the surface of frozen gels for coupling EDA.…”

The Specific Biopanning of Single-domain Antibody Against Haptens Based on a Functionalized Cryogel

“…Moreover, cryogels had properties such as elasticity, chemical stability and biocompatibility and were therefore widely used for chromatographic elution of biological particles. [32] In addition, AGE was used to insert reactive epoxy groups on the surface of frozen gels for coupling EDA.…”

Magnetic molecularly imprinted polymer for trace pharmaceutical analysis: Preconcentration and the electrochemical determination of sufentanil in biological fluids

“…MIPs are considered interesting candidates owing to their prominent privileges, such as reversible binding capacity, great specificity, and applicability in harsh environments. [ 17 ] In addition, magnetic nanoparticles have attracted a great deal of interest due to their great potential application in enzyme immobilization, [ 18 ] bio‐separation, [ 19 ] targeted drug delivery, [ 20 ] and biosensors. [ 21 ] Recently, there has been substantial research on magnetic molecularly imprinted polymers (MMIPs) for selective recognition and removal of targets from the complex matrix.…”