Up-regulation of Nrf2-antioxidant signaling by Açaí (Euterpe oleracea Mart.) extract prevents oxidative stress in human endothelial cells

Soares, Elaine R.; Monteiro, Elisa Bernardes; de, Graziele Freitas; Inada, Kim Ohanna Pimenta; Torres, Alexandre Guedes; Perrone, Daniel; Soulage, Christophe O.; Monteiro, Mariana; Resende, Ângela Castro; Moura‐Nunes, Nathália; Costa, Cristiane; Daleprane, Julio Beltrame

doi:10.1016/j.jff.2017.07.035

Cited by 33 publications

(23 citation statements)

References 39 publications

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“…Patel, Chen, and Kavdia ()) reported that SOD2 expression is transient, according to our H 2 O 2 treatment in HUVECs, decreasing after 72 hr compared to other antioxidative enzymes. Regarding catalase, similar effects have been observed in another study with HUVECs, showing its level decreases after H 2 O 2 treatment and increase after a phenolic‐rich extract treatment (Soares et al, ).…”

Section: Discussionsupporting

confidence: 79%

“…When catalase activity was analyzed, although the H 2 O 2 treatment presented lower mRNA levels compared to control (Figure 5a), the activity levels of this enzyme were increased compared to control in the same condition (Figure 5e). Differences between mRNA and protein levels of catalase have been reported in another study regarding enzymes involved in oxidative stress metabolism (Soares et al, ). Taken together, these data indicate that these extracts have a protective effect against oxidative stress coming from lipids and inorganic sources (such as H 2 O 2 ), modulating intrinsic oxidative defenses in endothelial cells and restoring the oxidative balance.…”

Section: Discussionmentioning

confidence: 65%

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Protective effects of beet (Beta vulgaris) leaves extract against oxidative stress in endothelial cells in vitro

Silva

Morelli

Pavan

et al. 2020

Phytotherapy Research

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Beetroot is an herb used worldwide as a food product, raw material for food industry, ethanol production and source of food coloring. Beet leaves are an unconventional food with antioxidant properties, which might neutralize reactive oxygen species (ROS) induced by oxidized Low‐Density Lipoprotein (LDL) present in dyslipidemias. This study aimed to elucidate the effects of beet leaves on the suppression of LDL oxidative processes. Beet leaves extract was produced, characterized, and tested for its antioxidant capacity using endothelial cells in vitro. A model of human umbilical vein endothelial cells was used in various tests, including viability assay, molecular analysis of antioxidant genes, ROS labeling, and macrophage adhesion assay. The extract improved the antioxidative protection of endothelial cells against different agents including oxidized LDL‐cholesterol and H2O2. It acted on ROS directly due to its high content of natural antioxidants, but also due to the activation and improvement of cellular defenses such as Superoxide dismutase 1, Superoxide dismutase 2, and catalase. The inhibition of LDL‐mediated oxidative effects on endothelial cells may turn this unconventional food a functional food with great potential for phytotherapy of atherosclerosis as an adjuvant for medicinal treatments.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 65%

Protective effects of beet (Beta vulgaris) leaves extract against oxidative stress in endothelial cells in vitro

Silva

Morelli

Pavan

et al. 2020

Phytotherapy Research

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“…The non‐cytotoxic concentration range of ASE in HUVECs was previously described (Soares et al. ), indicating the preservation of cellular metabolic activity at 10 µg ml −1 of ASE.…”

Section: Methodsmentioning

confidence: 55%

“…In all experiments the concentrations of IS and pCS were 63 µg ml −1 (250 µM) and 40 µg ml −1 (212 µM), respectively, corresponding to the concentrations found in humans in end-stage renal disease (Duranton et al 2012 (Cohen et al 2007). The non-cytotoxic concentration range of ASE in HUVECs was previously described (Soares et al 2017), indicating the preservation of cellular metabolic activity at 10 µg ml −1 of ASE.…”

Section: Açaí Seed Extractmentioning

confidence: 61%

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Uraemic toxin‐induced inflammation and oxidative stress in human endothelial cells: protective effect of polyphenol‐rich extract from açaí

Monteiro

Soares

Trindade

et al. 2020

Experimental Physiology

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New Findings What is the central question of this study?Does a polyphenol‐rich extract from açaí have a potential role in preventing uraemic toxin‐induced endothelial cell dysfunction? What is the main finding and its importance?Polyphenols from açaí prevented cell death, restored migratory capacity, protected from inflammation and contributed to the restoration of the antioxidant response in endothelial cells exposed to uraemic toxins. The protective role of açaí against toxic effects exerted by uraemic toxins presents a potential new therapeutic target in endothelial cells. Abstract In chronic kidney disease (CKD), progressive loss of kidney function results in the accumulation of protein‐bound uraemic toxins such as p‐cresyl sulfate (pCS) and indoxyl sulfate (IS). Among strategies to ameliorate the harmful actions of uraemic toxins, phenolic compounds have been extensively studied. The main goal of this work was to evaluate the antioxidant and anti‐inflammatory actions of phenolic‐rich açaí seed extract (ASE) in response to endothelial dysfunction induced by IS and pCS, in human umbilical vein endothelial cells (HUVECs). Cells were treated with ASE (10 µg ml−1) in the presence or absence of IS (61 µg ml−1) and pCS (40 µg ml−1). Cell viability, cell death, cell migratory capacity and inflammatory biomarker expression were evaluated. Cellular antioxidant response was measured through the activity and expression of antioxidant enzymes, and oxidative damage was evaluated. IS and pCS lowered cell viability, triggered cell death and lowered the migratory capacity in endothelial cells (P < 0.05). ASE prevented cell death and restored the migratory capacity in cells exposed to IS. Both toxins up‐regulated pro‐inflammatory cytokine expression, and ASE was able to beneficially counteract this effect. Tumour necrosis factor‐α secretion was greater in uraemic toxin‐treated cells and ASE reversed this phenomenon in cells treated with both toxins concomitantly (P < 0.05). With regard to the antioxidant response, superoxide dismutase expression was strikingly lower in cells treated with both toxins, and ASE inhibited this harmful effect (P < 0.05). From the results, we conclude that ASE exerted protective effects on inflammation and oxidative stress caused by uraemic toxins (particularly by IS) in human endothelial cells.

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Brazilian (North and Northeast) Fruit By‐Products

Silva

Sousa

Sabino

et al. 2019

Food Wastes and By‐products

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Up-regulation of Nrf2-antioxidant signaling by Açaí (Euterpe oleracea Mart.) extract prevents oxidative stress in human endothelial cells

Cited by 33 publications

References 39 publications

Protective effects of beet (Beta vulgaris) leaves extract against oxidative stress in endothelial cells in vitro

Protective effects of beet (Beta vulgaris) leaves extract against oxidative stress in endothelial cells in vitro

Uraemic toxin‐induced inflammation and oxidative stress in human endothelial cells: protective effect of polyphenol‐rich extract from açaí

Brazilian (North and Northeast) Fruit By‐Products

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