Up-regulation of SGTB is associated with neuronal apoptosis after neuroinflammation induced by lipopolysaccharide

Cao, Mei; Xu, Wei; Yu, Jian; Zheng, Heyi; Tan, Xiang; Li, Lei; Rui, Ying; Xu, Guofeng; Cui, Gang; Xu, Jian; Cao, Jianhua; Tao, Tao; Ke, Kaifu; Wu, Qianhui

doi:10.1007/s10735-013-9517-4

Cited by 12 publications

(8 citation statements)

References 37 publications

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“…5A). SGTB plays a crucial role in mediating cell apoptosis via the caspase-3-dependent pathway29. In this study, we also observed up-regulated expression of SGTB, targeted by miR-133a-3p, miR-181a-5p, miR-409-5p, and miR-542-3p, at both the transcriptional and protein levels in Sertoli cells following exposure to MC-LR (Supplementary Figure S1B and Fig.…”

Section: Resultssupporting

confidence: 69%

Microcystin-Leucine Arginine Causes Cytotoxic Effects in Sertoli Cells Resulting in Reproductive Dysfunction in Male Mice

Chen

Zhou

Wang

et al. 2016

Sci Rep

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Microcystin-leucine arginine (MC-LR) is a potent toxin for Sertoli cells. However, the specific molecular mechanisms of MC-induced cytotoxicity still remain unclear. In this study, we performed a comprehensive analyses of changes of miRNAs and mRNAs in Sertoli cells treated with MC-LR. Through computational approaches, we showed the pivotal roles of differentially expressed miRNAs that were associated with cell metabolism, cellular growth and proliferation, cell-to-cell signaling and interaction and cellular movement. Ingenuity Pathway Analyses (IPA) revealed some differentially expressed miRNAs and mRNAs that may cause reproductive system diseases. Target gene analyses suggested that destruction in tight junctions (TJ) and adherens junctions (AJ) in testes may be mediated by miRNAs. Consistent with a significant enrichment of chemokine signaling pathways, we observed numerous macrophages in the testes of mice following treatment with MC-LR, which may cause testicular inflammation. Moreover, miR-98-5p and miR-758 were predicted to bind the 3′-UTR region of the mitogen-activated protein kinase 11 (MAPK11, p38 β isoform) gene which stimulates tumor necrosis factor-α (TNF-α) expression in Sertoli cells. TNF-α could interact with the tumor necrosis factor receptor 1 (TNFR1) on germ cells leading to induction of germ cell apoptosis. Collectively, our integrated miRNA/mRNA analyses provided a molecular paradigm, which was experimentally validated, for understanding MC-LR-induced cytotoxicity.

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Section: Resultssupporting

confidence: 69%

Microcystin-Leucine Arginine Causes Cytotoxic Effects in Sertoli Cells Resulting in Reproductive Dysfunction in Male Mice

Chen

Zhou

Wang

et al. 2016

Sci Rep

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“…In the present study, miR‐142‐5p is revealed to target SGTB. As reported previously, up‐regulation of SGTB is related to neuronal apoptosis after neuroinflammation . In osteoarthritis, the pro‐apoptotic role of SGTB for chondrocytes has been demonstrated .…”

Section: Discussionsupporting

confidence: 66%

“…As reported previously, up-regulation of SGTB is related to neuronal apoptosis after neuroinflammation. 36 In osteoarthritis, the pro-apoptotic role of SGTB for chondrocytes has been demonstrated. 23 Functional assays in the present study described the antiproliferation and pro-apoptosis role of SGTB.…”

