Unveiling mosquito cryptic species and their reproductive isolation

Zheng, Xiao

doi:10.1111/imb.12666

Cited by 12 publications

(3 citation statements)

References 84 publications

(169 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The species is mainly found in coastal areas [ 60 ]. In disease investigation, a rapid and accurate identification of target species is essential, particularly to detect potential cryptic species which may be involved in disease transmission and ultimately affects the efficacy of control measures [ 61 , 62 ].…”

Section: Discussionmentioning

confidence: 99%

DNA Barcoding of Potential Mosquito Disease Vectors (Diptera, Culicidae) in Jazan Region, Saudi Arabia

Noureldin¹,

Tan

Daffalla³

et al. 2022

Pathogens

View full text Add to dashboard Cite

The conventional morphological characterization of mosquito species remains heavily used for species identification in Jazan, Saudi Arabia. It requires substantial expertise and time, as well as having difficulty in confirming identity of morphologically similar species. Therefore, to establish a reliable and accurate identification system that can be applied to understanding spatial distribution of local mosquito species from the Jazan region, DNA barcoding was explored as an integrated tool for mosquito species identification. In this study, 44 adult mosquito specimens were analyzed, which contain 16 species belong to three genera of potential mosquito disease vectors (Aedes, Anopheles, and Culex). The specimens were collected from the Jazan region located in southwest Saudi Arabia. These included old and preserved mosquito voucher specimens. In addition, we assessed the genetic distance based on the generated mitochondrial partial COI DNA barcodes to detect cryptic diversity across these taxa. Nine mosquito species belonging to three genera were successfully barcoded and submitted to GenBank, namely: Aedes aegypti, Aedes caspius, Aedes vexans, Aedes vittatus, Anopheles arabiensis, Culex pipiens, Culex quinquefasciatus, Culex sitiens, and Culex tritaeniorhynchus. Of these nine species, Aedes vexans, Aedes vittatus, Culex sitiens, and Culex tritaeniorhynchus were registered in GenBank for the first time from Saudi Arabia. The DNA barcodes generated a 100% match to known barcodes of these mosquito species, that also matched with the morphological identification. Ae. vexans was found to be either a case of cryptic species (subspecies) or a new species from the region. However, more research has to be conducted to prove the latter. This study directly contributes to the development of a molecular reference library of mosquito species from the Jazan region and Saudi Arabia. The library is essential for confirmation of species in support of existing mosquito surveillance and control programmes.

show abstract

Section: Discussionmentioning

confidence: 99%

DNA Barcoding of Potential Mosquito Disease Vectors (Diptera, Culicidae) in Jazan Region, Saudi Arabia

Noureldin¹,

Tan

Daffalla³

et al. 2022

Pathogens

View full text Add to dashboard Cite

show abstract

“…Anopheles mosquitoes are essential vectors in the distribution of the malarial parasite, Plasmodium , hence monitoring mosquito populations is a crucial factor in predicting malaria risk in a geographic region of interest. The number of mosquito species is estimated to be 3500, and they are divided into 41 genera (Zheng, 2020). Anopheles, Aedes, and Culex are three of these 41 genera that are thought to be crucial in the spread of human illness caused by mosquitoes (Adugna et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

18S rDNA as a tool for molecular characterization of mosquito species from various regions within Limpopo.

Hadebe,

Okpeku

2024

Preprint

View full text Add to dashboard Cite

Anopheles mosquitos are the primary vectors for the rapid spread of malaria in Limpopo, South Africa, negatively impacting all malaria elimination agenda; particularly for a country with vector control as one of the major malaria elimination strategies. The implementation of vector control strategies, however, depends on the accurate identification of mosquito vectors, which has been lacking in Limpopo as a result of earlier studies that mainly relied on morphological identification of vectors, which has numerous drawbacks that lead to misidentification of mosquitoes, thus resulting in the development and assignment of ineffective control strategies. All this together set back elimination strategies and programs. The present study therefore aimed to molecularly identify mosquitoes collected from various regions within Limpopo province. This was accomplished by examining genetic composition of mosquitos from different regions within Limpopo province, using molecular genetics techniques such as 18S rDNA-based PCR analysis, 18S rDNA sequencing, etc. This investigation was successful since 18S rDNA was able to identify every one of the 42 mosquito samples that were analysed, revealing that the samples belonged to 7 different Anopheles species and the majority of the species were molecularly demonstrated to have been misidentified morphologically. The outcomes of the molecular analysis, which involved comparing the query sequences to the sequences in GenBank, were supported by the close relationship among mosquitoes of the same species in maximum likelihood trees and the presence of high to 100% bootstrap support values, giving confidence to conclude that mosquitoes of the same species under study are genetically related. Due to a number of constraints covered in this study, morphological identification of mosquitoes is prone to misidentification of species, hence genetic characterization is recommended to be used as a confirmatory approach in the identification of the species.

