Abstract:Background: To generate an in vitro system of the dermal epithelial-mesenchymal interaction we co-cultured HaCaT (keratinocytes) and HEPM (embryonic mesenchyme). This model allowed a local disjunction with preserved cell-cell communication. Methods: For the analysis of different protein expression patterns between co-and pure-cultured HaCaTs we performed 2D-electrophoresis and mass spectrometry. Afterwards the mass spectromertically identified protein 14-3-3σ was silenced by siRNA in HaCaT cells. Results: We a… Show more
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