Up-regulation of TDAG51 is a dependent factor of LPS-induced RAW264.7 macrophages proliferation and cell cycle progression

Hu, Jiao; Jia, Xiaoyun; Zhao, Tianjing; Hui, Rutai; Zhang, Jianing; Cheng, Ying; Zhu, Huapei; Xu, Kailian; Guo, Shiyu; Shi, Qi; Zhang, Hui; Wang, Feng-Yang; Chen, Chuangfu; Du, Lijun

doi:10.3109/08923973.2016.1138968

Cited by 11 publications

(12 citation statements)

References 27 publications

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“…However, the PTEN inhibitor SF1670 completely abrogated the effect of coelonin (Figure 8). We noticed that LPS treatment also induced RAW264.7 cells G1 phase arrest (Figure 8B), which contradicts a previous report [20], but is consistent with report [49]. The exact molecular mechanism underlying these contradictory results need to be further studied, but it is clear that LPS-induced G1 phase arrest in RAW264.7 cells did not occur through the up-regulation of p27 Kip1 .…”

Section: Discussionsupporting

confidence: 77%

“…In addition, we performed a protein network analysis using REACTOME () to identify major interactions, three major cellular processes were significantly enriched ( p value < 0.05) as follows: (1) Immune system, (2) signal transduction, and (3) cell cycle. LPS is the component of the outer membrane of Gram-negative bacteria, and is one of the most well characterized pathogen-associated molecular patterns (PAMPs), which can be recognized by Toll-like receptor 4 (TLR4), and trigger innate responses [19], such as enhancing the secretion of cytokines and chemokines, and promoting macrophages migration and proliferation [20]. Therefore, it is evident that the protein network analysis results imply coelonin may block LPS induced RAW264.7 cell signal transduction, immune response, and proliferation.…”

Section: Resultsmentioning

confidence: 99%

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Coelonin, an Anti-Inflammation Active Component of Bletilla striata and Its Potential Mechanism

Jiang

Wang

et al. 2019

IJMS

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Ethanol extract of Bletilla striata has remarkable anti-inflammatory and anti-pulmonary fibrosis activities in the rat silicosis model. However, its active substances and molecular mechanism are still unclear. To uncover the active ingredients and potential molecular mechanism of the Bletilla striata extract, the lipopolysaccharide (LPS)-induced macrophage inflammation model and phospho antibody array were used. Coelonin, a dihydrophenanthrene compound was isolated and identified. It significantly inhibited LPS-induced interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) expression at 2.5 μg/mL. The microarray data indicate that the phosphorylation levels of 32 proteins in the coelonin pre-treated group were significantly down-regulated. In particular, the phosphorylation levels of the key inflammatory regulators factor nuclear factor-kappa B (NF-κB) were significantly reduced, and the negative regulator phosphatase and tensin homologue on chromosome ten (PTEN) was reduced. Moreover, the phosphorylation level of cyclin dependent kinase inhibitor 1B (p27Kip1), another downstream molecule regulated by PTEN was also reduced significantly. Western blot and confocal microscopy results confirmed that coelonin inhibited LPS-induced PTEN phosphorylation in a dose-dependent manner, then inhibited NF-κB activation and p27Kip1 degradation by regulating the phosphatidylinositol-3-kinases/ v-akt murine thymoma viral oncogene homolog (PI3K/AKT) pathway negatively. However, PTEN inhibitor co-treatment analysis indicated that the inhibition of IL-1β, IL-6 and TNF-α expression by coelonin was independent of PTEN, whereas the inhibition of p27Kip1 degradation resulted in cell-cycle arrest in the G1 phase, which was dependent on PTEN. The anti-inflammatory activity of coelonin in vivo, which is one of the main active ingredients of Bletilla striata, deserves further study.

