Update on Cardiac Imaging Techniques 2014

The objective of this study is to characterize arterial tissue with and without atherosclerosis by fluorescence lifetime imaging microscopy (FLIM) using Europium Chlortetracycline complex (EuCTc) as fluorescent marker. For this study, twelve rabbits were randomly divided into a control group (CG) and an experimental group (EG), where they were fed a normal and hypercholesterolemic diet, respectively, and were treated for 60 days. Cryosections of the aortic arch specimens were cut in a vertical plane, mounted on glass slides, and stained with Europium (Eu), Chlortetracycline (CTc), Europium Chlortetracycline (EuCTc), and Europium Chlortetracycline Magnesium (EuCTcMg) solutions. FLIM images were obtained with excitation at 405 nm. The average autofluorescence lifetime within plaque depositions was ~1.36 ns. Reduced plaque autofluorescence lifetimes of 0.23 and 0.31 ns were observed on incubation with EuCTc and EuCTcMg respectively. It was observed a quenching of collagen, cholesterol and TG emission spectra increasing EuCTc concentration. The drastic reduction in fluorescence lifetimes is due to a resonant energy transfer between collagen, triglycerides, cholesterol and europium complexes, quenching fluorescence.

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Fluorescent lifetime imaging microscopy using Europium complexes improves atherosclerotic plaques discrimination

Sicchieri¹,

Natal

Courrol³

2016

Int J Cardiovasc Imaging

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Dual Energy Coronary Computed Tomography Angiography for Detection and Quantification of Atherosclerotic Burden: Diagnostic and Prognostic Significance

Andreini

2016

Revista Española de Cardiología (English Edition)

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Angiografía coronaria por tomografía computarizada de doble energía para la detección y la cuantificación de la carga aterosclerótica: importancia diagnóstica y pronóstica

Andreini

2016

Revista Española de Cardiología

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Update on Cardiac Imaging Techniques 2014

Cited by 3 publications

References 83 publications

Fluorescent lifetime imaging microscopy using Europium complexes improves atherosclerotic plaques discrimination

Fluorescent lifetime imaging microscopy using Europium complexes improves atherosclerotic plaques discrimination

Dual Energy Coronary Computed Tomography Angiography for Detection and Quantification of Atherosclerotic Burden: Diagnostic and Prognostic Significance

Angiografía coronaria por tomografía computarizada de doble energía para la detección y la cuantificación de la carga aterosclerótica: importancia diagnóstica y pronóstica

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