Up-regulation of LncRNA NEAT1 induces apoptosis of human placental trophoblasts

Fan, XuFei; Zheng, Xiaodong; Jianyi, Lou; Ye, Liyan; Yan, Tao; Hu, Min

doi:10.1080/10715762.2020.1826468

Cited by 10 publications

(5 citation statements)

References 25 publications

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“…To date, few functional lncRNAs have been described in trophoblast cells. For example, lncRNA NEAT1 and lncRNA-HZ04 were found to participant in apoptosis of human placental trophoblasts [ 15 ]. lncRNA-HZ04 promotes BPDE-induced human trophoblast cell apoptosis and miscarriage [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

“…Li, T et al revealed distinct mRNA and lncRNA expression profiles of decidual natural killer cells in early missed abortion [ 14 ]. Fan et al reported that LncRNA NEAT1 regulates apoptosis of human placental trophoblasts via miR-18a-5p axis [ 15 ]. However, there is little evidence regarding the global expression landscape of lncRNAs and mRNA in placental villus of URSA.…”

Section: Introductionmentioning

confidence: 99%

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LncRNA landscape and associated ceRNA network in placental villus of unexplained recurrent spontaneous abortion

Tang

Chen

et al. 2023

Reprod Biol Endocrinol

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Background Unexplained recurrent spontaneous abortion (URSA) is one of the most challenging conditions frustrates women of childbearing age profoundly. The gene expression patterns and biological characteristics of placental villus in patients with URSA remain largely unknown. The aim of our study was to identify potential lncRNAs as well as their action mechanisms in URSA. Method The ceRNA microarray was used to identify the mRNA and lncRNA expression profiles of URSA patients and normal pregnancy. Functional enrichment analyses for differentially expressed mRNAs in URSA were performed. Protein-protein interaction analysis of differentially expressed mRNAs was performed to identify hub genes and key modules. Subsequently, the co-dysregulated ceRNA network of URSA was established, and the enrichment analyses for the mRNAs in the ceRNA network was implemented. qRT-PCR was performed to validated the expression of key ENST00000429019 and mRNAs in URSA. Results We found that URSA placental villus have distinct mRNA and lncRNA expression profiles through ceRNA microarray, with a total of 347 mRNAs and 361 lncRNAs differentially expressed compared with controls. The functional enrichment analysis revealed that ncRNA processing, DNA replication, cell cycle, apoptosis, cytokine-mediated signaling pathway, ECM-receptor interaction were the potentially disrupted pathways in URSA patients. Then we constructed a co-dysregulated ceRNA network and found differentially expressed mRNAs were regulated by a small fraction of hub lncRNAs. Finally, we found a key network of ENST00000429019 and three cell proliferation or apoptosis related key mRNAs (CDCA3, KIFC1, NCAPH), and validated their expression and regulation in tissue and cellular levels. Conclusions This study identified a key ceRNA network, which might take part in URSA and correlate with cell proliferation and apoptosis. Optimistically, this study may deepen our apprehensions about the underlying molecular and biological causes of URSA and provide an important theoretical basis for future therapeutic strategies for patients with URSA.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

LncRNA landscape and associated ceRNA network in placental villus of unexplained recurrent spontaneous abortion

Tang

Chen

et al. 2023

Reprod Biol Endocrinol

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“…functions as a competitive endogenous RNA of miR-651-3p to regulate YY1 on progress of spiral artery remodelling [190] uc.187 is up-regulated in preeclampsia and modulates proliferation, apoptosis, and invasion of HTR-8/SVneo cells [191] SPRY4-IT1 modulates trophoblast cell invasion and migration by affecting the epithelialmesenchymal transition [192] up-regulation modulates proliferation, migration, apoptosis, and network formation in HTR-8SV/neo cells [16] NN An lncRNA within intron 3 of the STOX2 gene which seems to regulate an essential trophoblast differentiation pathway [193] XIST has a role in X chromosome inactivation in females, a process that is paternal specific in the trophoblast and random in the fetus [194,195] lncRHOXF1 is the first example of an lncRNA from the X chromosome that regulates the host response to viral infections in human placental progenitor cells [196] LncRNA-TCL6 plays a role in early abortion by inhibiting placental implantation via the EGFR pathway [197] LncRNaIGF2-AS plays a role in recurrent miscarriage by regulating trophoblast functions [198] PVT1 is down-regulated in GDM and PE [199] HOTAIR plays an important role in suppressing angiogenesis of the human placenta by inhibiting the expression of VEGFA [171] NEAT1 is increased in intrauterine growth retardation (IUGR) placentas but the pathomechanism is not yet clear; up-regulation is inducing apoptosis in HTR-8/SVneo cells [200,201]…”

