Unveiling shared genetic regulators for plant architectural and biomass yield traits in sorghum

Abstract

Sorghum is emerging as an ideal genetic model for designing high-biomass bioenergy crops. Biomass yield, a complex trait influenced by various plant architectural features, is typically regulated by numerous genes. This study aims to dissect the genetic mechanisms underlying fourteen plant architectural and ten biomass yield traits in a sorghum association panel (SAP) across two growing seasons. We identified 321 associated loci via genome-wide association studies involving 234,264 single nucleotide polymorphisms (SNPs). These loci encompass both genes with a priori links to biomass traits, such as maturity, dwarfing (Dw), leafbladeless1, cryptochrome, and several loci not previously linked to roles in determining these traits. We identified 22 pleiotropic loci associated with variation in multiple phenotypes. Three of these loci, located on chromosomes 3 (S03_15463061), 6 (S06_42790178; Dw2), and 9 (S09_57005346; Dw1), exert significant and consistent effects on multiple traits. Additionally, we identified three genomic hotspots on chromosomes 6, 7, and 9, containing multiple SNPs associated with variation in plant architecture and biomass yield traits. Positive correlations were observed among linked SNPs close to or within the same genomic regions. Thirteen haplotypes were identified from these positively correlated SNPs on chr 6, with haplotypes 8 and 11 emerging as optimal combinations, exhibiting pronounced effects on the traits. Lastly, network analysis revealed that loci associated with flowering, plant heights, leaf characteristics, plant number, and tiller number per plant were highly interconnected with other genetic loci linked to plant architecture and biomass yield traits. The pyramiding of favorable alleles related to these traits holds promise for enhancing the future development of bioenergy sorghum crops.