Up-Regulation of Dicer, a Component of the MicroRNA Machinery, in Prostate Adenocarcinoma

Chiosea, Simion I.; Jelezcova, Elena; Chandran, Uma; Acquafondata, Marie; McHale, Teresa; Sobol, Robert W.; Dhir, Rajiv

doi:10.2353/ajpath.2006.060480

Cited by 318 publications

(306 citation statements)

References 52 publications

(58 reference statements)

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“…These changes in expression were further confirmed by both microarray analysis and by Q-PCR on selected miRNAs. It has been suggested that alterations in the expression levels of Dicer may explain global changes of miRNA expression in prostate adenocarcinoma (Chiosea et al, 2006;Ambs et al, 2008). However, in this study we did not observe significant differences in the expression levels of Dicer or Drosha between the studied patient groups (Supplementary Figure S2).…”

Section: Discussioncontrasting

confidence: 70%

Diagnostic and prognostic signatures from the small non-coding RNA transcriptome in prostate cancer

et al. 2011

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Prostate cancer (PCa) is the most frequent male malignancy and the second most common cause of cancer-related death in Western countries. Current clinical and pathological methods are limited in the prediction of postoperative outcome. It is becoming increasingly evident that small non-coding RNA (ncRNA) species are associated with the development and progression of this malignancy. To assess the diversity and abundance of small ncRNAs in PCa, we analyzed the composition of the entire small transcriptome by Illumina/ Solexa deep sequencing. We further analyzed the micro-RNA (miRNA) expression signatures of 102 fresh-frozen patient samples during PCa progression by miRNA microarrays. Both platforms were cross-validated by quantitative reverse transcriptase-PCR. Besides the altered expression of several miRNAs, our deep sequencing analyses revealed strong differential expression of small nucleolar RNAs (snoRNAs) and transfer RNAs (tRNAs). From microarray analysis, we derived a miRNA diagnostic classifier that accurately distinguishes normal from cancer samples. Furthermore, we were able to construct a PCa prognostic predictor that independently forecasts postoperative outcome. Importantly, the majority of miRNAs included in the predictor also exhibit high sequence counts and concordant differential expression in Illumina PCa samples, supported by quantitative reverse transcriptase-PCR. Our findings provide miRNA expression signatures that may serve as an accurate tool for the diagnosis and prognosis of PCa.

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Section: Discussioncontrasting

confidence: 70%

Diagnostic and prognostic signatures from the small non-coding RNA transcriptome in prostate cancer

et al. 2011

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“…A fraction of adenocarcinomas lose Dicer as a result of deletions at the Dicer locus. 76 The same group, 77 found by using a gene array analysis of 16 normal prostate tissue samples, 64 organ-confined, and 4 metastatic prostate adenocarcinomas, an up-regulation of major components of the miRNA machinery, including Dicer, in metastatic prostate adenocarcinoma. Immunohistochemical studies on a tissue microarray consisting of 232 prostate specimens confirmed up-regulation of Dicer in prostatic intraepithelial neoplasia and in 81% of prostate adenocarcinoma.…”

Section: Profiling Of Mirna Expression Using Real-time Rt Pcrmentioning

confidence: 99%

“…The presence of upregulated miRNA machinery may predict the susceptibility of prostate adenocarcinoma to RNA interferencebased therapy. 77 The miRNA precursor 26,60 and mature miRNA assays 27 were used to quantify the precursor and mature miRNA expression, respectively, in a number of cancer cell lines and tissues from patients with hepatocellular and pancreas cancer. 78 This comparison allowed us to determine the degree to which the miRNA precursors are being processed to mature miRNA.…”

Section: Profiling Of Mirna Expression Using Real-time Rt Pcrmentioning

confidence: 99%

MicroRNAs and cancer: Profile, profile, profile

Barbarotto

Schmittgen

Calin

2007

Intl Journal of Cancer

200

139

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MicroRNAs (miRNAs) represent a new class of small noncoding RNAs (ncRNAs, RNAs that do not codify for proteins) that can regulate gene expression by targeting messenger RNAs of protein coding genes and other ncRNA transcripts. miRNAs were recently found to be involved in the pathophysiology of all types of analyzed human cancers mainly by aberrant gene expression. This is characterized by abnormal levels of expression for mature and/or precursor miRNA transcripts in comparison to the corresponding normal tissues. miRNA profiling has allowed the identification of signatures associated with diagnosis, prognosis and response to treatment of human tumors. Therefore, miRNAs fingerprinting represents a new addition to the tools to be used by medical oncologists. ' 2007 Wiley-Liss, Inc.

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“…A reduced expression of Dicer in non-small cell lung cancer (30) or an overexpression of Dicer in prostate adenocarcinoma and in precursor lesions of lung adenocarcinoma (31,32) have been reported, indicating that an adequate level of Dicer expression may be required for maintaining normal cell functions.…”

Section: Dicermentioning

confidence: 99%

Protein Components of the microRNA Pathway and Human Diseases

Perron¹,

Provost²

2008

Methods in Molecular Biology

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SummaryMicroRNAs (miRNAs) are key regulators of messenger RNA (mRNA) translation known to be involved in a wide variety of cellular processes. In fact, their individual importance is reflected in the diseases that may arise upon the loss, mutation or dysfunction of specific miRNAs. It has been appreciated only recently that diseases may also develop when the protein components of the miRNA machinery itself are affected. The core enzymes of the major protein complexes involved in miRNA biogenesis and function, such as the ribonucleases III (RNases III) Drosha and Dicer as well as Argonaute 2 (Ago2), appear to be essential. However, the accessory proteins of the miRNA pathway, such as the DiGeorge syndrome critical region gene 8 (DGCR8) protein, Exportin-5 (Exp-5), TAR RNA binding protein (TRBP) and Fragile X mental retardation protein (FMRP), are each related, in various ways, to specific genetic diseases.

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Up-Regulation of Dicer, a Component of the MicroRNA Machinery, in Prostate Adenocarcinoma

Cited by 318 publications

References 52 publications

Diagnostic and prognostic signatures from the small non-coding RNA transcriptome in prostate cancer

Diagnostic and prognostic signatures from the small non-coding RNA transcriptome in prostate cancer

MicroRNAs and cancer: Profile, profile, profile

Protein Components of the microRNA Pathway and Human Diseases

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