Up regulation of IL-6 is involved in di (2-ethylhexyl) phthalate (DEHP) induced migration and invasion of non small cell lung cancer (NSCLC) cells

Wang, Yingyi; Zhao, Ming; Liu, Jieying; Ni, Jianjiao; Jiao, Yuchen; Bai, Chunmei

doi:10.1016/j.biopha.2017.02.107

Cited by 27 publications

(16 citation statements)

References 26 publications

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“…On a separate note, invasion and migration of lung cancer cells were successfully demonstrated in A549 and H1299 lung cancer cells with DEHP exposure [116,207] The viability of A549 cells was significantly enhanced upon exposure to DEHP even as low as 5 µM [207]. A similar effect was observed in DEHP-treated H1299 cells.…”

Section: Biological Effects Of Phthalatesmentioning

confidence: 63%

“…A similar effect was observed in DEHP-treated H1299 cells. Although no significant effects were found in A549 and H1299 cells with lower DEHP exposures (<10 −4 M), nanomolar DEHP had stimulated in vitro migration and invasion of both lung cancer cells via upregulation of IL-6 mediated by the NF-κB signaling pathway [116].…”

Section: Biological Effects Of Phthalatesmentioning

confidence: 69%

“…In general, humans exposed to environmental concentrations of DEHP have been related to DNA damages [115]. In an in vitro study, induction of inflammatory response in the human lung epithelial cell line A549 by DEHP was confirmed by upregulation of proinflammatory cytokines interleukin-6 (IL-6) and interleukin-8 (IL-8) [116]. A similar effect was observed in A549 cells treated with mono-2-ethylhexyl phthalate (MEHP), which was a metabolite of DEHP [117].…”

Section: Background Information On Phthalates and Effects On Human Rementioning

confidence: 99%

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Multiple Stressor Effects of Radon and Phthalates in Children: Background Information and Future Research

Kwan

Nikezić

Roy

et al. 2020

IJERPH

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The present paper reviews available background information for studying multiple stressor effects of radon (222Rn) and phthalates in children and provides insights on future directions. In realistic situations, living organisms are collectively subjected to many environmental stressors, with the resultant effects being referred to as multiple stressor effects. Radon is a naturally occurring radioactive gas that can lead to lung cancers. On the other hand, phthalates are semi-volatile organic compounds widely applied as plasticizers to provide flexibility to plastic in consumer products. Links of phthalates to various health effects have been reported, including allergy and asthma. In the present review, the focus on indoor contaminants was due to their higher concentrations and to the higher indoor occupancy factor, while the focus on the pediatric population was due to their inherent sensitivity and their spending more time close to the floor. Two main future directions in studying multiple stressor effects of radon and phthalates in children were proposed. The first one was on computational modeling and micro-dosimetric studies, and the second one was on biological studies. In particular, dose-response relationship and effect-specific models for combined exposures to radon and phthalates would be necessary. The ideas and methodology behind such proposed research work are also applicable to studies on multiple stressor effects of collective exposures to other significant airborne contaminants, and to population groups other than children.

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Section: Biological Effects Of Phthalatesmentioning

confidence: 63%

Section: Biological Effects Of Phthalatesmentioning

confidence: 69%

Section: Background Information On Phthalates and Effects On Human Rementioning

confidence: 99%

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Multiple Stressor Effects of Radon and Phthalates in Children: Background Information and Future Research

Kwan

Nikezić

Roy

et al. 2020

IJERPH

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“…These results suggested the effects of IL-6, mediated by HBV, were probably responsible for the increased concentration of ANG. Additionally, extensive studies have revealed the role of IL-6 in the NF-κB pathway [45, 46]. Simultaneously, ANG can activate NF-κB pathway to inhibit apoptosis [35].…”

Section: Discussionmentioning

confidence: 99%

HBV Facilitated Hepatocellular Carcinoma Cells Proliferation by Up-Regulating Angiogenin Expression Through IL-6