Section: Lncrna Function Citationmentioning

confidence: 99%

The Role of Non-Coding RNAs in the Human Placenta

Hufsky

Markert

Marz

2022

Cells

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Non-coding RNAs (ncRNAs) play a central and regulatory role in almost all cells, organs, and species, which has been broadly recognized since the human ENCODE project and several other genome projects. Nevertheless, a small fraction of ncRNAs have been identified, and in the placenta they have been investigated very marginally. To date, most examples of ncRNAs which have been identified to be specific for fetal tissues, including placenta, are members of the group of microRNAs (miRNAs). Due to their quantity, it can be expected that the fairly larger group of other ncRNAs exerts far stronger effects than miRNAs. The syncytiotrophoblast of fetal origin forms the interface between fetus and mother, and releases permanently extracellular vesicles (EVs) into the maternal circulation which contain fetal proteins and RNA, including ncRNA, for communication with neighboring and distant maternal cells. Disorders of ncRNA in placental tissue, especially in trophoblast cells, and in EVs seem to be involved in pregnancy disorders, potentially as a cause or consequence. This review summarizes the current knowledge on placental ncRNA, their transport in EVs, and their involvement and pregnancy pathologies, as well as their potential for novel diagnostic tools.

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“…4 Long noncoding RNAs (lncRNAs), a class of tissue-specific RNAs, are defined as transcribed RNA molecules without protein-coding functions. 6 According to the literature, multiple lncRNAs, such as HEIPP, 7 INHBA-AS1, 8 SNHG14, 9 FAM99A, 10 GASAL1, 11 NEAT1, 12 and H19 13 have been shown to be involved in the biological activity of trophoblast cells in PE. LncRNA apolipoprotein A-1 antisense RNA (APOA1-AS, NR_126,362) has gained much interest as an endogenous regulator of ApoA1 biogenesis.…”

Section: Introductionmentioning

confidence: 99%

The involvement of long noncoding RNA APOA1-AS in the pathogenesis of preeclampsia

Yang

Jiang

2022

Hum Exp Toxicol

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Objective Long noncoding RNAs (lncRNAs) are involved in preeclampsia (PE), and apolipoprotein A-1 antisense RNA (APOA1-AS) and has been found to be associated with a number of diseases. Our study aims to understand the involvement of APOA1-AS in PE. Methods Clinically, APOA1-AS expression in early-onset severe PE (EOSPE) patients and healthy controls was detected by real-time quantitative polymerase chain reaction. In vitro experiments were divided into control [coculturing trophoblasts with human uterine microvascular endothelial cells (UtMVECs)], TNF-α [coculturing trophoblasts with UtMVECs treated with tumor necrosis factor-α (TNF-α)], and TNF-α + control siRNA/APOA1-AS siRNA groups (UtMVECs transfected with control siRNA/APOA1-AS siRNA were cocultured with trophoblasts in the presence of TNF-α). The animals were divided into normal group, PE group (PE model was established by administrating nitro-L-arginine methyl ester (L-NAME) in rats), PE + control siRNA group (PE rats were treated with control siRNA), and PE + APOA1-AS siRNA group (PE rats were treated with APOA1-AS siRNA). Results Increased APOA1-AS was found in the placental tissues of EOSPE patients. APOA1-AS siRNA abolished the decreased integration of trophoblasts into UtMVEC networks induced by TNF-α. Furthermore, APOA1-AS siRNA improved pregnancy outcomes in PE rats with increased expression of vascular endothelial growth factor, placental growth factor, and fms-like tyrosine kinase receptor (Flt-1) but decreased expression of E-cadherin, intercellular cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). Conclusion Downregulation of APOA1-AS protected against TNF-α-induced inhibition of trophoblast integration into endothelial networks, thus exerting protective effects against PE rats.

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Up-regulation of LncRNA NEAT1 induces apoptosis of human placental trophoblasts

Cited by 10 publications

References 25 publications

LncRNA landscape and associated ceRNA network in placental villus of unexplained recurrent spontaneous abortion

LncRNA landscape and associated ceRNA network in placental villus of unexplained recurrent spontaneous abortion

The Role of Non-Coding RNAs in the Human Placenta

The involvement of long noncoding RNA APOA1-AS in the pathogenesis of preeclampsia

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