Up-regulation of microRNA targets correlates with symptom severity in Citrus sinensis plants infected with two different isolates of citrus psorosis virus

Marmisollé, Facundo E.; Arizmendi, Ailín; Ribone, Andres; Rivarola, Máximo Lisandro; García, María Laura; Reyes, Carina A.

doi:10.1007/s00425-019-03294-0

Cited by 7 publications

(8 citation statements)

References 69 publications

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“…MiRNAs play various roles in the growth and development of plants [ 33 , 34 ]. Many studies have reported that viral infection leads to changes in plant miRNA expression, and these changes are considered to be one of the reasons for the appearance of symptoms in infected plants [ 23 , 24 , 35 , 36 , 37 , 38 , 39 ]. In our previous work, we found that osa-miR171b is downregulated in RSV-infected rice plants.…”

Section: Discussionmentioning

confidence: 99%

A Novel miRNA in Rice Associated with the Low Seed Setting Rate Symptom of Rice Stripe Virus

Yuan¹,

Zhai

Zhou

et al. 2023

IJMS

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MicroRNAs play key regulatory roles in plant development. The changed pattern of miRNA expression is involved in the production of viral symptoms. Here, we showed that a small RNA, Seq119, a putative novel microRNA, is associated with the low seed setting rate, a viral symptom of rice stripe virus (RSV)-infected rice. The expression of Seq 119 was downregulated in RSV-infected rice. The overexpression of Seq119 in transgenic rice plants did not cause any obvious phenotypic changes in plant development. When the expression of Seq119 was suppressed in rice plants either by expressing a mimic target or by CRISPR/Cas editing, seed setting rates were extremely low, similar to the effects of RSV infection. The putative targets of Seq119 were then predicted. The overexpression of the target of Seq119 in rice caused a low seed setting rate, similar to that in Seq119-suppressed or edited rice plants. Consistently, the expression of the target was upregulated in Seq119-suppressed and edited rice plants. These results suggest that downregulated Seq119 is associated with the low seed setting rate symptom of the RSV in rice.

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Section: Discussionmentioning

confidence: 99%

A Novel miRNA in Rice Associated with the Low Seed Setting Rate Symptom of Rice Stripe Virus

Yuan¹,

Zhai

Zhou

et al. 2023

IJMS

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“…It is usually accepted that although the mechanisms through which viroids interact with their hosts are beginning to be dissected, the key triggering events and molecular mechanisms underlying viroid pathogenesis remain unclear [49,50], and CEVd and HSVd are no exception. As demonstrated by various types of citrus pathogens [51,52], further investigation of the molecular basis of viroid-host interactions is crucial to better understand the pathogenesis of viroids, and thus help to develop effective strategies to combat viroid diseases [50,53]. Important changes occur in the chloroplast, cell wall, peroxidase, and symporter activities upon infection of Etrog citron with CEVd [54].…”

Section: Signaling Pathways In Citrus Exocortis and Cachexia Pathogen...mentioning

confidence: 99%

A Current Overview of Two Viroids Prevailing in Citrus Orchards: Citrus Exocortis Viroid and Hop Stunt Viroid

Belabess¹,

Radouane²,

Sagouti³

et al. 2021

Citrus - Research, Development and Biotechnology

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Citrus exocortis viroid (CEVd) and hop stunt viroid (HSVd) are the main viroids circulating in all citrus-growing areas worldwide, and causing two well-known diseases on citrus trees; exocortis and cachexia, respectively. These viroids are small, covalently closed single-stranded RNA, allocated to the Pospiviroidae family. CEVd is the first viroid being described on citrus trees in 1948 in California. It is considered the largest citrus viroid at 371 nucleotides. It causes bark scaling disorder on the rootstock of citrus trees grafted on trifoliate orange and its hybrids and can cause dwarfing of trees grown on these rootstocks. HSVd was first observed in 1945 in Florida. It consists of 299 nucleotides. Stunting, chlorosis, bark gumming, stem pitting, decline, and depressions in the wood are the main symptoms of HSVd in mandarin and its hybrids. The introduction and propagation of infected budwoods are the main causes of viroids spread in citrus orchards. These agents are mechanically sap-transmissible and spread by contaminated tools. Neither seed transmission nor vectors have been reported for both viroids. Root transmission, though possible, would be overshadowed by mechanical transmission. Rapid and sensitive molecular-based detection methods specific to both viroids are available. Both diseases are controlled by using viroids-free budwoods for new plantations, launching budwood certification programs, and establishing a quarantine system for new citrus varieties introduction. The most important achievements in CEVd and HSVd researches are outlined in this chapter. This would help to provide a clearer understanding of the diseases they cause and contribute to the development of better control strategies.

