Upconversion fluorescence resonance energy transfer biosensor for sensitive detection of human immunodeficiency virus antibodies in human serum

Wu, Yanmei; Yao, Chuang; Huang, Li-Jiao; Yu, Ru‐Qin; Chu, Xia

doi:10.1039/c4cc00569d

Cited by 79 publications

(41 citation statements)

References 24 publications

(4 reference statements)

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“…Recently, trivalent rare-earth (RE 3+ ) ions doped fluoride nanoparticles (NPs) have been applied widely in many fields of high technology, such as bioimaging, drug delivery, photodynamic therapy, solar cells [1,2,3,4,5,6,7,8,9,10,11,12], etc. In particular, Er 3+ -doped fluoride NPs have been applied in waveguide amplifiers [13,14,15,16] since intra-4f-shell transitions of Er 3+ ions not only cause visible light emissions but also send an emission at 1.5 μm (the 4 I 13/2 → 4 I 15/2 transition of Er 3+ ions), which is located in low loss windows of optical communication networks.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Small Ce3+-Er3+-Yb3+ Tri-Doped BaLuF5 Active-Core-Active-Shell-Active-Shell Nanoparticles with Strong Down Conversion Luminescence at 1.5 μm

et al. 2018

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Small fluoride nanoparticles (NPs) with strong down-conversion (DC) luminescence at 1.5 μm are quite desirable for optical fiber communication systems. Nevertheless, a problem exists regarding how to synthesize small fluoride NPs with strong DC emission at 1.5 μm. Herein, we propose an approach to improve 1.5 μm emission of BaLuF5:Yb3+,Er3+ NPs by way of combining doping Ce3+ ions and coating multiple BaLuF5: Yb3+ active-shells. We prepared the BaLuF5:18%Yb3+,2%Er3+,2%Ce3+ NPs through a high-boiling solvent method. The effect of Ce3+ concentration on the DC luminescence was systematically investigated in the BaLuF5:Yb3+,Er3+ NPs. Under a 980 nm laser excitation, the intensities of 1.53 μm emission of BaLuF5:18%Yb3+,2%Er3+,2%Ce3+ NPs was enhanced by 2.6 times comparing to that of BaLuF5:18%Yb3+,2%Er3+ NPs since the energy transfer between Er3+ and Ce3+ ions: Er3+:4I11/2 (Er3+) + 2F5/2 (Ce3+) → 4I13/2 (Er3+) + 2F7/2 (Ce3+). Then, we synthesized BaLuF5:18%Yb3+,2%Er3+,2%Ce3+@BaLuF5:5%Yb3+@BaLuF5:5%Yb3+ core-active-shell-active-shell NPs via a layer-by-layer strategy. After coating two BaLuF5:Yb3+ active-shell around BaLuF5:Yb3+,Er3+,Ce3+ NPs, the intensities of the 1.53 μm emission was enhanced by 44 times compared to that of BaLuF5:Yb3+,Er3+ core NPs, since the active-shells could be used to not only suppress surface quenching but also to transfer the pump light to the core region efficiently through Yb3+ ions inside the active-shells.

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Section: Introductionmentioning

confidence: 99%

Synthesis of Small Ce3+-Er3+-Yb3+ Tri-Doped BaLuF5 Active-Core-Active-Shell-Active-Shell Nanoparticles with Strong Down Conversion Luminescence at 1.5 μm

et al. 2018

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“…Since the first demonstration of UCL bioassay in 1999 [182], there has been an extensive research on Ln 3+ -doped UCNPs in various bioassay applications [183][184][185][186]. One representative paradigm was reported by Tanke and coworkers [187], who employed Y 2 O 2 S : Yb,Er submicrons as UCL probes in DNA microarrays for the detection of nucleic acid hybrids.…”

Section: Upconverting Luminescent Bioassaymentioning

confidence: 99%

“…For the detection of human immunodeficiency virus (HIV) antibody, Chu and coworkers [185] designed an UC-FRET biosensor by using the peptide-functionalized NaYF 4 : Yb,Er UCNPs as energy donors and GO as energy quenchers. The UCNP donors were initially adsorbed on the surface of GO via π-π stacking interactions and hydrophobic interactions between the peptides and GO, which resulted in a complete UCL quenching of the donor through energy transfer or electron transfer processes.…”

Section: Upconverting Luminescent Bioassaymentioning

confidence: 99%

Inorganic lanthanide nanoprobes for background-free luminescent bioassays

Huang

Zheng

et al. 2015

Sci. China Mater.

