Updated inventory, evolutionary and expression analyses of G (PDR) type ABC transporter genes of rice

Gupta, Bidya Bhushan; Selter, Liselotte L.; Baranwal, Vinay Kumar; Arora, Deepanksha; Mishra, Sumit Kumar; Sirohi, Parul; Poonia, Anuj Kumar; Chaudhary, Richa; Kumar, Rahul; Krattinger, Simon G.; Chauhan, Harsh

doi:10.1016/j.plaphy.2019.08.004

Cited by 18 publications

(7 citation statements)

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“…To elucidate the regulatory function(s) of OsMYB60 in cuticular wax deposition, we examined the expression of wax biosynthesis-associated genes (OsCER1, OsCER4, OsGL1-8, OsFATB1, WSL4, DWA1, OsWR1, and OsLACS1) and wax transporter genes (OsPDR8, OsPDR9, and OsABCG9) (Gupta et al, 2019;Islam et al, 2009;Mao et al, 2012;Ni et al, 2018;Wang et al, 2012;Wang et al, 2017;Zhou et al, 2014;Zhu & Xiong, 2013) in the leaves of WT and osmyb60 plants after 3 days of drought stress their expression levels were significantly lower in the os-myb60 mutants compared to the WT under drought conditions (Figure 6d-f). However, there was no significant difference in the expression of WSL4, DWA1, OsWR1, OsLACS1, and OsABCG9 between WT and osmyb60 mutants under both normal and drought conditions (Figure S6).…”

Section: Loss Of Function Of Osmyb60 Downregulates Expression Of Wax ...mentioning

confidence: 99%

Mutation of OsMYB60 reduces rice resilience to drought stress by attenuating cuticular wax biosynthesis

et al. 2022

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SUMMARY The cuticular wax layer on leaf surfaces limits non‐stomatal water loss to the atmosphere and protects against pathogen invasion. Although many genes associated with wax biosynthesis and wax transport in plants have been identified, their regulatory mechanisms remain largely unknown. Here, we show that the MYB transcription factor OsMYB60 positively regulates cuticular wax biosynthesis and this helps rice (Oryza sativa) plants tolerate drought stress. Compared with the wild type (japonica cultivar ‘Dongjin’), osmyb60 null mutants (osmyb60‐1 and osmyb60‐2) exhibited increased drought sensitivity, with more chlorophyll leaching and higher rates of water loss. Quantitative reverse‐transcription PCR showed that the loss of function of OsMYB60 led to downregulation of wax biosynthesis genes, leading to reduced amounts of total wax components on leaf surfaces under normal conditions. Yeast one‐hybrid, luciferase transient transcriptional activity, and chromatin immunoprecipitation assays revealed that OsMYB60 directly binds to the promoter of OsCER1 (a key gene involved in very‐long‐chain alkane biosynthesis) and upregulates its expression. Taken together, these results demonstrate that OsMYB60 enhances rice resilience to drought stress by promoting cuticular wax biosynthesis on leaf surfaces.

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Section: Loss Of Function Of Osmyb60 Downregulates Expression Of Wax ...mentioning

confidence: 99%

Mutation of OsMYB60 reduces rice resilience to drought stress by attenuating cuticular wax biosynthesis

et al. 2022

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“…ABCG proteins are the G type of ATP-binding cassette (ABC) transporters, which can transport a variety of metabolites, including monolignols, hormones, and sterol glycosides. ABCG genes have been identified in many plants such as Arabidopsis thaliana [ 22 ], rice ( Oryza sativa ) [ 23 ], corn ( Zea mays ) [ 24 ], alfalfa ( Medicago truncatula ) [ 25 ], grapes ( Vitis vinifera ) [ 26 ], pineapple ( Ananas comosus ) [ 27 ], and tomato ( Solanum lycopersicum ) [ 28 ]. AtABCG14 plays an important role in delivering cytokinin from the root to the shoot.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the ABC Transporter G Subfamily in Pomegranate and Function Analysis of PgrABCG14

