Updates to the recently introduced family Lacipirellulaceae in the phylum Planctomycetes: isolation of strains belonging to the novel genera Aeoliella, Botrimarina, Pirellulimonas and Pseudobythopirellula and the novel species Bythopirellula polymerisocia and Posidoniimonas corsicana

Wiegand, Sandra; Jogler, Mareike; Boedeker, Christian; Heuer, Anja; Peeters, Stijn H.; Kallscheuer, Nicolai; Jetten, Mike S. M.; Kaster, Anne‐Kristin; Rohde, Manfred; Jogler, Christian

doi:10.1007/s10482-020-01486-3

Cited by 47 publications

(13 citation statements)

References 63 publications

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“…Nevertheless, the first two described planctomycetal isolates from macroalgae belong to the class Phycisphaerae [ 24 , 25 , 26 ]. In recent years, single-cell genomes [ 27 ] and axenic cultures from macroalgae were also obtained for the families Pirellulaceae [ 28 , 29 , 30 , 31 ], Lacipirellulaceae [ 32 ], Planctomycetaceae [ 33 , 34 , 35 ], and Isosphaeraceae [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Bacterial Communities on North Sea Macroalgae and Characterization of the Isolated Planctomycetes Adhaeretor mobilis gen. nov., sp. nov., Roseimaritima multifibrata sp. nov., Rosistilla ulvae sp. nov. and Rubripirellula lacrimiformis sp. nov.

Wiegand

Rast²,

Kallscheuer

et al. 2021

Microorganisms

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Planctomycetes are bacteria that were long thought to be unculturable, of low abundance, and therefore neglectable in the environment. This view changed in recent years, after it was shown that members of the phylum Planctomycetes can be abundant in many aquatic environments, e.g., in the epiphytic communities on macroalgae surfaces. Here, we analyzed three different macroalgae from the North Sea and show that Planctomycetes is the most abundant bacterial phylum on the alga Fucus sp., while it represents a minor fraction of the surface-associated bacterial community of Ulva sp. and Laminaria sp. Especially dominant within the phylum Planctomycetes were Blastopirellula sp., followed by Rhodopirellula sp., Rubripirellula sp., as well as other Pirellulaceae and Lacipirellulaceae, but also members of the OM190 lineage. Motivated by the observed abundance, we isolated four novel planctomycetal strains to expand the collection of species available as axenic cultures since access to different strains is a prerequisite to investigate the success of planctomycetes in marine environments. The isolated strains constitute four novel species belonging to one novel and three previously described genera in the order Pirellulales, class Planctomycetia, phylum Planctomycetes.

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Section: Introductionmentioning

confidence: 99%

Analysis of Bacterial Communities on North Sea Macroalgae and Characterization of the Isolated Planctomycetes Adhaeretor mobilis gen. nov., sp. nov., Roseimaritima multifibrata sp. nov., Rosistilla ulvae sp. nov. and Rubripirellula lacrimiformis sp. nov.

Wiegand

Rast²,

Kallscheuer

et al. 2021

Microorganisms

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“…The analysis of the partial 16S rRNA gene sequence placed strain ICT_H6.2 T into the family Lacipirellulaceae , order Pirellulales , class Planctomycetia , phylum Planctomycetota . Its closest type strain is the only described species of the genus Aeoliella , Aeoliella mucimassa Pan181 T [27], with 98.2 % similarity of the 16S rRNA gene, which is below the threshold proposed for species delineation (98.7 %) [37]. The 16S rRNA gene based phylogenetic tree showed that strain ICT_H6.2 T clusters in the family Lacipirellulaceae near strain Pan181 T but forms a separate branch.…”

Section: Partial 16s Rrna Gene Sequence-based Phylogenymentioning

confidence: 99%

“…Growth occurred at 10–37 °C (optimal, 20–30 °C), 0.5–4.0% NaCl (w/v) and pH 6.5–10.0 (optimal, pH 6.5–9.0), overall similar to A. mucimassa Pan181 T [27] (although no data is available regarding its salinity range). Utilized carbon sources (0.1 % w/v) consisted of dextran, arabinose, xylose, cellobiose, mannitol, lactose, galactose, fructose and glucose.…”

Section: Ecology Morphology and Physiologymentioning

confidence: 99%

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Aeoliella straminimaris sp. nov., a novel member of the phylum Planctomycetota with an unusual filamentous structure

Vitorino

Lobo‐da‐Cunha

Vasconcelos

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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Organisms with distinctive biological features and cellular organization constitute the bacterial phylum Planctomycetota . In this study, we formally describe a novel isolate, strain ICT_H6.2T, isolated from sediment samples collected in the brackish environment of the Tagus River estuary (Portugal) using an iChip-based culturing technique. The 16S rRNA gene analysis placed this strain into the phylum Planctomycetota and family Lacipirellulaceae , with a similarity value of 98.0 % to its closest relative Aeoliella mucimassa Pan181T, the currently only known member of the genus. Strain ICT_H6.2T has a genome size of 7.8 Mbp and a DNA G+C content of 59.6 mol %. Strain ICT_H6.2T is heterotrophic, aerobic and capable of microaerobic growth. This strain grows from 10 to 37 °C and from pH 6.5 to 10.0, requires salt to grow, and can tolerate up to 4 % (w/v) NaCl. Diverse nitrogen and carbon sources are utilized for growth. Morphologically, strain ICT_H6.2T is white to beige pigmented, spherical to ovoid in shape and around 1.4×1.1 µm in size. The strain clusters mainly in aggregates and younger cells show motility. Ultrastructural studies showed a cell plan with cytoplasmatic membrane invaginations and unusual filamentous structures with hexagonal organization in transversal section. Morphological, physiological and genomic comparison between strain ICT_H6.2T and its closest relatives strongly suggests it represents a novel species within the genus Aeoliella , for which we propose the name Aeoliella straminimaris sp. nov., represented by strain ICT_H6.2T as the type strain (=CECT 30574T=DSM 114064T).

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“…13 The effective publication states that the type strain was also deposited as ATCC BAA-1503, but no documentation was supplied. 14 Denitrificimonas caeni Saati-Santamaría et al 2021 is a later homotypic synonym of Thiopseudomonas caeni Rudra and Gupta 2021. 15 The effective publication states that the type strain was also deposited as CCTCC AB208156 and KCTC 22292, but no documentation was supplied.…”

mentioning

confidence: 99%

Valid publication of new names and new combinations effectively published outside the IJSEM

Oren

2021

International Journal of Systematic and Evolutionary Microbiology

377

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Updates to the recently introduced family Lacipirellulaceae in the phylum Planctomycetes: isolation of strains belonging to the novel genera Aeoliella, Botrimarina, Pirellulimonas and Pseudobythopirellula and the novel species Bythopirellula polymerisocia and Posidoniimonas corsicana

Cited by 47 publications

References 63 publications

Analysis of Bacterial Communities on North Sea Macroalgae and Characterization of the Isolated Planctomycetes Adhaeretor mobilis gen. nov., sp. nov., Roseimaritima multifibrata sp. nov., Rosistilla ulvae sp. nov. and Rubripirellula lacrimiformis sp. nov.

Analysis of Bacterial Communities on North Sea Macroalgae and Characterization of the Isolated Planctomycetes Adhaeretor mobilis gen. nov., sp. nov., Roseimaritima multifibrata sp. nov., Rosistilla ulvae sp. nov. and Rubripirellula lacrimiformis sp. nov.

Aeoliella straminimaris sp. nov., a novel member of the phylum Planctomycetota with an unusual filamentous structure

Valid publication of new names and new combinations effectively published outside the IJSEM

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