Up-regulation of multiple serine proteinases during earthworm tail regeneration

Cho, Sung Jin; Cho, Pyo Yun; Lee, Myung Sik; Na, Youngeun; Lee, Joo Hun; Koh, Ki Seok; Choo, Jong Kil; Park, Soon Cheol

doi:10.1080/07924259.2001.9652710

Cited by 10 publications

(8 citation statements)

References 28 publications

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“…The availability and functional annotation of the EST resource, and the subsequent clustering to identify a set of gene objects has allowed the first high density microarray for L. rubellus to be fabricated. The development of this 8,000-feature array provides a valuable resource to the earthworm community for future studies in areas such as tissue regeneration and immune system function, for which earthworms are already in use as model systems [ 58 , 59 ]. Through experimental studies, we have clearly demonstrated the capacity of the microarray to provide novel insights by investigating the molecular basis of the responses of the species to environmental perturbations.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of developmental and xenobiotic responses in a keystone soil animal, the oligochaete annelid Lumbricus rubellus

et al. 2008

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BackgroundNatural contamination and anthropogenic pollution of soils are likely to be major determinants of functioning and survival of keystone invertebrate taxa. Soil animals will have both evolutionary adaptation and genetically programmed responses to these toxic chemicals, but mechanistic understanding of such is sparse. The clitellate annelid Lumbricus rubellus is a model organism for soil health testing, but genetic data have been lacking.ResultsWe generated a 17,000 sequence expressed sequence tag dataset, defining ~8,100 different putative genes, and built an 8,000-element transcriptome microarray for L. rubellus. Strikingly, less than half the putative genes (43%) were assigned annotations from the gene ontology (GO) system; this reflects the phylogenetic uniqueness of earthworms compared to the well-annotated model animals. The microarray was used to identify adult- and juvenile-specific transcript profiles in untreated animals and to determine dose-response transcription profiles following exposure to three xenobiotics from different chemical classes: inorganic (the metal cadmium), organic (the polycyclic aromatic hydrocarbon fluoranthene), and agrochemical (the herbicide atrazine). Analysis of these profiles revealed compound-specific fingerprints which identify the molecular responses of this annelid to each contaminant. The data and analyses are available in an integrated database, LumbriBASE.ConclusionL. rubellus has a complex response to contaminant exposure, but this can be efficiently analysed using molecular methods, revealing unique response profiles for different classes of effector. These profiles may assist in the development of novel monitoring or bioremediation protocols, as well as in understanding the ecosystem effects of exposure.

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Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of developmental and xenobiotic responses in a keystone soil animal, the oligochaete annelid Lumbricus rubellus

et al. 2008

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“…Upregulation of Distal-less expression occurred within 6 h of amputation, and it was maintained over the 24 h, which suggests involvement in the regenerative processes during early anterior regeneration. Communication between the nervous system and the surrounding tissues is essential for regeneration in the earthworm 2) and amphibia. 28) There is accumulating evidence that neurotransmitters and their corresponding ligand-gated ion channel receptors can regulate the growth, differentiation, and plasticity of developing central nerve system neurons in vertebrates.…”

Section: Expression Patterns Of Some Anterior Regenerationrelated Genesmentioning

confidence: 99%

“…1) It is thought that the earthworm is the highest evolutionary species capable of regenerating an anterior portion containing the central nerve system, heart, and clitellum. 2) Annelids constitute a large and diverse taxon of marine, freshwater, and terrestrial segmented worms. Traditionally regarded as comprising three distinct classes, polychaetes, oligochaetes, and leeches, 3) they are now thought to be paraphyletic with respect to Echiura, Pogonophora, and Sipuncula.…”

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Gene Expression Profile in the Anterior Regeneration of the Earthworm Using Expressed Sequence Tags

Cho

Lee

Tak

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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In order to gain insight into the gene expression profiles associated with anterior regeneration of the earthworm, Perionyx excavatus, we analyzed 1,159 expressed sequence tags (ESTs) derived from cDNA library early anterior regenerated tissue. Among the 1,159 ESTs analyzed, 622 (53.7%) ESTs showed significant similarity to known genes and represented 338 genes, of which 233 ESTs were singletons and 105 ESTs manifested as two or more ESTs. While 663 ESTs (57.2%) were sequenced only once, 308 ESTs (26.6%) appeared 2 to 5 times, and 188 ESTs (16.2%) were sequenced more than 5 times. A total of 803 genes were categorized into 15 groups according to their biological functions. Among 1,159 ESTs sequenced, we found several gene encoding signaling molecules, such as Notch and Distal-less. The ESTs used in this study should provide a resource for future research in earthworm regeneration.

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“…Because of the remarkable traits of the earthworm in evolutionary biology, such as flightless and legless locomotion, unfathomed diversity, evolutionary conservation, and ecotoxicology, the earthworm may be elevated from the status of a soil sentinel to that elusive entity, an ecologically relevant genetic model organism 9 . In addition, the earthworm is capable of regeneration and has significant benefits compared to planarian and hydras for exploring regenerative mechanisms, which include the following features 11,12 : (1) Complex phenotypic structures, such as an advanced central nervous system with memory function, a closed blood-vascular system, a coelom, and specialized body segmentation. Wound repair involves multiple tissues and is a complex regenerative process; (2) A relatively short regenerative cycle.…”

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confidence: 99%

Genome and single-cell RNA-sequencing of the earthworm Eisenia andrei identifies cellular mechanisms underlying regeneration

et al. 2020

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The earthworm is particularly fascinating to biologists because of its strong regenerative capacity. However, many aspects of its regeneration in nature remain elusive. Here we report chromosome-level genome, large-scale transcriptome and single-cell RNA-sequencing data during earthworm (Eisenia andrei) regeneration. We observe expansion of LINE2 transposable elements and gene families functionally related to regeneration (for example, EGFR, epidermal growth factor receptor) particularly for genes exhibiting differential expression during earthworm regeneration. Temporal gene expression trajectories identify transcriptional regulatory factors that are potentially crucial for initiating cell proliferation and differentiation during regeneration. Furthermore, early growth response genes related to regeneration are transcriptionally activated in both the earthworm and planarian. Meanwhile, single-cell RNAsequencing provides insight into the regenerative process at a cellular level and finds that the largest proportion of cells present during regeneration are stem cells.

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Up-regulation of multiple serine proteinases during earthworm tail regeneration

Cited by 10 publications

References 28 publications

Transcriptome profiling of developmental and xenobiotic responses in a keystone soil animal, the oligochaete annelid Lumbricus rubellus

Transcriptome profiling of developmental and xenobiotic responses in a keystone soil animal, the oligochaete annelid Lumbricus rubellus

Gene Expression Profile in the Anterior Regeneration of the Earthworm Using Expressed Sequence Tags

Genome and single-cell RNA-sequencing of the earthworm Eisenia andrei identifies cellular mechanisms underlying regeneration

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