Update on Neutrophil Function in Severe Inflammation

Mortaz, Esmaeil; Alipoor, Shamila D.; Adcock, Ian M.; Mumby, Sharon; Koenderman, Leo

doi:10.3389/fimmu.2018.02171

Cited by 318 publications

(272 citation statements)

References 163 publications

(236 reference statements)

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“…Other populations of neutrophils identified in inflammatory/infectious conditions include CD63 + [76] or PD-L1 + [77] neutrophils identified in the airways of cystic fibrosis patients; CD64 + neutrophils, that are increased in sepsis or systemic infections (e.g., pneumonia) [78]; neutrophils expressing receptor activator of nuclear factor κΒ ligand (RANKL)a member of the tumor necrosis factor (TNF) superfamilyidentified in the blood of chronic obstructive pulmonary disease patients [79]; CD49d + cysteinyl leukotriene receptor 1 (CysLTR1) + neutrophils present in the nasal lavage fluid of patients with respiratory tract infections caused by different viruses (e.g., influenza virus, respiratory syncytial virus, or rhinovirus) [80]; human leukocyte antigen -DR isotype (HLA-DR) + neutrophils present in Brazilian patients with leishmaniasis [81]; and para-and proinflammatory neutrophil populations that are present in the oral cavity of healthy or chronic periodontal disease subjects, respectively [82,83]. The question remains whether these populations are distinct subsets derived through differentiation or via activation by cytokines present during ongoing disease/inflammatory processes.…”

Section: Trends In Immunologymentioning

confidence: 99%

Neutrophil Diversity in Health and Disease

Silvestre-Roig

Fridlender

Glogauer

et al. 2019

Trends in Immunology

351

335

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Section: Trends In Immunologymentioning

confidence: 99%

Neutrophil Diversity in Health and Disease

Silvestre-Roig

Fridlender

Glogauer

et al. 2019

Trends in Immunology

351

335

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“…These cells exist throughout the body and are recruited from the blood stream to sites of inflammation, including the gut (Borregaard 2010;Fournier and Parkos 2012;Wera et al 2016). While intestinal neutrophil recruitment often occurs due to the presence of pathogens, resulting from acute inflammation, such recruitment can also occur chronically due to aberrant interactions between the immune system and the gut microbiota (Foell et al 2003;Wera et al 2016;Mortaz et al 2018;Rosales 2018;Murdoch and Rawls 2019).…”

mentioning

confidence: 99%

QTL Mapping of Intestinal Neutrophil Variation in Threespine Stickleback Reveals Possible Gene Targets Connecting Intestinal Inflammation and Systemic Health

Beck

Currey

Small

et al. 2020

G3 Genes|Genomes|Genetics

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Selection, via host immunity, is often required to foster beneficial microbial symbionts and suppress deleterious pathogens. In animals, the host immune system is at the center of this relationship. Failed host immune system-microbial interactions can result in a persistent inflammatory response in which the immune system indiscriminately attacks resident microbes, and at times the host cells themselves, leading to diseases such as Ulcerative Colitis, Crohn’s Disease, and Psoriasis. Host genetic variation has been linked to both microbiome diversity and to severity of such inflammatory disease states in humans. However, the microbiome and inflammatory states manifest as quantitative traits, which encompass many genes interacting with one another and the environment. The mechanistic relationships among all of these interacting components are still not clear. Developing natural genetic models of host-microbe interactions is therefore fundamental to understanding the complex genetics of these and other diseases. Threespine stickleback (Gasterosteus aculeatus) fish are a tractable model for attacking this problem because of abundant population-level genetic and phenotypic variation in the gut inflammatory response. Previous work in our laboratory identified genetically divergent stickleback populations exhibiting differences in intestinal neutrophil activity. We took advantage of this diversity to genetically map variation in an emblematic element of gut inflammation - intestinal neutrophil recruitment - using an F2-intercross mapping framework. We identified two regions of the genome associated with increased intestinal inflammation containing several promising candidate genes. Within these regions we found candidates in the Coagulation/Complement System, NFkB and MAPK pathways along with several genes associated with intestinal diseases and neurological diseases commonly accompanying intestinal inflammation as a secondary symptom. These findings highlight the utility of using naturally genetically diverse ‘evolutionary mutant models’ such as threespine stickleback to better understand interactions among host genetic diversity and microbiome variation in health and disease states.

