Unveiling the potentials of bacteriocin (Pediocin L50) from Pediococcus acidilactici with antagonist spectrum in a Caenorhabditis elegans model

Chelliah, Ramachandran; Saravanakumar, Kandasamy; Daliri, Eric Banan‐Mwine; Kim, Joong‐Hark; Lee, Jung‐Kun; Jo, Hyeon-Yeong; Kim, Se‐Hun; Ramakrishnan, S.; Madar, Inamul Hasan; Wei, Shuai; Oh, Deog‐Hwan; Barathikannan, Kaliyan; Ofosu, Fred Kwame; Hwang, Su-Bin; Park, Eun-Ji; Yeong, Jung Da; Elahi, Fazle; Wang, Myeong‐Hyeon; Park, Jong Hwan; Ahn, Juhee; Kim, Dong Hwan; Park, Sung Jin; Oh, Deog‐Hwan

doi:10.1016/j.ijbiomac.2019.10.196

Cited by 12 publications

(15 citation statements)

References 95 publications

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“…These results showed many similarities with the previous reports that showed that different lactic acid bacterial strains possess different attaching and colonization efficiency in C. elegans gut [ 30 ]. The correlation between colonization and hydrophobicity was reported by chelliah et al [ 31 ], but some studies showed no correlation between the two functional properties [ 32 ]. However, in the current study, the probiotic yeast S. boulardii KT000032 showed efficient auto-aggregation compared with the other probiotic yeast strains.…”

Section: Resultsmentioning

confidence: 93%

“…The antimicrobial activity of CFS of isolated yeast strains using the disc diffusion method, S. boulardii (KT000032) showed higher antibacterial activity. Further, the minimum inhibitory concentration was determined based on the growth curve method [ 31 ]. Further, the minimum inhibitory concentration (MIC) of different plant extracts was determined based on growth inhibitory assay [ 33 , 34 ] against indicator pathogens such as E. coli , S. aureus , S. typi , S. dysenteriae, V. cholera and S. flexneri ( Figure 3 ).…”

Section: Resultsmentioning

confidence: 99%

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In Vitro Probiotic Evaluation of Saccharomyces boulardii with Antimicrobial Spectrum in a Caenorhabditis elegans Model

Chelliah

Kim

Daliri

et al. 2021

Foods

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In the present study, we screened for potential probiotic yeast that could survive under extreme frozen conditions. The antimicrobial and heat-stable properties of the isolated yeast strains Saccharomyces boulardii (S. boulardii) (KT000032, KT000033, KT000034, KT000035, KT000036, and KT000037) was analyzed and compared with commercial probiotic strains. The results revealed that the tested S. boulardii KT000032 strain showed higher resistance to gastric enzymes (bile salts, pepsin, and pancreatic enzyme) at low pH, with broad antibiotic resistance. In addition, the strain also showed efficient auto-aggregation and co-aggregation abilities and efficient hydrophobicity in the in-vitro and in-vivo C. elegens gut model. Further, the KT000032 strain showed higher antimicrobial efficiency against 13 different enteropathogens and exhibited commensal relationships with five commercial probiotic strains. Besides, the bioactive compounds produced in the cell-free supernatant of probiotic yeast showed thermo-tolerance (95 °C for two hours). Furthermore, the thermo-stable property of the strains will facilitate their incorporation into ready-to-eat food products under extreme food processing conditions.

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

In Vitro Probiotic Evaluation of Saccharomyces boulardii with Antimicrobial Spectrum in a Caenorhabditis elegans Model

Chelliah

Kim

Daliri

et al. 2021

Foods

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“…At present, P. acidilactici strains are widely used in the food industry, animal husbandry, and medical settings. The bacteriocins produced are known to inhibit the growth of pathogenic microorganisms in the host or to act as signalregulated peptides to regulate the health of the host (Chelliah et al, 2020). Although many studies have confirmed the promising effects of several probiotics in the treatment of constipation, the degree of alleviation provided by bacteriocinproducing P. acidilactici strains and their regulatory roles on the intestinal flora remain poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Pediococcus acidilactici Strains Improve Constipation Symptoms and Regulate Intestinal Flora in Mice

Qiao

Qiu

Tian

et al. 2021

Front. Cell. Infect. Microbiol.