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“…The latter was involved in the activation of programmed cell death and the reduction of chlorophyll biosynthesis. Therefore, the vast amount of both transcripts may contribute to the onset of necrosis and chlorosis symptoms on CPsV-infected plants [ 16 , 69 ]. Additionally, it has been proven that the CPsV 24K protein physically interacts with citrus miRNA precursors, thus affecting their processing and subsequent miRNA accumulation and target expression.…”

Section: Signaling Pathways In Citrus Psorosis Pathogenesismentioning

confidence: 99%

“…Six of them have been validated through the analysis of leaf degradome data. Their expression in infected samples was higher compared to healthy tissues and was positively correlated with symptom severity [ 69 ]. Therefore, the CPsV 24K protein is considered as a potential viral suppressor of RNA silencing (VSR) and effector of CPsV.…”

Section: Signaling Pathways In Citrus Psorosis Pathogenesismentioning

confidence: 99%

Citrus Psorosis Virus: Current Insights on a Still Poorly Understood Ophiovirus

et al. 2020

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Citrus psorosis was reported for the first time in Florida in 1896 and was confirmed as a graft-transmissible disease in 1934. Citrus psorosis virus (CPsV) is the presumed causal agent of this disease. It is considered as a type species of the genus Ophiovirus, within the family Aspiviridae. CPsV genome is a negative single-stranded RNA (-ssRNA) with three segments. It has a coat protein (CP) of 48 kDa and its particles are non-enveloped with naked filamentous nucleocapsids existing as either circular open structures or collapsed pseudo-linear forms. Numerous rapid and sensitive immuno-enzymatic and molecular-based detection methods specific to CPsV are available. CPsV occurrence in key citrus growing regions across the world has been spurred the establishment of the earliest eradication and virus-free budwood programs. Despite these efforts, CPsV remains a common and serious challenge in several countries and causes a range of symptoms depending on the isolate, the cultivar, and the environment. CPsV can be transmitted mechanically to some herbaceous hosts and back to citrus. Although CPsV was confirmed to be seedborne, the seed transmission is not efficient. CPsV natural spread has been increasing based on both CPsV surveys detection and specific CPsV symptoms monitoring. However, trials to ensure its transmission by a soil-inhabiting fungus and one aphid species have been unsuccessful. Psorosis disease control is achieved using CPsV-free buds for new plantations, launching budwood certification and indexing programs, and establishing a quarantine system for the introduction of new varieties. The use of natural resistance to control CPsV is very challenging. Transgenic resistance to at least some CPsV isolates is now possible in at least some sweet orange varieties and constitutes a promising biotechnological alternative to control CPsV. This paper provides an overview of the most remarkable achievements in CPsV research that could improve the understanding of the disease and lead the development of better control strategies.

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Up-regulation of microRNA targets correlates with symptom severity in Citrus sinensis plants infected with two different isolates of citrus psorosis virus

Cited by 7 publications

References 69 publications

A Novel miRNA in Rice Associated with the Low Seed Setting Rate Symptom of Rice Stripe Virus

A Novel miRNA in Rice Associated with the Low Seed Setting Rate Symptom of Rice Stripe Virus

A Current Overview of Two Viroids Prevailing in Citrus Orchards: Citrus Exocortis Viroid and Hop Stunt Viroid

Citrus Psorosis Virus: Current Insights on a Still Poorly Understood Ophiovirus

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