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Luminescent bioassay techniques have been widely adopted in a variety of research and medical institutions. However, conventional luminescent bioassays utilizing traditional bioprobes like organic dyes and quantum dots often suffer from the interference of background noise from scattered lights and autofluorescence from biological matrices. To eliminate this disadvantage, the use of inorganic lanthanide (Ln 3+ )-doped nanoparticles (NPs) is an excellent option in view of their superior optical properties, such as the long-lived downshifting luminescence, near-infrared triggered anti-Stokes upconverting luminescence and excitation-free persistent luminescence. In this review, we summarize the latest advances in the development of inorganic Ln 3+ -doped NPs as sensitive luminescent bioprobes from their fundamental physicochemical properties to biodetection, including the chemical synthesis, surface functionalization, optical properties and their promising applications for background-free luminescent bioassays. Future efforts and prospects towards this rapidly growing field are also proposed. INTRODUCTIONSensitive and specific bioassay of trace amount of target analytes is essential for a variety of biomedical applications ranging from pharmaceutical preparation to disease diagnosis and therapy [1][2][3]. Among various bioassay methods, luminescent bioassay has received particular attention because of its high sensitivity and good specificity [4,5]. Conventional luminescent bioassay techniques such as enzyme-linked immunosorbent assay (ELISA), time-resolved (TR) fluoroimmunoassay (TRFIA), Förster resonance energy transfer (FRET) and TR-FRET assays have laid the foundation for many modern clinical applications [6][7][8][9][10][11]. However, these bioassays are impeded by the availability of traditional bioprobes like organic dyes, lanthanide (Ln 3+ ) chelates and quantum dots (QDs). The use of these bioprobes has a number of limitations. Organic dyes commonly possess poor photochemical stability and suffer from serious photobleaching [12]. The applicability of QDs is compromised by photoblinking and high toxic- ity of heavy metal elements like cadmium and selenium, especially for in vivo biosensing [13,14]. Moreover, both organic dyes and QDs may induce high background noise and considerable photodamage to the biological samples under ultraviolet (UV) excitation, which deteriorates their detection sensitivity for bioassays [15,16]. Although such background noise can be suppressed by the technique of TR photoluminescence (PL) through the use of the longlived luminescence of Ln 3+ -chelates, the poor photochemical stability, long-term toxicity and high cost of Ln 3+ -chelates remain a major issue [17]. These concerns fuel a crucial demand for the development of a new generation of luminescent bioprobes to circumvent the limitations of traditional ones.Recently, with the explosion of nanoscience and nanotechnology, there is a growing dedication towards the development of diverse luminescent nanoprobes for biodetection ...

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“…reported fluorescent boronate-modified polyacrylonitrile nanoparticles of 50 nm diameter were fabricated for use as a selective H 2 O 2 sensor25. Wu and co-workers proposed to prepare hydrophilic and peptide-functionalized upconversion fluorescent nanoparticles, which were used in the design of a biosensor for the sensitive and selective determination of human immunodeficiency virus antibodies in human serum26. In our group, Gao and co-workers have prepared one molecularly imprinted polymer microspheres for ultra-trace nonfluorescent cyhalothrin in honey27.…”

mentioning

confidence: 99%

A Novel Sensitive Luminescence Probe Microspheres for Rapid and Efficient Detection of τ-Fluvalinate in Taihu Lake

Wang

Qiu

et al. 2017

Sci Rep

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Fluorescent molecularly imprinted polymers have shown great promise in biological or chemical separations and detection, due to their high stability, selectivity and sensitivity. In this work, fluorescent molecularly imprinted microsphere was synthesized via precipitation polymerization, which could separate efficiently and rapidly detect τ-fluvalinate (a toxic insecticide) in water samples, was reported. The fluorescent imprinted sensor showed excellent stability, outstanding selectivity and the limit of detection low to 12.14 nM, good regeneration ability which still kept good sensitivity after 8 cycling experiments and fluorescence quenching mechanism was illustrated in details. In addition, the fluorescent sensor was further used to detect τ-fluvalinate in real samples from Taihu Lake. Despite the relatively complex components of the environment water, the fluorescent imprinted microspheres sitll showed good recovery, clearly demonstrating the potental value of this smart sensor nanomaterial in environment monitoring.

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Upconversion fluorescence resonance energy transfer biosensor for sensitive detection of human immunodeficiency virus antibodies in human serum

Cited by 79 publications

References 24 publications

Synthesis of Small Ce3+-Er3+-Yb3+ Tri-Doped BaLuF5 Active-Core-Active-Shell-Active-Shell Nanoparticles with Strong Down Conversion Luminescence at 1.5 μm

Synthesis of Small Ce3+-Er3+-Yb3+ Tri-Doped BaLuF5 Active-Core-Active-Shell-Active-Shell Nanoparticles with Strong Down Conversion Luminescence at 1.5 μm

Inorganic lanthanide nanoprobes for background-free luminescent bioassays

A Novel Sensitive Luminescence Probe Microspheres for Rapid and Efficient Detection of τ-Fluvalinate in Taihu Lake

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