Qin

et al. 2022

IJMS

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ATP-binding cassette subfamily G (ABCG) proteins play important roles in plant growth and development by transporting metabolites across cell membranes. To date, the genetic characteristics and potential functions of pomegranate ABCG proteins (PgrABCGs) have remained largely unknown. In this study, we found that 47 PgrABCGs were divided into five groups according to a phylogenetic analysis; groups I, II, III, and IV members are half-size proteins, and group V members are full-size proteins. PgrABCG14, PgrABCG21, and PgrABCG47 were highly expressed in the inner seed coat but had very low expression levels in the outer seed coat, and the expression levels of these three PgrABCG genes in the inner seed coats of hard-seeded pomegranate ‘Dabenzi’ were higher than those of soft-seeded pomegranate ‘Tunisia’. In addition, the expression of these three PgrABCG genes was highly correlated with the expression of genes involved in lignin biosynthesis and hormone signaling pathways. The evolution of PgrABCG14 presents a highly similar trend to the origin and evolution of lignin biosynthesis during land plant evolution. Ectopic expression of PgrABCG14 in Arabidopsis promoted plant growth and lignin accumulation compared to wild type plants; meanwhile, the expression levels of lignin biosynthesis-related genes (CAD5, C4H, and Prx71) and cytokinin response marker genes (ARR5 and ARR15) were significantly upregulated in transgenic plants, which suggests the potential role of PgrABCG14 in promoting plant growth and lignin accumulation. Taken together, these findings not only provide insight into the characteristics and evolution of PgrABCGs, but also shed a light on the potential functions of PgrABCGs in seed hardness development.

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“…3 a) comparing with G. max , L. angustifolius , and A. thaliana (Additional file 5 ). For instance, we found a total of 66 ABCG members in L. albus (Additional file 4 ), while 43 in A. thaliana (Additional file 6 ) were previously reported [ 9 , 41 ], indicating expansion of ABCG subfamily in legumes. Additionally, we compared gene duplication events of ABCG subfamily genes within the L. albus genome and with A. thaliana, G. max, and L. angustifolius genomes (Additional file 5 ).…”

Section: Discussionmentioning

confidence: 50%

Identification of ABC transporter G subfamily in white lupin and functional characterization of L.albABGC29 in phosphorus use

et al. 2021

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Background White lupin (Lupinus albus) is a leguminous crop with elite adaptive ability in phosphorus-deficient soil and used as a model plant for studying phosphorus (P) use. However, the genetic basis of its adaptation to low P (LP) remains unclear. ATPase binding cassette (ABC) transports G subfamily play a crucial role in the transportation of biological molecules across the membrane. To date, identification of this subfamily has been analyzed in some plants, but no systematic analysis of these transporters in phosphorus acquisition is available for white lupin. Results This study identified 66 ABCG gene family members in the white lupin genome using comprehensive approaches. Phylogenetic analysis of white lupin ABCG transporters revealed six subclades based on their counterparts in Arabidopsis, displaying distinct gene structure and motif distribution in each cluster. Influences of the whole genome duplication on the evolution of L.albABCGs were investigated in detail. Segmental duplications appear to be the major driving force for the expansion of ABCGs in white lupin. Analysis of the Ka/Ks ratios indicated that the paralogs of the L.albABCG subfamily members principally underwent purifying selection. However, it was found that L.albABCG29 was a result of both tandem and segmental duplications. Overexpression of L.albABCG29 in white lupin hairy root enhanced P accumulation in cluster root under LP and improved plant growth. Histochemical GUS staining indicated that L.albABCG29 expression increased under LP in white lupin roots. Further, overexpression of L.albABCG29 in rice significantly improved P use under combined soil drying and LP by improving root growth associated with increased rhizosheath formation. Conclusion Through systematic and comprehensive genome-wide bioinformatics analysis, including conserved domain, gene structures, chromosomal distribution, phylogenetic relationships, and gene duplication analysis, the L.albABCG subfamily was identified in white lupin, and L.albABCG29 characterized in detail. In summary, our results provide deep insight into the characterization of the L.albABCG subfamily and the role of L.albABCG29 in improving P use.

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Updated inventory, evolutionary and expression analyses of G (PDR) type ABC transporter genes of rice

Abstract: , H. (2019). Updated inventory, evolutionary and expression analyses of G (PDR) type ABC transporter genes of rice.

Cited by 18 publications

References 50 publications

Mutation of OsMYB60 reduces rice resilience to drought stress by attenuating cuticular wax biosynthesis

Mutation of OsMYB60 reduces rice resilience to drought stress by attenuating cuticular wax biosynthesis

Characterization of the ABC Transporter G Subfamily in Pomegranate and Function Analysis of PgrABCG14

Identification of ABC transporter G subfamily in white lupin and functional characterization of L.albABGC29 in phosphorus use

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