Update and Revalidation of Ghose’s Cellulase Assay Methodology

Siqueira, Joyce Gueiros Wanderley; Teixeira, Natascha Amalio; Vandenberghe, Luciana Porto de Souza; Oliveira, Priscilla Zwiercheczewski de; Soccol, Carlos Ricardo; Rodrigues, Cristine

doi:10.1007/s12010-020-03291-0

Cited by 3 publications

(1 citation statement)

References 18 publications

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“…Enzymatic cocktails were purchased from Sigma-Aldrich ® (Pozna ń, Poland). Cellulase activity, expressed as filter paper unit (FPU) per gram of enzyme solution, was determined by the Ghose method, established by the International Union of Pure and Applied Chemistry (IUPAC) [17].…”

Section: Enzymatic Hydrolysismentioning

confidence: 99%

Succinic Production from Source-Separated Kitchen Biowaste in a Biorefinery Concept: Focusing on Alternative Carbon Dioxide Source for Fermentation Processes

Kuglarz

Angelidaki

2023

Fermentation

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This study presents sustainable succinic acid production from the organic fraction of household kitchen wastes, i.e., the organic fraction of household kitchen waste (OFHKW), pretreated with enzymatic hydrolysis (100% cocktail dosage: 62.5% Cellic® CTec2, 31%% β-Glucanase and 6.5% Cellic ® HTec2, cellulase activity of 12.5 FPU/g-glucan). For fermentation, A. succinogenes was used, which consumes CO2 during the process. OFHKW at biomass loading > 20% (dry matter) resulted in a final concentration of fermentable sugars 81–85 g/L and can be treated as a promising feedstock for succinic production. Obtained results state that simultaneous addition of gaseous CO2 and MgCO3 (>20 g/dm3) resulted in the highest sugar conversion (79–81%) and succinic yields (74–75%). Additionally, CH4 content in biogas, used as a CO2 source, increased by 21–22% and reached 91–92% vol. Liquid fraction of source-separated kitchen biowaste and the residue after succinic fermentation were successfully converted into biogas. Results obtained in this study clearly document the possibility of integrated valuable compounds (succinic acid) and energy (biogas) production from the organic fraction of household kitchen wastes (OFHKW).

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Section: Enzymatic Hydrolysismentioning

confidence: 99%