Zhou

Yang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Patients with hepatitis B virus (HBV) infection are at a high risk of developing hepatocellular carcinoma (HCC). In this study, we aim to investigate the roles of HBV on angiogenin (ANG), as well as the effects on cell proliferation in presence of ANG down-regulation. Methods: Serum ANG was determined by ELISA. The expression of ANG mRNA and protein in HCC cell lines with or without HBV/HBx were determined. Western blot and ELISA were conducted to determine the effects of HBV/HBx on IL-6 expression. The role of IL-6 on ANG was evaluated by IL-6 recombinant protein or IL-6 neutralizing antibody. Immunofluorescence staining was used to detect the nuclear translocation of ANG. MTT was performed to evaluate the relative inhibition ratio. Result: In vivo experiments showed elevation of serum ANG in patients infected with HBV. In vitro experiments showed HBV and HBx contributed to the transcription and translation of ANG. ANG expression showed increase after IL-6 stimulation, and ANG protein decreased in the presence of IL-6 blocking with its antibody. HBV promoted nuclear translocation of ANG. Inhibiting ANG expression or blocking of nuclear transfer of ANG attenuated the 45S rRNA synthesis and cell proliferation. Conclusion: HBV and HBx protein can increase the level of ANG through IL-6. HBV and HBx contributed to the nuclear translocation of ANG. Cell proliferation was inhibited after inhibiting the expression or nuclear transfer of ANG.

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“…Recent data suggest that endocrine disruptors can promote NSCLC progression (Zhang et al, 2014;Rafael-Vázquez et al, 2018;Wang et al, 2017).These include natural and synthetic compounds, such as envi-ronmental estrogens, that interfere with the normal endocrine system of animals and humans and mimic the action of natural estrogen in the body (Markey et al, 2002). NSCLC cells express estrogen receptor (ER) and respond to estrogenic signals (Stabile et al, 2002), and estradiol stimulation of ERα enhances NSCLC cell migration and invasion (Siegfried et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Di(2-ethylhexyl) phthalate promotes lung cancer cell line A549 progression via Wnt/β-catenin signaling

Kim

2019

J. Toxicol. Sci.

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-Di(2-ethylhexyl) phthalate (DEHP) is widely used in polyvinylchloride-based materials and remains intact in the environment. Lungs are one route of entry of DEHP into the body; however, there is limited information on the effects and mechanism of action of DEHP on non-small cell lung cancer (NSCLC). Here, we addressed this by examining the effect of DEHP on the proliferation of A549 human lung adenocarcinoma cells by MTS assay. The induction of inflammation and epithelial-to-mesenchymal transition (EMT), as well as activation of the mitogen-activated protein kinase (MAPK) and Wnt/ β-catenin signaling pathways, were assessed by western blot and real-time polymerase chain reaction. Although there were discrepancies in the concentration, DEHP treatment enhanced A549 cell viability accompanied by increased mRNA and protein levels of inflammation-related factors, such as matrix metalloproteinase-9 and nuclear factor-κB. Additionally, EMT was activated in cells according to decreased E-cadherin and increased vimentin expression. Furthermore, MAPK pathway components, including phosphorylated p38 and c-Jun N-terminal kinase, and Wnt/β-catenin pathway components, including phosphorylated glycogen synthase kinase 3β and β-catenin, as well as their downstream genes c-Myc and cyclin D1, were upregulated in the presence of DEHP. These results suggest that DEHP promotes NSCLC progression by promoting cell proliferation, inflammation, and EMT via activation of Wnt/β-catenin signaling.

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Up regulation of IL-6 is involved in di (2-ethylhexyl) phthalate (DEHP) induced migration and invasion of non small cell lung cancer (NSCLC) cells

Cited by 27 publications

References 26 publications

Multiple Stressor Effects of Radon and Phthalates in Children: Background Information and Future Research

Multiple Stressor Effects of Radon and Phthalates in Children: Background Information and Future Research

HBV Facilitated Hepatocellular Carcinoma Cells Proliferation by Up-Regulating Angiogenin Expression Through IL-6

Di(2-ethylhexyl) phthalate promotes lung cancer cell line A549 progression via Wnt/β-catenin signaling

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