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Section: Discussionsupporting

confidence: 77%

Section: Resultsmentioning

confidence: 99%

Coelonin, an Anti-Inflammation Active Component of Bletilla striata and Its Potential Mechanism

Jiang

Wang

et al. 2019

IJMS

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“…The statistical significance of differences between mean values for the various experimental groups and controls was determined as previously described (Jiao et al, 2016). Values of P <0.05 were considered significantly different (*), and P<0.01 indicated high statistical differences (**).…”

Section: Discussionmentioning

confidence: 99%

“…PAM cells were seeded into 12-well culture plates (8×10 5 cells/well) at 0 h, stimulated by B.suis LPS at the concentrations of 0.1, 1, 10, 100, 1000 μg/mL after 24 h, harvested at 48 h. And then, the concentration of 100 ng/mL B. suis LPS was used for stimulation of the time points of 3, 6, 12, 24, 48 and 96 h. siRNAs transfection: PAM cells were seeded into 12-well culture plates (8×10 5 cells/well) at 0 h, to optimize siRNA transfection efficiency, we used X-tremeGENE siRNA Transfection Reagent (Roche, Basel, Switzerland) to transfect with the FITC-siRNA (Santa Cruz Biotechnology, CA, USA) at 12 h post-seeding, optimizing the ratio of X-tremeGENE siRNA Transfection Reagent and FITC-siRNA obtained a more than 90% transfection efficiency measured by flow cytometry. Caspase-11 siRNA targeting caspase-11 (Santa Cruz Biotechnology) siRNAs or Control siRNA-A (scramble siRNA, Santa Cruz Biotechnology) were introduced into PAM cells as described previously (Jiao et al, 2016), and according to the instructions of X-tremeGENE siRNA Transfection Reagent (Roche, Basel, Switzerland) .…”

Section: Bsuis Lps Extraction and Cell Stimulationmentioning

confidence: 99%

“…The total protein was extracted, western blotting conducted as described previously (Jiao et al, 2016). The primary antibodies included rabbit anti-Swine caspase-11 monoclonal antibody (mAB) (1:1000 dilution; Santa Cruz Biotechnology, USA), and rabbit anti-GAPDH mAb (1:5000 dilution; Abcam, USA) as a control.…”

Section: Protein Extraction and Western Blot Analysismentioning

confidence: 99%

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aspase-11 plays an important role in IL-1, IL-18 and IL-1â secretion from porcine alveolar macrophage cells stimulated with Brucella suis LPS

Zhao

Shuai

et al. 2019

IJAR

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Brucella spp. is the causative agent of brucellosis, an extremely important disease worldwide. Innate immune cells detect pathogens via repeated cellular patterns (PAMPs) such as the Brucella suis (B. suis) lipopolysaccharide (LPS) coat on Gram-negative bacteria, which act as an important virulence factor of Brucella. B.suis LPS may be an issue for pro-inflammatory cytokine induction such as interleukin-1 (IL-1), interleukin-18 (IL-18) and interleukin-1â (IL-1â), which released from immune cells to mediate downstream inflammatory effects. To elucidate the mechanism of how B.suis LPS affects the secretion of IL-1, IL-18 and IL-1â in macrophages. We identified the up-regulation of caspase-11 in a porcine alveolar macrophages (PAM) cells stimulated with B.suis LPS. Furthermore, specific small interfering RNA (siRNA) targeting caspase-11 effectively inhibited B.suis LPS stimulated IL-1, IL-18 and IL-1â release from PAM. The results indicated that the concentrations of IL-1, IL-18 and IL-1â of caspase-11 siRNA pretreated group were lower than that of control significantly. Caspase-11 plays an important role in IL-1, IL-18 and IL-1â secretion from porcine alveolar macrophage cells stimulated with Brucella suis LPS, and these findings might aid our understanding of the pathogenic mechanisms of Brucella and provide an entirely new innate immune response mechanism underlying macrophages dysfunction during Brucella infection.

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TDAG51-Deficiency Podocytes are Protected from High-Glucose-Induced Damage Through Nrf2 Activation via the AKT–GSK-3β Pathway

Liu

Wang

2022

Inflammation

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Up-regulation of TDAG51 is a dependent factor of LPS-induced RAW264.7 macrophages proliferation and cell cycle progression

Cited by 11 publications

References 27 publications

Coelonin, an Anti-Inflammation Active Component of Bletilla striata and Its Potential Mechanism

Coelonin, an Anti-Inflammation Active Component of Bletilla striata and Its Potential Mechanism

aspase-11 plays an important role in IL-1, IL-18 and IL-1â secretion from porcine alveolar macrophage cells stimulated with Brucella suis LPS

TDAG51-Deficiency Podocytes are Protected from High-Glucose-Induced Damage Through Nrf2 Activation via the AKT–GSK-3β Pathway

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