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“…Granulocytes are known for their high potential and efficiency in detecting and eradicating microbial infections through an immune response against invading pathogens [66]. Aiming at granulocytelabeling with nanoparticles for later use in inflammation/infection process detection, different methods of granulocyte isolation were tested, and their labeling with multimodal nanoparticles allowed their internalization and their qualitative and quantitative evaluations.…”

Section: Discussionmentioning

confidence: 99%

Methods of Granulocyte Isolation from Human Blood and Labeling with Multimodal Superparamagnetic Iron Oxide Nanoparticles

et al. 2020

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This in vitro study aimed to find the best method of granulocyte isolation for subsequent labeling with multimodal nanoparticles (magnetic and fluorescent properties) to enable detection by optical and magnetic resonance imaging (MRI) techniques. The granulocytes were obtained from venous blood samples from 12 healthy volunteers. To achieve high purity and yield, four different methods of granulocyte isolation were evaluated. The isolated granulocytes were labeled with multimodal superparamagnetic iron oxide nanoparticles (M-SPIONs) coated with dextran, and the iron load was evaluated qualitatively and quantitatively by MRI, near-infrared fluorescence (NIRF) and inductively coupled plasma mass spectrometry (ICP-MS). The best method of granulocyte isolation was Percoll with Ficoll, which showed 95.92% purity and 94% viability. After labeling with M-SPIONs, the granulocytes showed 98.0% purity with a yield of 3.5 × 10 6 cells/mL and more than 98.6% viability. The iron-loading value in the labeled granulocytes, as obtained by MRI, was 6.40 ± 0.18 pg/cell. Similar values were found with the ICP-MS and NIRF imaging techniques. Therefore, our study shows that it is possible to isolate granulocytes with high purity and yield and labeling with M-SPIONs provides a high internalized iron load and low toxicity to cells. Therefore, these M-SPION-labeled granulocytes could be a promising candidate for future use in inflammation/infection detection by optical and MRI techniques.Hard-to-reach or occult inflammation and infection processes are clinically challenging. Their clinical diagnoses involve biochemical and radiological examinations, but these methods can produce false negatives [11][12][13][14][15]. To determine the localization, extent and severity of inflammation, imaging techniques such as computed tomography (CT) and magnetic resonance imaging (MRI) can be performed but have limitations in the early stages of the disease, as they are dependent on morphological changes [16][17][18]. Accurate and early diagnosis of inflammation and infection helps reduce mortality and morbidity and increase drug treatment success [19][20][21].Proinflammatory effects help leukocytes migrate from the blood vessel to the site of injury, due to increased vascular permeability through stimulation by cytokines and interleukins. There is an initial accumulation of granulocytes, mainly neutrophils, in the inflamed area and later of lymphocytes and macrophages [22][23][24]. Granulocytes are the most abundant subset of leukocytes [25] and are produced in the bone marrow, originating from myeloid precursor cells and undergoing a maturing process before they are able to perform their functions, mainly phagocytosis [25,26]. They differ from other cells in that they have granules with proteolytic enzymes that fight microorganisms or other inflammatory agents [25,27]. Granulocytes are considered frontline cells in inflammation and infection processes [28]. In many cases, to detect inflammation/infection, it is necessary to label isolated granulocyte...

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Update on Neutrophil Function in Severe Inflammation

Cited by 318 publications

References 163 publications

Neutrophil Diversity in Health and Disease

Neutrophil Diversity in Health and Disease

QTL Mapping of Intestinal Neutrophil Variation in Threespine Stickleback Reveals Possible Gene Targets Connecting Intestinal Inflammation and Systemic Health

Methods of Granulocyte Isolation from Human Blood and Labeling with Multimodal Superparamagnetic Iron Oxide Nanoparticles

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