Updates on the surface antigens of basophils: <scp>CD</scp>16 on basophils of patients with respiratory or insect venom allergy and the rejection of <scp>CD</scp>203c and <scp>CD</scp>63 externalization decoupling by bisindolylmaleimides

Heneberg, Petr; Riegerová, Kamila; Říhová, Adéla; Šimčíková, Daniela; Kučera, Petr

doi:10.1111/cea.13288

Cited by 5 publications

(4 citation statements)

References 31 publications

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“…Another important tool for the diagnosis of immediate hypersensitivity reactions is the basophil activation test (BAT). The BAT is a common method frequently used in diagnosing a venom response, and it is considered to be a standard approach in experimental studies [8,9]. Because of its high specificity and sensitivity, the BAT has also been successfully applied in VIT monitoring.…”

Section: Introductionmentioning

confidence: 99%

Association between Venom Immunotherapy and Changes in Serum Protein—Peptide Patterns

et al. 2021

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Venom immunotherapy (VIT) is administered to allergic patients to reduce the risk of dangerous systemic reactions following an insect sting. To better understand the mechanism of this treatment and its impact on the human organism, we analysed serum proteomic patterns obtained at five time-points from Hymenoptera-venom-allergic patients undergoing VIT. For statistical analyses, patients were additionally divided into two groups (high responders and low responders) according to serum sIgG4 levels. VIT was found to be associated with changes in seven proteins: the fibrinogen alpha chain, complement C4-A, complement C3, filamin-B, kininogen-1, myosin-9 and inter-alpha-trypsin inhibitor heavy chain H1. The number of discriminative m/z (mass-to-charge ratio) features increased up to the 90th day of VIT, which may be associated with the development of immunity after the administration of increased venom doses. It may also suggest that during VIT, there may occur processes involved not only in protein synthesis but also in protein degradation (caused by proteolytic venom components). The results are consistent with measured serum sIgG4 levels, which increased from 2.04 mgA/I at baseline to 7.25 mgA/I at 90 days. Moreover, the major proteomic changes were detected separately in the high responder group. This may suggest that changes in protein–peptide profiles reflect the actual response to VIT.

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Section: Introductionmentioning

confidence: 99%

Association between Venom Immunotherapy and Changes in Serum Protein—Peptide Patterns

et al. 2021

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“…Venom extracts for VIT are drugs and, therefore, need consistent high-quality control and batch-to-batch constant characteristics to ensure safety and efficacy of VIT. In addition, allergen extracts can also be used in diagnostics, for example, for basophil activation tests [29,30]. Here, too, standardized high quality is indispensable.…”

Section: Introductionmentioning

confidence: 99%

Allergen Content of Therapeutic Preparations for Allergen-Specific Immunotherapy of European Paper Wasp Venom Allergy

Grosch

Lesur

Kler

et al. 2022

Toxins

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Allergy to Polistes dominula (European paper wasp) venom is of particular relevance in Southern Europe, potentially becoming a threat in other regions in the near future, and can be effectively cured by venom immunotherapy (VIT). As allergen content in extracts may vary and have an impact on diagnostic and therapeutic approaches, the aim was to compare five therapeutic preparations for VIT of P. dominula venom allergy available in Spain. Products from five different suppliers were analyzed by SDS-PAGE and LC-MS/MS and compared with a reference venom sample. Three products with P. dominula venom and one product with a venom mixture of American Polistes species showed a comparable band pattern in SDS-PAGE as the reference sample and the bands of the major allergens phospholipase A1 and antigen 5 were assignable. The other product, which consists of a mixture of American Polistes species, exhibited the typical band pattern in one, but not in another sample from a second batch. All annotated P. dominula allergens were detected at comparable levels in LC-MS/MS analysis of products containing P. dominula venom. Due to a lack of genomic information on the American Polistes species, the remaining products were not analyzed by this method. The major Polistes allergens were present in comparable amounts in the majority, but not in all investigated samples of venom preparations for VIT of P. dominula venom allergy.

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“…Over the years, CD13, CD44, CD54, CD63, CD69, CD107a, CD123, CD164, CCR3, CD203c, TLR-4, and FcεRI have been recognized as identification markers of basophils (He et al, 2022). The newly emerging markers of subpopulations of basophils, like CD16b, have also been reported (Heneberg et al, 2019). Some of these markers such as CD203c and CD63 (Abuaf et al, 2008;Imamura et al, 2021) are upregulated upon activation.…”

Section: Introductionmentioning

confidence: 99%

Increased expressions of CD123, CD63, CD203c, and Fc epsilon receptor I on blood leukocytes of allergic asthma

Xie

Chen

Zhang

et al. 2022

Front. Mol. Biosci.

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Background: Altered basophil identification markers have been discovered to associate with allergic asthma (AA) in recent years. However, little is known about the expression of basophil markers in blood granulocytes.Aim: To parallel test blood basophils in peripheral blood mononuclear cell (PBMC) and granulocyte populations of patients with AA and AA combined with allergic rhinitis (ARA)Methods: The expressions of surface molecules were determined via flow cytometry. CD123 expressing cells in blood were isolated using a cell sorting technique, and mouse AA models were employed for in vivo study.Results: The numbers of CD123+HLA-DR− cells in the granulocytes of AA and ARA patients markedly increased. However, only 49.7% of CD123+HLA-DR− cells in granulocytes and 99.0% of CD123+HLA-DR− cells in PBMCs were basophils. Almost all CD123+HLA-DR− cells expressed CD63 regardless in granulocytes or PBMC. The numbers of CD63, Fc epsilon receptor I (FcεRI), and CD203c expressing cells markedly enhanced in CD123+HLA-DR− granulocytes of AA and ARA patients. Mean fluorescence intensity (MFI) of CD63 and CD203c expressions on CD123+HLA-DR− PBMC and granulocytes of AA and ARA patients dramatically elevated. House dust mite extract (HDME) and Artemisia sieversiana wild allergen extract (ASWE) enhanced the numbers of CD63+CD123+HLA-DR− granulocytes and PBMC and the MFI of CD203c expression on CD123+HLA-DR− granulocyte of AA and ARA patients. Histamine, tryptase, and PGD2 enhanced proportions of CD123+ KU812 cells. ASWE- and HDME-induced AA mice showed upregulated CD63 expression on basophils. In conclusion, upregulated expressions of CD123, CD203c, CD63, and FcεRIα in PBMC and granulocytes of patients with AA and ARA suggest that CD123+HLA-DR− cells may contribute to the development of AA and ARA.

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Updates on the surface antigens of basophils: CD16 on basophils of patients with respiratory or insect venom allergy and the rejection of CD203c and CD63 externalization decoupling by bisindolylmaleimides

Cited by 5 publications

References 31 publications

Association between Venom Immunotherapy and Changes in Serum Protein—Peptide Patterns

Association between Venom Immunotherapy and Changes in Serum Protein—Peptide Patterns

Allergen Content of Therapeutic Preparations for Allergen-Specific Immunotherapy of European Paper Wasp Venom Allergy

Increased expressions of CD123, CD63, CD203c, and Fc epsilon receptor I on blood leukocytes of allergic asthma

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