Up-regulation of long non-coding RNA SPRY4-IT1 promotes tumor cell migration and invasion in lung adenocarcinoma

Abstract

Long non-coding RNAs (lncRNAs) play important role in a variety of biological processes, and are tightly associated with tumorigenesis and cancer prognosis. However, the potential power of lncRNA signatures in predicting survival of patients with lung adenocarcinoma (LUAD) has not been investigated. Here, we identified a four-lncRNA signature (SPRY4-IT1, LINC00941,GPR158-AS1 and KCNK15-AS1) displaying prognostic values for LUAD using The Cancer Genome Atlas (TCGA) dataset. Based on the four-lncRNA signature, the LUAD patients can be classified into high-risk and low-risk groups with significantly different survival. We further validated the expression of SPRY4-IT1 in LUAD tissues and corresponding normal lung tissues using quantitative real-time PCR in Chinese LUAD patients. The results showed that SPRY4-IT1 was significantly up-regulated in LUAD tissues. Further analysis indicated that LUAD patients with high SPRY4-IT1 expression have significantly poorer overall survival in Chinese LUAD patients. Moreover, knock-down of SPRY4-IT1 can inhibit the lung cancer cell migration and invasion. The present work indicated that SPRY4-IT1 may play a pivotal role in promoting tumor migration and invasion in LUAD. Our work implicates the promising effect of SPRY4-IT1 on the prognosis of LUAD.

Long non‐coding RNAs in melanoma

“…It was initially identified to be upregulated in melanoma . In recent years, emerging studies have demonstrated the deregulation and pathogenic roles of SPRY4‐IT1 in human cancers, including lung cancer, gastric cancer, breast cancer and colorectal cancer . In melanoma, SPRY4‐IT1 expression was predominantly localized in the cytoplasm .…”

“…43 In recent years, emerging studies have demonstrated the deregulation and pathogenic roles of SPRY4-IT1 in human cancers, including lung cancer, gastric cancer, breast cancer and colorectal cancer. [44][45][46][47] In melanoma, SPRY4-IT1 expression was predominantly localized in the cytoplasm. 48 Knockdown of SPRY4-IT1 impeded cell proliferation and differentiation but promoted apoptosis in melanoma cells.…”

Diagnostic and Prognostic Potential of Circulating Long Non-Coding RNAs in Non Small Cell Lung Cancer

“…Zhang et al found SPRY4-IT1 was significantly up-regulated in LUAD tissues (not in LSCC) and further analysis show that the LUAD patients with high SPRY4-IT1 expression exhibit significantly poorer overall survival than those patients with low SPRY4-IT1 expression [33].…”

The long non‐coding RNA SPRY4‐IT1: An emerging player in tumorigenesis and osteosarcoma

“…This lncRNA was originally reported to be highly upregulated in melanoma where it played an oncogenic role . Since its discovery, a growing number of studies have reported the aberrant expression of SPRY4‐IT1 in other cancer types, such as lung, breast, gastric, oesophageal and prostate cancers . In this review, we examine current evidence on the deregulation and function of SPRY4‐IT1 in human cancers (Table and Figure ).…”

“…17 Since its discovery, a growing number of studies have reported the aberrant expression of SPRY4-IT1 in other cancer types, such as lung, breast, gastric, oesophageal and prostate cancers. [19][20][21][22][23] In this review, we examine current evidence on the deregulation and function of SPRY4-IT1 in human cancers IT1 expression has been shown to predict survival of cancer patients. In this review, we summarize recent evidence concerning SPRY4-IT1 deregulation and the associated mechanisms in human cancers.…”