Unveiling the potential of perampanel and pregabalin in addressing pentylenetetrazole-induced electrographic alterations and neurobehavioral anomalies

Tariq, Maryam; Javaid, Sana; Ashraf, Waseem; Anjum, Syed Muhammad Muneeb; Rasool, Muhammad Fawad; Siddique, Farhan; Ahmad, Tanveer; Alsanea, Sary; Alasmari, Fawaz; Alqahtani, Faleh; Imran, Imran

doi:10.1016/j.biopha.2023.115935

Cited by 1 publication

(2 citation statements)

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“…The apparatus of Y-maze has three arms (A, B, and C), each comprising the dimensions of 40 × 8 × 15 cm. Animals to be tested were individually introduced in the middle of the apparatus, and their tendency to discover all arms was evaluated for 5 min . Due to the inherent curiosity of rodents toward novel surroundings, mice with remarkable cognitive abilities tend to investigate novel and previously undiscovered arms of the maze.…”

Section: Methodsmentioning

confidence: 99%

“…Animals to be tested were individually introduced in the middle of the apparatus, and their tendency to discover all arms was evaluated for 5 min. 31 Due to the inherent curiosity of rodents toward novel surroundings, mice with remarkable cognitive abilities tend to investigate novel and previously undiscovered arms of the maze. By recalling previously visited arms, the sequence of entries of animals in arms is altered, which is expressed as spontaneous alternation (SAP) by calculating through the following formula: % SAP = (number of alterations)/(total entries in three arms −2) × 100 2.6.5.…”

Section: Y-maze (Spontaneous Alteration)mentioning

confidence: 99%

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Neuroprotective Effect of Brivaracetam and Perampanel Combination on Electrographic Seizures and Behavior Anomalies in Pentylenetetrazole-Kindled Mice

Farooq,

Javaid,

Ashraf

et al. 2024

ACS Omega

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Pentylenetetrazole (PTZ)-induced kindling is a broadly used experimental model to study the anticonvulsive potential of new and existing chemical moieties with the aim of discovering drugs hindering seizure progression and associated neurological comorbidities. In the present study, the impact of brivaracetam (BRV) (10 and 20 mg/kg) as monotherapy as well as in combination with 0.25 mg/kg of perampanel (PRP) was investigated on seizure progression with simultaneous electroencephalographic changes in PTZ kindling mouse model. Subsequently, mice were experimentally analyzed for anxiety, cognition, and depression after which their brains were biochemically evaluated for oxidative stress. The outcomes demonstrated that BRV alone delayed the kindling process, but BRV + PRP combination significantly (p < 0.0001) protected the mice from seizures of higher severity and demonstrated an antikindling effect. The PTZ-kindled mice exhibited anxiety, memory impairment, and depression in behavioral tests, which were remarkably less (p < 0.001) in animals treated with drug combination (in a dose-dependent manner) as these mice explored central, illuminated, and exposed zones of open-field test, light/dark box, and elevated plus maze. Moreover, memory impairment was demonstrated by kindled mice, which was significantly (p < 0.001) protected by BRV + PRP as animal's spontaneous alteration, object discrimination, and step-through latencies were increased in various tests employed for the assessment of cognitive abilities. The brains of PTZkindled mice had increased malondialdehyde and reduced antioxidant enzymes while treatment with BRV + PRP combination prevented kindling-induced elevation in oxidative markers. The outcomes of this study demonstrate that combining the PRP at low dose augmented the antiseizure properties of BRV as both drugs when administered simultaneously hindered the process of kindling by reducing PTZ-induced excessive electrical activity and oxidative stress in the brain.

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Section: Methodsmentioning

confidence: 99%

Section: Y-maze (Spontaneous Alteration)mentioning

confidence: 99%