show abstract

“…Infestation with maternally inherited endosymbiotic bacteria, in particular Wolbachia Hertig, 1936 (Rickettsiales: Anaplasmataceae), is an additional factor, causing a variety of effects on reproductive biology and evolution of their hosts (Stouthamer et al, 1999; Werren et al, 2008). Inducing the cytoplasmic incompatibility, Wolbachia strains may trigger the formation of isolated mitochondrial clades, similar to cryptic species (Brucker & Bordenstein, 2012; Bruzzese et al, 2022), or ensure reproductive isolation among the cryptic species (Dumas et al, 2016; Zheng, 2020), thus acting as an underestimated factor of speciation (Rokas, 2000; Werren, 1997). There is a general agreement that an assessment of endosymbiont infection is needed for understanding the cryptic speciation.…”

Section: Introductionmentioning

confidence: 99%

Ancistroceruswasps (Hymenoptera, Vespidae) from the Centre of Europe: Phylogeny, cryptic species, neutral and non‐neutral markers

Budrys,

Orlovskytė,

Lazauskaitė

et al. 2023

Zoologica Scripta

View full text Add to dashboard Cite

The aim of the study was to clarify the phylogenetic relationships among Northern European Ancistrocerus and comparison of the applicability of evolutionarily neutral and non‐neutral markers for reconstruction of phylogeny. We used a 19,400 bp long dataset that included parts of mitochondrial DNA, nuclear rDNA operon, and 10 nuclear protein‐coding genes. Application of molecular barcoding and species delimitation algorithms unveiled a presence of cryptic species, A. balticus sp. n., in the trap‐nesting wasp communities of the centre of Europe. We assessed the morphological, biological, and ecological differences of it from the sibling A. trifasciatus and updated the regional identification key. Phylogeny reconstruction using the neutral and the presumably non‐neutral markers resulted in different tree topologies. Evolutionary congruence of the rDNA operon with the other markers was relatively low. Evolutionary rate of the mitochondrial genes was 7–8 times as high as that of the exons of the nuclear genes, therefore, the mitochondrial markers overshadowed the nuclear ones in the phylogeny reconstructions. We assumed that at the speciation level, we might consider two different patterns of phylogeny: one based on evolutionary time and neutral changes, and the other based on adaptive evolutionary pathways under directional selection pressures. We assessed the effect of directional selection on the nuclear protein‐coding genes, applying the Spearman's rank correlation between pairwise phylogenetic distances among species, estimated using exons, and these distances, estimated using introns. One of these markers demonstrated a lack of positive correlation, implying a variable directional selection pressure on the coded protein. The publication has been registered on ZooBank: urn:lsid:zoobank.org:pub:13BD28D0‐736D‐4B2A‐B5CF‐4824BD4CDCFB.

show abstract

Unveiling mosquito cryptic species and their reproductive isolation

Cited by 12 publications

References 84 publications

DNA Barcoding of Potential Mosquito Disease Vectors (Diptera, Culicidae) in Jazan Region, Saudi Arabia

DNA Barcoding of Potential Mosquito Disease Vectors (Diptera, Culicidae) in Jazan Region, Saudi Arabia

18S rDNA as a tool for molecular characterization of mosquito species from various regions within Limpopo.

Ancistroceruswasps (Hymenoptera, Vespidae) from the Centre of Europe: Phylogeny, cryptic species, neutral and non‐neutral markers

Contact Info

Product

Resources

About