Up-regulation of transcriptional factor E2F1 in papillary and anaplastic thyroid cancers

Onda, Masamitsu; Nagai, Hisaki; Yoshida, Akira; Miyamoto, Shizuyo; Asaka, Shinichi; Akaishi, Junko; Takatsu, Keisuke; Nagahama, Mitsuji; Ito, Kouichi; Shimizu, Kazuo; Emi, Mitsuru

doi:10.1007/s10038-004-0146-3

Cited by 39 publications

(27 citation statements)

References 34 publications

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“…Every cancer-related defect in these pathways should result in p14 ARF upregulation. We found an upregulation of E2F1 in all tumour types which is consistent with others studies (Saiz et al, 2002;Onda et al, 2004), and we observed a link between E2F1 and p14 ARF expression in nontumoral thyroid tissue as well as in follicular adenomas and carcinomas. These results suggest the involvement of E2F1 in the p14 ARF upregulation in these tumours.…”

Section: Discussionsupporting

confidence: 93%

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The status of CDKN2A alpha (p16INK4A) and beta (p14ARF) transcripts in thyroid tumour progression

Ferru

Fromont

Gibelin³

et al. 2006

Br J Cancer

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CDKN2A locus on chromosome 9p21 encodes two tumour suppressor proteins pl6 INK4A , which is a regulator of the retinoblastoma (RB) protein, and p14 ARF , which is involved in the ARF -Mdm2 -p53 pathway. The aim of this study was to determine if CDKN2A gene products are implicated in differentiated thyroid carcinogenesis and progression. We used real-time quantitative RT -PCR and immunohistochemistry to assess both transcripts and proteins levels in 60 tumours specimens. Overexpression of p14 ARF and pl6 INK4A was observed in follicular adenomas, follicular carcinomas and papillary carcinomas, while downregulation was found in oncocytic adenomas compared to nontumoral paired thyroid tissues. These deregulations were statistically significant for pl6 INK4a (P ¼ 0.006) in follicular adenomas and close to statistical significance for p14 ARF in follicular adenomas (P ¼ 0.06) and in papillary carcinomas (P ¼ 0.05). In all histological types, except papillary carcinomas, we observed a statistically significant relationship between p14 ARF and E2F1 (r ¼ 0.64 to 1, Po0.05). Our data are consistent with involvement of CDKN2A transcript upregulation in thyroid follicular tumorigenesis as an early event. However, these deregulations do not appear to be correlated to the clinical outcome and they could not be used as potential prognostic markers.

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Section: Discussionsupporting

confidence: 93%

“…For this we determined first the mRNA expression levels of E2F1 in the same tumours. Consistent with others studies, E2F1 levels were upregulated in all tumour types (Onda et al, 2004). This upregulation was statistically significant in PTC (P ¼ 0.0001) ( (Bates et al, 1998).…”

Section: P14 Arf Regulation By E2f1 Is Lost In Papillary Carcinomassupporting

confidence: 91%

The status of CDKN2A alpha (p16INK4A) and beta (p14ARF) transcripts in thyroid tumour progression

Ferru

Fromont

Gibelin³

et al. 2006

Br J Cancer

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“…After 72 hours, cells were collected and extracted RNA with TRIzol (Invitrogen). 7 The expression of OEATC-1 was evaluated with SQ-PCR in the manner described above.…”

Section: Transfection Of Psec Oeatc-1 To the Kta2 Cell Line And Gene mentioning

confidence: 99%

Overexpressed in anaplastic thyroid carcinoma‐1 (OEATC‐1) as a novel gene responsible for anaplastic thyroid carcinoma

