Amboli region, consisting Amboli, Chaukul, Nene, and Parpoli villages is one of the biodiversity rich areas in northern Western Ghats. We opportunistically surveyed odonates from the region and prepared an annotated checklist of 93 species belonging to 12 families. We report 15 Western Ghats endemic species and six new records for the State of Maharashtra. We further present an updated checklist of Odonata of Maharashtra state with a total of 144 species.