Update on Tissue-Engineered Biological Dressings

Ehrenreich, M.; Ruszczak, Z.

doi:10.1089/ten.2006.12.ft-193

Cited by 18 publications

(28 citation statements)

References 106 publications

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“…However, it is possible that alternative outcome measures or additional time points may elucidate signs of improved or accelerated healing. For example, StrataGraft does not disintegrate in the wound bed like some first generation skin substitutes,9 therefore it is tempting to speculate that it may better condition the wound bed and promote wound healing by more consistent, sustained delivery of bioactive molecules to the wound.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, many of the first-generation living skin substitutes have poor handling characteristics and typically lack epidermal barrier function 7,8. The rapid deterioration observed for some of them in the wound bed9 may compromise the delivery of growth factors and other desirable bioactive molecules.…”

mentioning

confidence: 99%

“…The characteristics of ideal wound dressings or biological skin substitutes were first suggested by Pruitt and Levine14 and have been expanded upon by others in recent years (Table 1)7,9. StrataGraft is a second-generation living skin substitute which possesses many of these characteristics.…”

mentioning

confidence: 99%

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Phase I/II Clinical Evaluation of StrataGraft: A Consistent, Pathogen-Free Human Skin Substitute

Schurr

Foster

Centanni

et al. 2009

Journal of Trauma: Injury, Infection &Amp; Critical Care

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Background-Large wounds often require temporary allograft placement to optimize the wound bed and prevent infection until permanent closure is feasible. We developed and clinically tested a second-generation living human skin substitute (StrataGraft). StrataGraft provides both a dermis and a fully-stratified, biologically-functional epidermis generated from a pathogen-free, long-lived human keratinocyte progenitor cell line, Neonatal Immortalized KeratinocyteS (NIKS).

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Phase I/II Clinical Evaluation of StrataGraft: A Consistent, Pathogen-Free Human Skin Substitute

Schurr

Foster

Centanni

et al. 2009

Journal of Trauma: Injury, Infection &Amp; Critical Care

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“…Recombinant PDGF application to diabetic wounds demonstrates some reproducible improvement of the wound-healing process (7). Apligraf or Dermagraft are both biosynthetic grafts used to promote wound closure in diabetic patients (9,10). While there is evidence to support these therapies’ benefit when used clinically, widespread use is tempered by their prohibitive costs (12).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of stromal cell-derived factor-1α further impairs diabetic wound healing

Bermudez

Herdrich

et al. 2011

Journal of Vascular Surgery

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Objective Impaired diabetic wound healing is associated with abnormal SDF-1α production, decreased angiogenesis, and chronic inflammation. Lentiviral-mediated overexpression of SDF-1α can correct the impairments in angiogenesis and healing in diabetic wounds. We hypothesized that SDF-1α is a critical component of the normal wound healing response and that inhibition of SDF-1α would further delay the wound-healing process. Design of study Db/Db diabetic mice and Db/+ non-diabetic mice were wounded with an 8mm punch biopsy and the wounds treated with a lentiviral vector containing either the GFP or SDF-1α inhibitor transgene. The inhibitor transgene is a mutant form of SDF-1α that binds, but does not activate, the CXCR4 receptor. Computerized planimetry was used to measure wound size daily. Wounds were analyzed at 3 and 7 days by histology and for production of inflammatory markers using real-time PCR. The effect of the SDF-1α inhibitor on cellular migration was also assessed. Results Inhibition of SDF-1α resulted in a significant decrease in the rate of diabetic wound healing, (3.8 cm2/day versus 6.5 cm2/day in GFP-treated wounds p=0.04), and also impaired the early phase of non-diabetic wound healing. SDF-1α inhibition also resulted in fewer small-caliber vessels, less granulation tissue formation, and increased proinflammatory gene expression (IL-6 and MIP-2) in the diabetic wounds. Conclusions The relative level of SDF-1α in the wound plays a key role in the wound healing response. Alterations in the wound level of SDF-1α, as seen in diabetes or by SDF-1α inhibition, impair healing by decreasing cellular migration and angiogenesis, leading to increased production of inflammatory cytokines and inflammation.

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“…Although these composite scaffolds have demonstrated efficacious results in clinical applications, problems with mechanical stability, prolonged healing times, scarring, and limited tissue functionality persist 10. The ultimate success of tissue engineered skin substitutes which promote rapid reepithelialization requires further development of dermal analogs that more closely mimic the wound environment and biochemically direct cellular function 1–3,11…”

Section: Introductionmentioning

confidence: 99%

Designing tailored biomaterial surfaces to direct keratinocyte morphology, attachment, and differentiation

Bush

Driscoll

Soto

et al. 2008

J Biomedical Materials Res

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Precisely engineering the surface chemistry of biomaterials to modulate the adsorption and functionality of biochemical signaling molecules that direct cellular functions is critical in the development of tissue engineered scaffolds. Specifically, this study describes the use of functionalized self-assembled monolayers (SAMs) as a model system to assess the effects of biomaterial surface properties on controlling fibronectin (FN) conformation and concentration as well as keratinocyte function. By systematically analyzing FN adsorption at low and saturated surface densities, we distinguished between SAM dependent effects of FN concentration and conformation on presenting cellular binding domains that direct cellular functions. Quantitative image analyses of immunostained samples showed that modulating the availability of the FN synergy site directly correlated with changes in keratinocyte attachment, spreading, and differentiation, through integrin mediated signaling mechanisms. The results of this study will be used to elucidate design features that can be incorporated into dermal equivalents and percutaneous implants to enhance the rate of reepithelialization and tissue regeneration. Furthermore, these findings indicate that SAM based model systems are a valuable tool for designing and investigating the development of scaffolds that regulate the conformation of extracellular matrix cues and cellular functions that accelerate the rate of tissue regeneration.

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Update on Tissue-Engineered Biological Dressings

Cited by 18 publications

References 106 publications

Phase I/II Clinical Evaluation of StrataGraft: A Consistent, Pathogen-Free Human Skin Substitute

Phase I/II Clinical Evaluation of StrataGraft: A Consistent, Pathogen-Free Human Skin Substitute

Inhibition of stromal cell-derived factor-1α further impairs diabetic wound healing

Designing tailored biomaterial surfaces to direct keratinocyte morphology, attachment, and differentiation

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