UP-2.184: Different Concentrations of Triton X-100 To Decellularize Small Intestinal Submucosa

Villoldo, Gustavo; Loresi, Monica; Ielpi, Marcelo; Cambiasso, P.; Barbich, Mariana; Badiola, Francisco de; Ruiz, Eduardo; Giúdice, Carlos; Argibay, Pablo

doi:10.1016/j.urology.2009.07.403

Cited by 2 publications

(4 citation statements)

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“…Triton X‐100 is a non‐ionic detergent that disrupts the interactions between biological macromolecules more gently than SDS 26 . Decellularized SIS was prepared with different concentrations of Triton X‐100 (0.5%–2%) 27 . In addition, trypsin combined with EDTA can degrade C‐terminal polypeptides into arginine and lysine, which can change cell permeability and facilitate decellularization 28 .…”

Section: Resultsmentioning

confidence: 99%

“…26 Decellularized SIS was prepared with different concentrations of Triton X-100 (0.5%-2%). 27 In addition, trypsin combined with EDTA can degrade C-terminal polypeptides into arginine and lysine, which can change cell permeability and facilitate decellularization. 28 The SCK was decellularized with Triton X-100, SDS, and trypsin solutions, and the DNA contents of the skins after treatment were determined.…”

Section: Investigation Of Decellularization Methodsmentioning

confidence: 99%

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Study on the preparation and properties of acellular matrix from the skin of silver carp (Hypophthalmichthys molitrix)

Wei¹,

Zhang²,

Guo³

et al. 2023

J Biomed Mater Res

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Acellular matrices are mainly composed of mammalian tissues, and aquatic tissues with lower biological risks and less religious restrictions are considered alternatives to mammalian tissues. The acellular fish skin matrix (AFSM) has been commercially available. Silver carp has the advantages of farmability, high yield and low price, but there are few studies on the silver carp acellular fish skin matrix (SC-AFSM). In this study, an acellular matrix with low DNA and endotoxin was prepared from the skin of silver carp. After treatment with trypsin/sodium dodecyl sulfate and Triton X-100 solutions, the DNA content in SC-AFSM reached 11.03 ± 0.85 ng/mg, and the endotoxin removal rate was 96.8%. The porosity of SC-AFSM was 79.64% ± 0.17%, which is favorable for cell infiltration and proliferation. The relative cell proliferation rate of SC-AFSM extract was 117.79% ± 15.26%. The wound healing experiment showed that SC-AFSM had no adverse acute pro-inflammatory response, which had a similar effect as commercial products in promoting tissue repair. Therefore, SC-AFSM has great application potential in biomaterials.

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Section: Resultsmentioning

confidence: 99%

Section: Investigation Of Decellularization Methodsmentioning

confidence: 99%

Study on the preparation and properties of acellular matrix from the skin of silver carp (Hypophthalmichthys molitrix)

Wei¹,

Zhang²,

Guo³

et al. 2023

J Biomed Mater Res

View full text Add to dashboard Cite

show abstract

“…Triton X-100 is a non-ionic detergent that can disrupt interactions between biomacromolecules more gently than SDS. It is very commonly used alone or with other agents to generate matrices for skin repair derived from porcine SIS [82,99], porcine skin [68], porcine mesothelium [94], mouse skin [87], and rat flap [72]. Almost all protocols apply this detergent at the same concentration of 0.5% v/v, with a few using up to 1%.…”

Section: Detergentsmentioning

confidence: 99%

“…Almost all protocols apply this detergent at the same concentration of 0.5% v/v, with a few using up to 1%. The ability of Triton X-100 to fully decellularize a native tissue has been evaluated with porcine SIS exposure to increasing concentrations of detergent from 0.5% to 2% [99]. Acellular scaffolds were obtained after 48 h of immersion in all conditions, but ECM damage, especially loss of density, was observed for concentrations higher than 1%.…”

Section: Detergentsmentioning

confidence: 99%

Decellularized Scaffolds for Skin Repair and Regeneration

Dussoyer

Michopoulou²,

Rousselle

2020

Applied Sciences

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The skin is the largest organ in the body, fulfilling a variety of functions and acting as a barrier for internal organs against external insults. As for extensive or irreversible damage, skin autografts are often considered the gold standard, however inherent limitations highlight the need for alternative strategies. Engineering of human-compatible tissues is an interdisciplinary and active field of research, leading to the production of scaffolds and skin substitutes to guide repair and regeneration. However, faithful reproduction of extracellular matrix (ECM) architecture and bioactive content capable of cell-instructive and cell-responsive properties remains challenging. ECM is a heterogeneous, connective network composed of collagens, glycoproteins, proteoglycans, and small molecules. It is highly coordinated to provide the physical scaffolding, mechanical stability, and biochemical cues necessary for tissue morphogenesis and homeostasis. Decellularization processes have made it possible to isolate the ECM in its native and three-dimensional form from a cell-populated tissue for use in skin regeneration. In this review, we present recent knowledge about these decellularized biomaterials with the potential to be used as dermal or skin substitutes in clinical applications. We detail tissue sources and clinical indications with success rates and report the most effective decellularization methods compatible with clinical use.

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UP-2.184: Different Concentrations of Triton X-100 To Decellularize Small Intestinal Submucosa

Cited by 2 publications

References 0 publications

Study on the preparation and properties of acellular matrix from the skin of silver carp (Hypophthalmichthys molitrix)

Study on the preparation and properties of acellular matrix from the skin of silver carp (Hypophthalmichthys molitrix)

Decellularized Scaffolds for Skin Repair and Regeneration

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