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Constipation is a prevalent gastrointestinal disorder that seriously reduces the quality of life. Clinical studies have shown that a great change or severe imbalance occurs in the intestinal microbiota of people with constipation. This study explored whether bacteriocin-producing and non-bacteriocin-producing Pediococcus acidilactici strains resulted in differences in the alleviation of constipation and changes in the fecal flora in BALB/c mice. The constipation-related indicators, gastrointestinal regulatory peptides and gut microbiota were identified to evaluate their alleviating effects and underlying mechanisms. The time to the first black-stool defecation and the gastrointestinal transit rate in constipated mice were found to be somewhat improved by four P. acidilactici strains (P > 0.05). Moreover, there were significant differences in the level of most gastrointestinal regulatory peptides in the serum, as well as in the composition and abundance of intestinal microbiota in different groups (P < 0.05). At the phylum level, the relative abundance of Firmicutes was significantly increased, but those of Bacteroidetes and Proteobacteria were significantly reduced after the administration of four P. acidilactici strains for 14 d (P < 0.05). The levels of Bacteroides and genera from Enterobacteriaceae were significantly decreased, whereas Bifidobacterium and Lactobacillus were upregulated when bacteriocin-producing P. acidilactici CCFM18 and CCFM28 strains were provided in the diet (P < 0.05). The results indicated that although constipation-related symptoms were alleviated to only a limited degree, the administration of four P. acidilactici strains effectively regulated the gut flora and provided a potential health benefit to the host, especially the bacteriocin-producing P. acidilactici strains.

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“…Indeed, after preincubation with a molecule produced by P. acidilactici and molecular weight lower than 3 kDa (pediocin), nematodes showed increased survivals facing E. coli O157 and Helicobacter pylori infections. This increased survival was also associated with a significant decrease in the bacterial load in the gut of the worms (149).…”

Section: Lab Anti-pathogen Effectsmentioning

confidence: 88%

Caenorhabditis elegans, a Host to Investigate the Probiotic Properties of Beneficial Microorganisms

Poupet

Chassard

Nivoliez³

et al. 2020

Front. Nutr.

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Caenorhabditis elegans, a non-parasitic nematode emerges as a relevant and powerful candidate as an in vivo model for microorganisms-microorganisms and microorganisms-host interactions studies. Experiments have demonstrated the probiotic potential of bacteria since they can provide to the worm a longer lifespan, an increased resistance to pathogens and to oxidative or heat stresses. Probiotics are used to prevent or treat microbiota dysbiosis and associated pathologies but the molecular mechanisms underlying their capacities are still unknown. Beyond safety and healthy aspects of probiotics, C. elegans represents a powerful way to design large-scale studies to explore transkingdom interactions and to solve questioning about the molecular aspect of these interactions. Future challenges and opportunities would be to validate C. elegans as an in vivo tool for high-throughput screening of microorganisms for their potential probiotic use on human health and to enlarge the panels of microorganisms studied as well as the human diseases investigated.

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Unveiling the potentials of bacteriocin (Pediocin L50) from Pediococcus acidilactici with antagonist spectrum in a Caenorhabditis elegans model

Cited by 12 publications

References 95 publications

In Vitro Probiotic Evaluation of Saccharomyces boulardii with Antimicrobial Spectrum in a Caenorhabditis elegans Model

In Vitro Probiotic Evaluation of Saccharomyces boulardii with Antimicrobial Spectrum in a Caenorhabditis elegans Model

Pediococcus acidilactici Strains Improve Constipation Symptoms and Regulate Intestinal Flora in Mice

Caenorhabditis elegans, a Host to Investigate the Probiotic Properties of Beneficial Microorganisms

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