Update on Chagas' disease in Mexico

Dumonteil, Eric

doi:10.1590/s0036-36341999000400010

Cited by 62 publications

(42 citation statements)

References 30 publications

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“…The disease was seen as an exotic ailment and did not receive adequate government attention. The prevalence of Chagas disease in the country ranges from 1.6% to 5.8%, with positivity rates of 0.4% to 19% in different states, 0.17% to 17% in blood banks [4][5][6] . and from 5% to 21.5% in rural areas 6 .…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model

Gómez-Hernández

Rezende-Oliveira

Nascentes

et al. 2011

Rev. Soc. Bras. Med. Trop.

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Introduction: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. Methods: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD -(random amplified polymorphic DNA). Results: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10 6 to 10 7 parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. Conclusions: The Mexican T. cruzi strains are myotrophic and belong to group TcI. Keywords: Trypanosoma cruzi. Mexico. Biological characterization. Genetic characterization. RESUMOIntrodução: Durante muito tempo, foi questionada a importância da doença de Chagas no México onde muitos a consideravam um padecimento exótico. Considerando a grande diversidade genética existente, entre os isolados de Trypanosoma cruzi, a importância da caracterização biológica desses e o escasso número de informações sobre os aspectos clínicos e biológicos da doença de Chagas no México, o objetivo deste trabalho foi realizar a caracterização biológica e molecular de isolados de Trypanosoma cruzi originários de diferentes áreas endêmicas deste país, principalmente do Estado de Jalisco. Métodos: Oito cepas mexicanas de Trypanosoma cruzi foram caracterizadas biologicamente e geneticamente (PCR específica para Trypanosoma cruzi, PCR-multiplex, amplificação do espaço não transcrito dos genes do mini-exon, amplificação das regiões polimórficas do gene do mini-exon, classificação pela amplificação de regiões intergênicas dos genes do spliced leader, RAPD -random amplified polymorphic DNA). Resultados: Foram observados dois tipos de parasitemia: patente com picos máximos de parasitemia entre 4,6x10 6 e 10 7 parasitas/mL e subpatente. Além disso, todos os isolados foram capazes de infectar 100% dos animais. Observou-se tropismo predominante pelo músculo estriado (cardíaco e esquelético). As técnicas de PCR do gene do mini-éxon classificaram as oito cepas como TcI e a técnica de RAPD mostrou variaçã...

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Section: Introductionmentioning

confidence: 99%

Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model

Gómez-Hernández

Rezende-Oliveira

Nascentes

et al. 2011

Rev. Soc. Bras. Med. Trop.

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“…By this we can assume that there are around 1,600,000 people who are carriers of this parasite infection, even though the recognition of clinical cases barely reaches 441 cases in the entire country (Dumonteil 1999). On the other hand, the incidence of this disease has scarcely been explored in some areas of the country, such as Jalisco where it reaches up to 2/1000 people annually (Contreras et al 2000).…”

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confidence: 99%

“…This situation has conditioned the sanitary authorities to minimize the need to implement expensive control measures for a relatively insignificant health problem. However, it is possible that a significant amount of patients with Chagas disease are actually "masked" among patients with heart disease (in the chronic phase) or with a fever of uncertain origin (in the acute phase), and are not registered by the health systems, due to a lack of clinical suspicion, or lack of adequate diagnostic resources in areas infested by triatominae (Dumonteil 1999, Ramsey & Schofield 2003.…”

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confidence: 99%

Active transmission of human chagas disease in Colima Mexico

Coll-Cárdenas

Espinoza‐Gómez

Maldonado-Rodríguez

et al. 2004

Mem. Inst. Oswaldo Cruz

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“…Chagas disease, or American trypanosomiasis, is caused by the protozoan parasite Trypanosoma cruzi, and it represents a major public health problem in Latin America, including Mexico (Dumonteil 1999). Because of difficulties associated with therapeutic treatment, and the elusive development of an effective vaccine, control of the transmission of Chagas disease remains based on vector control by insecticides (Silveira & Vinhaes 1999) and on blood bank screening (Schmuñis 1999).…”

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confidence: 99%