Up-regulation of extracellular copper/zinc superoxide dismutase mRNA after transmission of the filarial parasite Acanthocheilonema viteae in the vertebrate host Meriones unguiculatus

Lattemann, Claus T.; Matzen, Arne; Apfel, Heiko

doi:10.1016/s0020-7519(99)00073-9

Cited by 7 publications

(5 citation statements)

References 30 publications

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“…GFOLD value was calculated between freeliving adult female (F_AF) and parasitic adult female (P_AF) SOD3 possibly originated from SOD1, supported by the presence of extracellular SOD1-like in filaria Interestingly, we found seven SODs with signal peptide clustered with intracellular isoform in filaria ( Fig. 3a), five of which were confirmed with experimental evidence [37][38][39]. It should be admitted that typical SOD3 is extracellular, with signal peptide, and typical SOD1 is intracellular, without signal peptide.…”

Section: Overview Of Sod Gene Family Evolutionsupporting

confidence: 78%

Speciation and adaptive evolution reshape antioxidant enzymatic system diversity across the phylum Nematoda

Yang

et al. 2020

BMC Biol

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Background Nematodes have evolved to survive in diverse ecological niches and can be a serious burden on agricultural economy, veterinary medicine, and public health. Antioxidant enzymes in parasitic nematodes play a critical role in defending against host oxidative stress. However, the features of the evolution of antioxidant enzymes in the phylum Nematoda remain elusive. Results Here, we systematically investigated the evolution and gene expression of antioxidant enzymes in the genomes of 59 nematodes and transcriptomes of 20 nematodes. Catalase has been independently lost in several orders, suggesting that it is unnecessary for some nematodes. Unlike in mammals, phospholipid hydroperoxide glutathione peroxidase is widely distributed in nematodes, among which it has evolved independently. We found that superoxide dismutase (SOD) has been present throughout nematode evolutionary process, and the extracellular isoform (SOD3) is diverged from the corresponding enzyme in mammals and has undergone duplication and differentiation in several nematodes. Moreover, the evolution of intracellular and extracellular SOD isoforms in filaria strongly indicates that extracellular SOD3 originated from intracellular SOD1 and underwent rapid evolution to form the diversity of extracellular SOD3. We identify a novel putative metal-independent extracellular SOD presenting independently in Steinernema and Strongyloididae lineage that featured a high expression level in Strongyloides larvae. Sequence divergence of SOD3 between parasitic nematodes and their closest free-living nematode, the specifically high expression in the parasitic female stage, and presence in excretory-secretory proteome of Strongyloides suggest that SOD3 may be related with parasitism. Conclusions This study advances our understanding of the complex evolution of antioxidant enzymes across Nematoda and provides targets for controlling parasitic nematode diseases.

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Section: Overview Of Sod Gene Family Evolutionsupporting

confidence: 78%

Speciation and adaptive evolution reshape antioxidant enzymatic system diversity across the phylum Nematoda

Yang

et al. 2020

BMC Biol

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“…Nevertheless, as shown by the native gel experiment, extracellular SOD occurs in T. pseudospiralis. Such SOD was reported in O. volvulus and A. viteae (James et al 1994;Lattemann et al 1999). (GI21702730) Haemonchus contortus (GI1199519) Brugia pahangi (GI457482) Acanthocheilonema viteae (GI5441514) Onchocerca volvulus (GI134618) Saccharomyces cerevisiae (GI6322564) Aspergillus fumigatus (GI66851351) Schistoma mansoni (GI161121) Taenia solium (GI18252397) Homo sapiens (GI4507149) Mus musculus (GI134614) Drosophils melanogaster (GI8647) Fig.…”

Section: Discussionsupporting

confidence: 59%

“…Therefore, expression levels in infective-stage larvae and adult worms should be similar. However, Lattemann et al (1999) observed an upregulation of one of the SODs of A. viteae after the transmission of third-stage larvae into the mammalian host. The authors suggested that an increase in SOD activity may be necessary for the filarioid to counteract oxidative stress in the vertebrate host.…”

Section: Discussionmentioning

confidence: 90%

Cloning and characterization of the Cu/Zn superoxide dismutase of Trichinella pseudospiralis

Mak

2005

Parasitol Res

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Copper/zinc (Cu/Zn) superoxide dismutase (SOD) activity was identified for the first time in both crude somatic extracts (CE) and excretory/secretory (E/S) products of Trichinella pseudospiralis. It was the dominant SOD in infective-stage larvae. Native polyacrylamide gel electrophoresis of CE and E/S products yielded a prominent band, which was cyanide-sensitive and was partly inhibited by hydrogen peroxide in SOD assay. Cytosolic Cu/Zn SOD was cloned. The 471-bp full-length cDNA sequence contained an open reading frame of 157 amino acids. The gene contained three introns. Quantitative reverse transcription-polymerase chain reaction indicated that the expression of cytosolic Cu/Zn SOD was substantially higher in infective-stage larvae than in adult worms. Cluster analysis showed that the sequence of the Cu/Zn SOD of T. pseudospiralis, an adenophorean nematode, is related to those of Brugia pahangi, Acanthocheilonema viteae, Onchocerca volvulus, and Haemonchus contortus (all belonging to the sercenentean group).

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“…The immune sera were seen to specifically recognise AVSOD2 by Western blot analysis of total worm homogenate, thus confirming the results obtained in ELISA. Our results contrast the finding from natural infections of jirds with A. viteae , where only a small proportion of animals produce antibodies against recombinant AVSOD2 ( Lattemann, Matzen & Apfel in preparation). Immunization studies with purified recombinant EC‐SOD from O. volvulus that was applied with different adjuvants also failed to induce SOD‐specific immune response in jirds ( Lucius 1995).…”

Section: Introductioncontrasting

confidence: 99%

Immunogenicity of the extracellular copper/zinc superoxide dismutase of the filarial parasite Acanthocheilonema viteae delivered by a two‐phase vaccine strain of Salmonella typhimurium

Lattemann

Zhang

Matzen

et al. 1999

Parasite Immunology

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The recombinant extracellular copper/zinc superoxide dismutase of the filarial parasite Acanthocheilonema viteae (AVSOD2) was cloned in an expression vector under control of the bacteriophage T7 promoter and the resulting plasmid pLAT7 was introduced in tha aroA attenuated Salmonella typhimurium vaccine strain SL3261:pYZ84. This vaccine strain carries a chromosomally integrated two phase expression system containing inducible T7 RNA polymerase. The recombinant AVSOD2 was efficiently expressed, constituting up to 5% of the total bacterial protein. Furthermore, the plasmid vector containing the AVSOD2 cDNA was shown to be stable over a long period of time in the vaccine strain without antibiotic selection in vitro and in vivo. Jirds which were immunised orally with the recombinant vaccine strain expressing the A. viteae EC-SOD produced a strong humoral immune response.

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Up-regulation of extracellular copper/zinc superoxide dismutase mRNA after transmission of the filarial parasite Acanthocheilonema viteae in the vertebrate host Meriones unguiculatus

Cited by 7 publications

References 30 publications

Speciation and adaptive evolution reshape antioxidant enzymatic system diversity across the phylum Nematoda

Speciation and adaptive evolution reshape antioxidant enzymatic system diversity across the phylum Nematoda

Cloning and characterization of the Cu/Zn superoxide dismutase of Trichinella pseudospiralis

Immunogenicity of the extracellular copper/zinc superoxide dismutase of the filarial parasite Acanthocheilonema viteae delivered by a two‐phase vaccine strain of Salmonella typhimurium

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