Update on solid-phase extraction for the analysis of lipid classes and related compounds

Ruı́z-Gutı́errez, Valentina; Pérez‐Camino, María del Carmen

doi:10.1016/s0021-9673(00)00181-3

Cited by 127 publications

(58 citation statements)

References 182 publications

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“…5) (22), affecting the quantitative recovery of both n-GSLs and n-lysoGSLs, and resulting in the loss of other lipid classes. Using an aminopropyl solid phase column, all cellular lipids are recovered, and can be stored for further analysis; aminopropyl columns are preferable to silicic acid columns as they are slightly less polar (56), resulting in recovery of n-lyso-GSLs and n-GSLs in the same fraction. Use of different aminopropyl solid phase column sizes, from between 100-500 mg, renders the method useful for separation of different amounts of cellular lipids, ranging between 4-20 mg (46).…”

Section: Discussionmentioning

confidence: 99%

Aminopropyl solid phase extraction and 2 D TLC of neutral glycosphingolipids and neutral lysoglycosphingolipids

Bodennec

Pelled

Futerman

2003

Journal of Lipid Research

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Methods for isolation of neutral lysoglycosphingolipids (n-lyso-GSLs) such as glucosylsphingosine and galactosylsphingosine normally involve mild alkaline or acid hydrolysis followed by multiple chromatography steps, yielding relatively low recoveries of n-lyso-GSLs and neutral glycosphingolipids (n-GSLs). We now describe a new technique for isolating these compounds using one chromatography step, resulting in quantitative recovery of n-GSLs and n-lysoGSLs. Lipids are extracted using a modified Folch procedure in which recovery is optimized by reextracting the Folch upper phase with water-saturated butanol. The extract is applied to an aminopropyl solid phase column from which both n-GSLs and n-lyso-GSLs elute in the same fraction. Separation is achieved using a new two-dimensional thin-layer chromatography procedure. The usefulness of this technique for biological samples was tested by examining Glc

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Section: Discussionmentioning

confidence: 99%

Aminopropyl solid phase extraction and 2 D TLC of neutral glycosphingolipids and neutral lysoglycosphingolipids

Bodennec

Pelled

Futerman

2003

Journal of Lipid Research

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“…To optimize for ceramides, the washed, harvested mycelia were extracted with hexane-ethanol (95:5) by vortexing twice with glass beads (21). The combined supernatants were dried, and solids were dissolved in chloroform and applied to a preconditioned Sep-Pak (Waters) solid-phase extraction (SPE) silica column (63). After a chloroform wash, ceramides were eluted with chloroformmethanol (98:2), followed by chloroform-methanol (95:5), and glycolipids were eluted with chloroform-acetone (1:1).…”

Section: Cell Survival Measurementsmentioning

confidence: 99%

Stress-Induced Cell Death Is Mediated by Ceramide Synthesis in Neurospora crassa

et al. 2008

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The combined stresses of moderate heat shock (45°C) and analog-induced glucose deprivation constitute a lethal stress for Neurospora crassa. We found that this cell death requires fatty acid synthesis and the cofactor biotin. In the absence of the cofactor, the stressed cells are particularly sensitive to exogenous ceramide, which is lethal at low concentrations. When we extracted endogenous sphingolipids, we found that unique ceramides were induced (i) by the inhibitory glucose analog 2-deoxyglucose and (ii) by combined heat shock and 2-deoxyglucose. We determined that the former is a 2-deoxyglucose-modified ceramide. By structural analysis, we identified the latter, induced by dual stress, as C 18 (OH)-phytoceramide. We also identified C 24 (OH)-phytoceramide as a constitutive ceramide that continues to be produced during the combined stresses. The unusual C 18 (OH)-phytoceramide is not made by germinating asexual spores subjected to the same heat and carbon stress. Since these spores, unlike growing cells, do not die from the stresses, this suggests a possible connection between synthesis of the dual-stress-induced ceramide and cell death. This connection is supported by the finding that a (dihydro)ceramide synthase inhibitor, australifungin, renders cells resistant to death from these stresses. The OS-2 mitogen-activated protein kinase, homologous to mammalian p38, may be involved in the cell death signaling pathway. Strains lacking OS-2 survived the combined stresses better than the wild type, and phosphorylated OS-2 increased in wild-type cells in response to heat shock and combined heat and carbon stress.

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“…On the other hand, the demand for fast and reliable quantitative screening methods of complex food matrix extracts has led to the development of more widely applicable detection strategies. With regard to this, the analysis of phospholipids has been performed by several different methods, including thin-layer chromatography (TLC) [21,22,23], highperformance liquid chromatography (HPLC), [24,25,26,27] and solid-phase extraction (SPE) [28,29,30]. In earlier articles, the lipid quantification had been performed exclusively by TLC, but the method has several disadvantages; namely, the separation of individual classes is very difficult and time consuming, and the technique is not always accurate.…”

Section: Methodology Determination Of Phospholipids In Food Samplesmentioning

confidence: 99%

Dietary Phospholipids and Phytostrerols: A Review on Some Nigerian Vegetable Oils

Aremu¹,

Ibrahim²

2017

ijSciences

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Dietary phospholipids and phytosterols have proven to be potential sources of bioactive lipids with widespread effects on pathways related to inflammation, cholesterol metabolism, and high-density lipoprotein function. Due to their biological and physicochemical properties, they are important in human nutrition. The efficient separation and accurate quantification of phospholipids and phytosterols can be achieved with highperformance liquid chromatography-evaporative light scattering detection (HPLC-ELSD) and gas chromatography (GC) often combined with mass spectrometry respectively. The phospholipid and phytosterol compositions of some Nigerian vegetable oils were reviewed. From the literature, the phospholipid concentrations (mg/100) of phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl serine, lysophosphatidyl choline, phosphatidyl inositol and phosphatidic acid are in the range of 2.60 -1168.00, 1.13 -558.00, 0.10 -336.00, 0.

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Update on solid-phase extraction for the analysis of lipid classes and related compounds

Cited by 127 publications

References 182 publications

Aminopropyl solid phase extraction and 2 D TLC of neutral glycosphingolipids and neutral lysoglycosphingolipids

Aminopropyl solid phase extraction and 2 D TLC of neutral glycosphingolipids and neutral lysoglycosphingolipids

Stress-Induced Cell Death Is Mediated by Ceramide Synthesis in Neurospora crassa

Dietary Phospholipids and Phytostrerols: A Review on Some Nigerian Vegetable Oils

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