Up-Regulation of miR-130b-3p Activates the PTEN/PI3K/AKT/NF-κB Pathway to Defense against Mycoplasma gallisepticum (HS Strain) Infection of Chicken

Yuan, Bo; Zou, Meiling; Zhao, Yabo; Zhang, Kang; Sun, Yingfei; Peng, Xiuli

doi:10.3390/ijms19082172

Cited by 34 publications

(24 citation statements)

References 63 publications

(78 reference statements)

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“…An increasing number of studies are being conducted on the crucial role played by microRNAs, such as gga-miR-1306-5p [32], gga-miR-10a [33], gga-miR-130b-3p [34], in mediating the innate host response of chickens against pathogens. The current study investigated the biological function of gga-miR-200a-3p in the immune response.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA gga-miR-200a-3p modulates immune response via MAPK signaling pathway in chicken afflicted with necrotic enteritis

Pham

Ban

Lee

et al. 2020

Vet Res

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MicroRNAs (miRNAs) are small non-coding RNAs that contribute to host immune response as post-transcriptional regulation. The current study investigated the biological role of the chicken (Gallus gallus) microRNA-200a-3p (gga-miR-200a-3p), using 2 necrotic enteritis (NE) afflicted genetically disparate chicken lines, 6.3 and 7.2, as well as the mechanisms underlying the fundamental signaling pathways in chicken. The expression of gga-miR-200a-3p in the intestinal mucosal layer of NE-induced chickens, was found to be upregulated during NE infection in the diseasesusceptible chicken line 7.2. To validate the target genes, we performed an overexpression analysis of gga-miR-200a-3p using chemically synthesized oligonucleotides identical to gga-miR-200a-3p, reporter gene analysis including luciferase reporter assay, and a dual fluorescence reporter assay in cultured HD11 chicken macrophage cell lines. Gga-miR-200a-3p was observed to be a direct transcriptional repressor of ZAK, MAP2K4, and TGFβ2 that are involved in mitogen-activated protein kinase (MAPK) pathway by targeting the 3′-UTR of their transcripts. Besides, gga-miR-200a-3p may indirectly affect the expression of protein kinases including p38 and ERK1/2 at both transcriptional and translational levels, suggesting that this miRNA may function as an important regulator of the MAPK signaling pathway. Proinflammatory cytokines consisting of IL-1β, IFN-γ, IL-12p40, IL-17A, and LITAF belonging to Th1 and Th17-type cytokines, were upregulated upon gga-miR-200a-3p overexpression. These findings have enhanced our knowledge of the immune function of gga-miR-200a-3p mediating the chicken immune response via regulation of the MAPK signaling pathway and indicate that this miRNA may serve as an important biomarker of diseases in domestic animals. © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article' s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article'

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Section: Discussionmentioning

confidence: 99%

MicroRNA gga-miR-200a-3p modulates immune response via MAPK signaling pathway in chicken afflicted with necrotic enteritis

Pham

Ban

Lee

et al. 2020

Vet Res

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“…Also, 14 of 45 miRNAs belonged to two or a maximum of four modules of group A, of which five miRNAs (miR-30b-5p, miR-34c-3p, miR-103a-3p, miR-362-3p, and miR-590-3p) belonged to three modules and miR-130b-3p belonged to four modules. miR-130b-3p has been reported to play particularly significant roles in cancer progression [38]. A study discovered that miR-30b-5p is a tumor suppressor in CRC through the USP22/Wnt/β-catenin signaling axis [39].…”

Section: Discussionmentioning

confidence: 99%

Contributions of Gene Modules Regulated by Essential Noncoding RNA in Colon Adenocarcinoma Progression

et al. 2020

BioMed Research International

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Noncoding RNAs (ncRNAs), especially microRNA (miRNA) and long noncoding RNA (lncRNA), have an impact on a variety of important biological processes during colon adenocarcinoma (COAD) progression. This includes chromatin organization, transcriptional and posttranscriptional regulation, and cell-cell signaling. The aim of this study is to identify the ncRNA-regulated modules that accompany the progression of COAD and to analyze their mechanisms, in order to screen the potential prognostic biomarkers for COAD. An integrative molecular analysis was carried out to identify the crosstalks of gene modules between different COAD stages, as well as the essential ncRNAs in the posttranscriptional regulation of these modules. 31 ncRNA regulatory modules were found to be significantly associated with overall survival in COAD patients. 17 out of the 31 modules (in which ncRNAs played essential roles) had improved the predictive ability for COAD patient survival compared to only the mRNAs of those modules, which were enriched in the core cancer hallmark pathways with closer interactions. These suggest that the ncRNAs’ regulatory modules not only exhibit close relation to COAD progression but also reflect the dynamic significant crosstalk of genes in the modules to the different malignant extent of COAD.

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“…PI3K signaling is important in regulating tumor cell proliferation, migration, and invasion [38,39]. Thus, PTEN is a negative regulator of cancer [40,41].…”

Section: Discussionmentioning

confidence: 99%

Tissue Mechanics and Expression of TROP2 in Oral Squamous Cell Carcinoma with Varying Differentiation

Zhang

Gao

et al. 2020

Preprint

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Background Trophoblast cell surface antigen 2 (TROP2) is overexpressed in many squamous cell carcinomas and contributes to tumor development and invasion. The correlation between the expression of TROP2 and occurrence and development of oral squamous cell carcinoma (OSCC) remains to be understood. MethodsWe have investigated the role of the specific marker TROP2 in OSCC patients using a combination of biophysics approaches. Analyzed 108 OSCC patient specimens with varying degrees of differentiation using hematoxylin and eosin staining and immunohistochemistry for TROP2 expression, along with Kaplan-Meier survival curve analysis and atomic force microscopy, to understand the morphology and mechanical properties of OSCC tissues. ResultsIn total, 34% poor differentiation underwent high TROP expression. TROP2 as risk factor about patient age (OR = 0.437, P = 0.039), tumor size (OR = 13.148, P = 0.000), TNM stage (OR = 0.141, P = 0.000) during low survival rate. Average surface roughness of low, medium, and highly differentiated OSCC tissues were 448.9 ± 54.8, 792.7 ± 83.6, and 993.0 ± 104.3 nm, respectively. The Pearson coefficient revealed a negative correlation between stiffness and TROP2 expression (r=-0.84, P < 0.01).Conclusion TROP2 expression increased with the decrease in survival rate and negatively correlated with the various degrees of differentiation and tissue mechanics. Taken together, our findings demonstrate that TROP2 may be an indicator of OSCC differentiation that leads to the altered mechanical properties (e.g., stiffness) of OSCC tissues.

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Up-Regulation of miR-130b-3p Activates the PTEN/PI3K/AKT/NF-κB Pathway to Defense against Mycoplasma gallisepticum (HS Strain) Infection of Chicken

Cited by 34 publications

References 63 publications

MicroRNA gga-miR-200a-3p modulates immune response via MAPK signaling pathway in chicken afflicted with necrotic enteritis

MicroRNA gga-miR-200a-3p modulates immune response via MAPK signaling pathway in chicken afflicted with necrotic enteritis

Contributions of Gene Modules Regulated by Essential Noncoding RNA in Colon Adenocarcinoma Progression

Tissue Mechanics and Expression of TROP2 in Oral Squamous Cell Carcinoma with Varying Differentiation

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