Section: The Regulatory Axis Of Xist/mir-142-5p/sgtb In Il-1β-inducmentioning

confidence: 99%

miR‐142‐5p protects against osteoarthritis through competing with lncRNA XIST

Sun

et al. 2020

The Journal of Gene Medicine

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Background The relevance between abnormal microRNA expression and osteoarthritis (OA) has been elaborated in recent studies. Hence, the present study aimed to assess the impact of miR‐142‐5p on chondrocyte growth and apoptosis. Methods To mimic OA‐like chondrocyte damage, interleukin (IL)‐1β was used for chondrocyte treatment. The expression of miR‐142‐5p, SGTB, long non‐coding RNA (lncRNA) X inactive specific transcript (XIST) and involved molecules such as Col2A1, Bcl‐2, MMP13 and Bax was determined via a quantitative reverse transcriptase‐polymerase chain reaction and western blot analyses. Functional roles of miR‐142‐5p, SGTB and XIST were monitored in 5‐ethynyl‐2'‐deoxyuridine, CCK‐8 and TUNEL experiments. Rescue analyses were conducted to consolidate the effect of the XIST/miR‐142‐5p/SGTB axis on chondrocytes in OA. Results miR‐142‐5p was down‐regulated in IL‐1β‐treated chondrocytes, whereas SGTB and XIST levels were increased. Overexpression of miR‐142‐5p stimulated proliferation and retarded apoptosis in IL‐1β‐treated chondrocytes. Meanwhile, miR‐142‐5p elevation was correlated with an elevation of Col2A1 and Bcl‐2, as well as a decline of MMP13 and Bax. A mechanistic study showed that miR‐142‐5p negatively regulated SGTB expression. Moreover, we found that lncRNA XIST could relieve the inhibition of miR‐142‐5p on SGTB expression. Augmentation of SGTB or suppression of miR‐142‐5p reversed the influence of XIST depletion on chondrocyte growth and apoptosis. Conclusions The present study has explored the fundamental role of miR‐142‐5p in IL‐1β‐treated chondrocytes, as well as the novel molecular mechanism constituted by miR‐142‐5p/SGTB/XIST in OA. Potentially, the results obtained may add new insight into OA pathogenesis.

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“…In CD8+ cells, we identified two significant differentially expressed transcripts at T6 versus controls, SGTB and FCGR3A . Little is known about SGTB but it has been associated with neuronal apoptosis after neuroinflammation [ 18 ]. Interestingly, FCGR3A encodes CD16a, which forms part of the Fc receptor of the immunoglobulin complex and interacts with a number of immune-related proteins including CD4 and PTPRC, a protein required for T-cell activation.…”

Section: Discussionmentioning

confidence: 99%

Longitudinal expression profiling of CD4+ and CD8+ cells in patients with active to quiescent giant cell arteritis

et al. 2018

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BackgroundGiant cell arteritis (GCA) is the most common form of vasculitis affecting elderly people. It is one of the few true ophthalmic emergencies but symptoms and signs are variable thereby making it a challenging disease to diagnose. A temporal artery biopsy is the gold standard to confirm GCA, but there are currently no specific biochemical markers to aid diagnosis. We aimed to identify a less invasive method to confirm the diagnosis of GCA, as well as to ascertain clinically relevant predictive biomarkers by studying the transcriptome of purified peripheral CD4+ and CD8+ T lymphocytes in patients with GCA.MethodsWe recruited 16 patients with histological evidence of GCA at the Royal Victorian Eye and Ear Hospital, Melbourne, Australia, and aimed to collect blood samples at six time points: acute phase, 2–3 weeks, 6–8 weeks, 3 months, 6 months and 12 months after clinical diagnosis. CD4+ and CD8+ T-cells were positively selected at each time point through magnetic-assisted cell sorting. RNA was extracted from all 195 collected samples for subsequent RNA sequencing. The expression profiles of patients were compared to those of 16 age-matched controls.ResultsOver the 12-month study period, polynomial modelling analyses identified 179 and 4 statistically significant transcripts with altered expression profiles (FDR < 0.05) between cases and controls in CD4+ and CD8+ populations, respectively. In CD8+ cells, two transcripts remained differentially expressed after 12 months; SGTB, associated with neuronal apoptosis, and FCGR3A, associatied with Takayasu arteritis. We detected genes that correlate with both symptoms and biochemical markers used for predicting long-term prognosis. 15 genes were shared across 3 phenotypes in CD4 and 16 across CD8 cells. In CD8, IL32 was common to 5 phenotypes including Polymyalgia Rheumatica, bilateral blindness and death within 12 months.ConclusionsThis is the first longitudinal gene expression study undertaken to identify robust transcriptomic biomarkers of GCA. Our results show cell type-specific transcript expression profiles, novel gene-phenotype associations, and uncover important biological pathways for this disease. In the acute phase, the gene-phenotype relationships we have identified could provide insight to potential disease severity and as such guide in initiating appropriate patient management.Electronic supplementary materialThe online version of this article (10.1186/s12920-018-0376-4) contains supplementary material, which is available to authorized users.

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Up-regulation of SGTB is associated with neuronal apoptosis after neuroinflammation induced by lipopolysaccharide

Cited by 12 publications

References 37 publications

Microcystin-Leucine Arginine Causes Cytotoxic Effects in Sertoli Cells Resulting in Reproductive Dysfunction in Male Mice

Microcystin-Leucine Arginine Causes Cytotoxic Effects in Sertoli Cells Resulting in Reproductive Dysfunction in Male Mice

miR‐142‐5p protects against osteoarthritis through competing with lncRNA XIST

Longitudinal expression profiling of CD4+ and CD8+ cells in patients with active to quiescent giant cell arteritis

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