Up-to-Date Applications of Microarrays and Their Way to Commercialization

Schumacher, Sarah; Muekusch, Sandra; Seitz, Harald

doi:10.3390/microarrays4020196

Cited by 13 publications

(4 citation statements)

References 68 publications

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“…The microarray/chip-based technology provides superior sensitivity; minimum sample/reagent requirement; and, most importantly, the preprinted target-capture module and ready-to-use interface for reaction. Microarrays have been widely applied in basic biological research and clinical diagnosis, such as protein interaction studies [34], immune profiling [35], vaccine development [36], biomarker discovery [37], disease diagnostics [38], and drug screening and discovery [39], among others. Microarray technologies have been used for mycotoxin detection due to their sensitivity [40].…”

Section: Discussionmentioning

confidence: 99%

An Aptamer-Array-Based Sample-to-Answer Biosensor for Ochratoxin A Detection via Fluorescence Resonance Energy Transfer

Peng²,

et al. 2021

Chemosensors

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Food toxins are a hidden threat that can cause cancer and tremendously impact human health. Therefore, the detection of food toxins in a timely manner with high sensitivity is of paramount importance for public health and food safety. However, the current detection methods are relatively time-consuming and not practical for field tests. In the present work, we developed a novel aptamer-chip-based sample-to-answer biosensor (ACSB) for ochratoxin A (OTA) detection via fluorescence resonance energy transfer (FRET). In this system, a cyanine 3 (Cy3)-labeled OTA-specific biotinylated aptamer was immobilized on an epoxy-coated chip via streptavidin-biotin binding. A complementary DNA strand to OTA aptamer at the 3′-end was labeled with a black hole quencher 2 (BHQ2) to quench Cy3 fluorescence when in proximity. In the presence of OTA, the Cy3-labeled OTA aptamer bound specifically to OTA and led to the physical separation of Cy3 and BHQ2, which resulted in an increase of fluorescence signal. The limit of detection (LOD) of this ACSB for OTA was 0.005 ng/mL with a linearity range of 0.01–10 ng/mL. The cross-reactivity of ACSB against other mycotoxins, ochratoxin B (OTB), aflatoxin B1 (AFB1), zearalenone (ZEA), or deoxynilvalenol (DON), was less than 0.01%. In addition, this system could accurately detect OTA in rice samples spiked with OTA, and the mean recovery rate of the spiked-in OTA reached 91%, with a coefficient of variation (CV) of 8.57–9.89%. Collectively, the ACSB may represent a rapid, accurate, and easy-to-use platform for OTA detection with high sensitivity and specificity.

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Section: Discussionmentioning

confidence: 99%

An Aptamer-Array-Based Sample-to-Answer Biosensor for Ochratoxin A Detection via Fluorescence Resonance Energy Transfer

Peng²,

et al. 2021

Chemosensors

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“…Another challenging field for protein array is related to rheumatoid arthritis (RA) as it could detect biomarkers specific for arthritis and not for autoimmune diseases in general [100]. Some research groups have started to develop different antigen arrays for differential diagnosis and even RA molecular classifying.…”

Section: Immunoassay Methods In Immunogenetic Disease Diagnosticmentioning

confidence: 99%

Immunoassay Techniques Highlighting Biomarkers in Immunogenetic Diseases

Manole¹,

Bastian²,

Popescu³

et al. 2019

Immunogenetics

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Diagnosis of autoimmune diseases is crucial for the clinician and the patient alike. The immunoassay techniques most commonly used for this purpose are immunohistochemistry, ELISA, and Western blotting. For the detection of more specific biomarkers or the discovery of new ones for diagnostic purposes and as therapeutic targets, microarray techniques are increasingly used, for example, protein microarray, Luminex, and in recent years, surface plasmon resonance imaging. All of these technologies have undergone changes over time, making them easier to use. Similar technologies have been invented but responding to specific requirements for both diagnostic and research purposes. The goals are to study more analytes in the same sample, in a shorter time, and with increased accuracy. The reproducibility and reliability of the results are also a target pursued by manufacturers. In this chapter, we present these technologies and their utility in the diagnosis of immunogenetic diseases.

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“…However, the detection of false-positive results could increase, too. The sensitivity of the bioanalytical test could also be increased by using alternative markers, for example based on fluorescence or magneto resistive signal [6], [7], [9] - [11]. The other component of the LFA that could influence the sensitivity is the material on the backing card: nitrocellulose membrane.…”

Section: Summary and Perspectivementioning

confidence: 99%

Development of a Smartphone Based Reader for the Quantitative Analysis of Lateral Flow Assays

Schneider

Selig²,

Keil³

et al. 2018

MSF

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Smartphones are developing into all-purposes devices. In the present work, the employment/application of smartphones as medical devices in home care and point-of-care (POC) diagnostics are investigated in the analysis of Lateral Flow Assays (LFA). A smartphone-based LFA reader was developed for the quantitative analysis of D-Dimer – a biomarker indicating e.g. thrombotic event or danger of embolism.The proof-of-concept has been shown with multiple smartphones in establishing: (I) Optimal dimensions of the LFA cell of 72.11mm distance of smartphone to D-Dimer test leading to a coefficients of variances (CV) between 0.8% and 4.2%. (II) Inter-device investigations: CVs around 13.5%; a limit of detection (LOD) of 100ng/ml (DDU) D-Dimer. (III) Inter-smartphone investigations: CV about 16%, a limit of detection (LOD) at 66.4ng/ml (DDU). (IV) Calibrations: CV and LOD of three smartphones are comparable to the commercial available LFA reader. Further development to put the multiple smartphone-based LFA reader on the market.

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Up-to-Date Applications of Microarrays and Their Way to Commercialization

Cited by 13 publications

References 68 publications

An Aptamer-Array-Based Sample-to-Answer Biosensor for Ochratoxin A Detection via Fluorescence Resonance Energy Transfer

An Aptamer-Array-Based Sample-to-Answer Biosensor for Ochratoxin A Detection via Fluorescence Resonance Energy Transfer

Immunoassay Techniques Highlighting Biomarkers in Immunogenetic Diseases

Development of a Smartphone Based Reader for the Quantitative Analysis of Lateral Flow Assays

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