Mizutani

Onda

Asaka

et al. 2005

Cancer

Self Cite

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BACKGROUNDAnaplastic thyroid carcinoma (ATC) is one of the most fulminant human malignancies. However, the molecular carcinogenic mechanisms of ATC are understood poorly. Recently, the authors performed a cyclic DNA (cDNA) microarray analysis with 11 anaplastic thyroid carcinoma cell lines (ACLs) and discovered several novel responsible genes for ACLs and ATC. From the extended list, they focused on hypothetical and anonymous genes and investigated a novel gene, named the overexpressed in anaplastic thyroid carcinoma‐1 (OEATC‐1) gene.METHODSTo investigate the role of the OEATC‐1 gene in ATC carcinogenesis, first, the expression levels of OEATC‐1 in ACLs, in various types of carcinoma cell lines, and in normal human tissues were examined with reverse transcriptase‐polymerase chain reaction analysis. To explore the effect of OEATC‐1 in ATC development, a cell‐growth assay was performed with KTA2 cells under OEATC‐1 gene silencing using small‐interfering RNA (siRNA).RESULTSOEATC‐1 was overexpressed significantly in ACLs and in other types of carcinoma cell lines with various expression levels. Conversely, in normal human tissues, OEATC‐1 was expressed weakly in placenta, kidney, spleen, thymus, small intestine, and thyroid gland. To evaluate the effects of OEATC‐1 on tumor cell growth, gene silencing was caused by transfecting the plasmid‐generating siRNA effect to KTA2 cells. Consequently, the silencing of OEATC‐1 significantly suppressed the cell growth compared with controls.CONCLUSIONSThe current results indicated that OEATC‐1 may have some oncogenic or cell growth‐promoting function in ACL. OEATC‐1 is considered a novel responsible gene in ATC. Cancer 2005. © 2005 American Cancer Society.

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“…The complex was transfected into arranged KTA2 and it was cultured up to 7 days. At the determined time points of days 0, 3, 5 and 7, cells were collected and RNA was extracted with TRIzol (Onda et al, 2004b). The exogenous expression of HBB was confirmed with both SQ-PCR and Q-PCR in the same manner as described previously.…”

Section: Construction Of Hbb Expression Vectormentioning

confidence: 99%

“…Q-PCR was carried with qPCR Mastermix for Syber Green I (Eurogenetec, Seraing, Belgium) and ABI 7700 (Applied Biosystems, Foster City, CA, USA) described previously (Onda et al, 2004b). Primers for Q-PCR of HBB were the same as those used in the SQ-PCR study.…”

Section: Quantitative Rt -Pcrmentioning

confidence: 99%

Decreased expression of haemoglobin beta (HBB) gene in anaplastic thyroid cancer and recovory of its expression inhibits cell growth

et al. 2005

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Anaplastic thyroid cancer (ATC) is one of the most fulminant and foetal diseases in human malignancies. However, the genetic alterations and carcinogenic mechanisms of ATC are still unclear. Recently, we investigated the gene expression profile of 11 anaplastic thyroid cancer cell lines (ACL) and significant decreased expression of haemoglobin beta (HBB) gene in ACL. Haemoglobin beta is located at 11p15.5, where loss of heterozygosity (LOH) was reported in various kinds of cancers, including ATC, and it has been suggested that novel tumour suppressor genes might exist in this region. In order to clarify the meaning of decreased expression of HBB in ATC, the expression status of HBB was investigated with ACL, ATC, papillary thyroid cancer (PTC) and normal human tissues. Haemoglobin beta showed significant decreased expression in ACLs and ATCs; however, in PTC, HBB expressed equal to the normal thyroid gland. In addition, HBB expressed in normal human tissues ubiquitously. To validate the tumour-suppressor function of HBB, cell growth assay was performed. Forced expression of HBB in KTA2 cell, which is a kind of ACL, significantly suppressed KTA2 growth. The mechanism of downregulation of HBB in ATC is still unclear; however, our results suggested the possibility of HBB as a novel tumour-suppressor gene.

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Up-regulation of transcriptional factor E2F1 in papillary and anaplastic thyroid cancers

Cited by 39 publications

References 34 publications

The status of CDKN2A alpha (p16INK4A) and beta (p14ARF) transcripts in thyroid tumour progression

The status of CDKN2A alpha (p16INK4A) and beta (p14ARF) transcripts in thyroid tumour progression

Overexpressed in anaplastic thyroid carcinoma‐1 (OEATC‐1) as a novel gene responsible for anaplastic thyroid carcinoma

Decreased expression of haemoglobin beta (HBB) gene in anaplastic thyroid cancer and recovory of its expression inhibits cell growth

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