Update on endoprosthesis pathology: Particle algorithm for particle identification in the SLIM

Krenn, Veit; Kretzer, Jan Philippe; Thomas, Peter A.; Thomsen, M.; Usbeck, Sylvia; Scheuber, L.; Boettner, Friedrich; Rüther, W.; Schulz, Stephan; Zustin, Jozef; Huber, Monika

doi:10.1053/j.sart.2014.01.013

Cited by 11 publications

(20 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Abriebinduzierte Nekrosen (sog. aseptische Nekrosen) können daher histopathologisch die Differentialdiagnose einer mykobakteriellen Infektion in der SLIM darstellen [9,10,14]. Die Tuberkulose erfährt auch in der Orthopädie/Unfallchirurgie insbesondere durch multiresistente Mykobakterien und durch immunmodulierende Therapeutika eine erneute Bedeutung.…”

Section: Tuberkulose (Mykobakterielle Infektion)unclassified

See 1 more Smart Citation

Revisionsendoprothetik

et al. 2015

View full text Add to dashboard Cite

Neutrophil granulocytes can be detected through histochemical methods and more specifically by immune-histopathological techniques and by various quantification systems (histopathological scores) leading to the diagnosis of bacterial peri-implant infection. One important function of histopathology, apart from diagnosing infection, is to rule out other mechanisms of implant failure, such as tumor infiltrations, particle-induced reactions, and adverse reactions to implant materials.

show abstract

Section: Tuberkulose (Mykobakterielle Infektion)unclassified

“…400:1 ) in der Analyse von 10 HPF ("high power field", Sehfeldzahl). Eine aktuelle Studie Fink et al [5] Gontarewicz et al [6] Morawietz et al [15] Partikelcharakterisierung gemäß des Partikelalgorithmus Kölbel et al [8] Krenn et al [9,10] NG neutrophile Granulozyten.…”

Section: Bakterielle Low-grade-infektionunclassified

Revisionsendoprothetik

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Nowadays conventional transmitted light microscopy technique is useful and simple method to determine particle size, shape and structure [10,11]. A highly sensitive optical coherent microscopy (hs-OCM) based on objective-type internal reflection regarding wavelength-modulation may sufficiently improve RBC-EV determination.…”

mentioning

confidence: 99%

Biosensing of red blood cell-derived extracellular vesicles with the advanced bright-field light optical polarization microscopy

Berezin¹,

Mokhnach²,

Byalik³

2017

IJBB

View full text Add to dashboard Cite

Abstract:Background: Red blood cell (RBC)-derived extracellular vesicles (EVs) are recognized a sensitive predictive biomarker of cardiovascular risk, which allow distinguishing vulnerable population from healthy and also physiological aging from premature one. Current known methods of determination of RBC-EVs are based on several principles including flow cytometry, culture methods and various visualization techniques (surface plasmon resonance and computer tomography / magnetic resonance imaging), and various types of microscopy, i.g. high sensitive optical coherent microscopy (hs-OCM), atomic microscopy, fluorescent microscopy. However, there are some limitations and broad variabilities in cost of mentioned above methods of EV-determination. The aim of the study was to compare a capture ability of conventional hs-OCM and advanced bright-field light optical polarization microscopy in detection and measurement of the RBC-EVs. Methods: The study was retrospectively evolved 26 patients with established stable coronary artery disease who were examined between May 2015 and November 2016. The samples of whole blood were collected before ingestion of the meal at room temperature at the morning with powder-free gloves. We used a conventional hs-OCM and advanced bright-field light optical polarization microscopy with improved capture features. Conventional hs-OCM and advanced bright-field light optical polarization microscopy was performed with an Olympus® BH-2 microscope (Olympus, Japan). The whole sample was scanned with the Zeiss 10x objective (100x). The monochromatic laser was used to emanation of light with an appropriate wavelength. It has been identified number and relative size distribution of RBC-EVs with further analysis of morphology using original soft. Results: hs-OCM images supported by yellow-green light allow visualizing cell-free EVs without possibilities for assay their structure and measurement of their number. In contrast, ultraviolet light-enhanced hs-OCM is able to improve capture features of RBC-EVs including their number, diameter and roughly structure. Using advanced bright-field light optical polarization microscopy associated with original soft allows distinguishing low-contrasted objects in details when we used monochromatic light with λ=370+30 nm with further math modelling. Conclusion: the advanced bright-field light optical polarization microscopy allows detecting clinically relevant properties of EV in wide ranges and could be determined a new much promising technique, which allows assaying EV in low cost

show abstract

“…periprothetischen Membran, erfolgt gemäß der SLIM-Konsensusklassifikation [18,23], die gelenkprothesenassoziierte Erkrankungen basierend auf histopathologischen und immunhistochemischen Kriterien einteilt (. Abb.…”

unclassified

“…Der Identifikation von partikulären Prothesenmaterialbestandteilen (Partikeln) in der SLIM und deren Differenzialdiagnose zu partikulären Nicht-Prothesenmaterialbestandteilen kommt in der Abklärung des Gelenkendoprothesenversagens eine wichtige Bedeutung zu [12-14, 20, 22, 23, 26-28]. Diese histopathologische Partikelcharakterisierung kann mithilfe des standardisierten Partikelalgorithmus erfolgen [18,16]. In Verbindung mit biomechanischen, allergologischen, mikrobiologischen, bildgebenden und klinischen Daten ermög-licht dies eine ursächliche Abklärung der Gelenkprothesendysfunktion und des Implantatversagens [1,11,12,28].…”

unclassified

Supramakropartikuläres Polyethylen bei Entzündungen periprothetischer Membranen

et al. 2016

View full text Add to dashboard Cite

The cause and existence of these supra-macroparticulate PE particles (more than 100 µm) is still unclear. A mechanical malfunction seems probable and should be discussed. In prostheses with short lives the proof of supra-macroparticulate PE in SLIM could be a sign of an early mechanical problem. In the wider histopathological particle algorithm supra-macroparticulate PE was considered to fall in the category of macroparticles and should be considered in the histopathological diagnosis of implant failure.

show abstract

Update on endoprosthesis pathology: Particle algorithm for particle identification in the SLIM

Cited by 11 publications

References 62 publications

Revisionsendoprothetik

Revisionsendoprothetik

Biosensing of red blood cell-derived extracellular vesicles with the advanced bright-field light optical polarization microscopy

Supramakropartikuläres Polyethylen bei Entzündungen periprothetischer Membranen

Contact Info

Product

Resources

About