Unveiling Biosynthesis of the Phytohormone Abscisic Acid in Fungi: Unprecedented Mechanism of Core Scaffold Formation Catalyzed by an Unusual Sesquiterpene Synthase

Takino, Junya; Kozaki, Tamotsu; Sato, Yoshiro; Liu, Chengwei; Ozaki, Taro; Minami, Atsushi; Oikawa, Hideaki

doi:10.1021/jacs.8b08925

Cited by 55 publications

(54 citation statements)

References 27 publications

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“…It is synthesized in plants and some phytopathogenic fungi using two distinct pathways. Phytopathogenic fungi synthesize ABA through the mevalonate pathway with intermediates containing no more than 15 carbon atoms, which is also called the “direct pathway” (Hirai et al 2000; Izquierdo‐Bueno et al 2018; Takino et al 2018). Plants synthesize ABA using the carotenoid pathway, which is also known as the “indirect pathway.” This is initiated from the cleavage of a C 40 precursor known as β‐carotene (Nambara and Marion‐Poll 2005; Arc et al 2013).…”

Section: Metabolic Control Of Aba Levelsmentioning

confidence: 99%

Abscisic acid dynamics, signaling, and functions in plants

Chen

Bressan

et al. 2020

JIPB

923

628

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Abscisic acid (ABA) is an important phytohormone regulating plant growth, development, and stress responses. It has an essential role in multiple physiological processes of plants, such as stomatal closure, cuticular wax accumulation, leaf senescence, bud dormancy, seed germination, osmotic regulation, and growth inhibition among many others. Abscisic acid controls downstream responses to abiotic and biotic environmental changes through both transcriptional and posttranscriptional mechanisms. During the past 20 years, ABA biosynthesis and many of its signaling pathways have been well characterized. Here we review the dynamics of ABA metabolic pools and signaling that affects many of its physiological functions.

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Section: Metabolic Control Of Aba Levelsmentioning

confidence: 99%

Abscisic acid dynamics, signaling, and functions in plants

Chen

Bressan

et al. 2020

JIPB

923

628

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“…As reported previously, most terpene synthases are active in the presence of Mg 2+ ions [8,37]. To test the Mg 2+ dependency of Tvi09626, an in vitro assay was performed.…”

Section: Resultsmentioning

confidence: 99%

Genome mining in Trichoderma viride J1-030: discovery and identification of novel sesquiterpene synthase and its products

Sun¹,

Cai²,

Yuan³

et al. 2019

Beilstein J. Org. Chem.

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Sesquiterpene synthases in Trichoderma viride have been seldom studied, despite the efficiency of filamentous fungi for terpenoid production. Using the farnesyl diphosphate-overexpressing Saccharomyces cerevisiae platform to produce diverse terpenoids, we herein identified an unknown sesquiterpene synthase from T. viride by genome mining and determined the structure of its corresponding products. One new 5/6 bicyclic sesquiterpene and its esterified derivative were characterised by GC–MS and 1D and 2D NMR spectroscopy. To the best of our knowledge, this is the first well-identified sesquiterpene synthase from T. viride to date.

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“…37) A similar stepwise reconstitution strategy has been applied to synthesize fungal ribosomally generated peptides, 38) antifungal anhydrides, 39), 40) and fungal polyketides. Furthermore, we recently unveiled the long-standing mystery in the biosynthesis of plant hormone abscisic acid in fungi, 41) which is significantly different from that in plants. Recently, fungal gene expression has become popular, especially in pathway elucidation of fungal metabolites.…”

Section: Discovery Of Daases In Fungal Phytotoxin Biosynthesismentioning

confidence: 99%

Heterologous production of fungal natural products: Reconstitution of biosynthetic gene clusters in model host <i>Aspergillus oryzae</i>

Oikawa

2020

Proc. Jpn. Acad., Ser. B

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While exploring phytotoxic metabolites from phytopathogenic fungi in the 1970s, we became interested in biosynthetic enzymes that catalyze Diels–Alder reactions involving biosynthesis of several phytotoxins that we isolated. Target enzymes were successfully characterized, and this triggered the identification of various Diels–Alderases in a recent decade. Through our Diels–Alderase project in 1990s, we recognized a highly efficient expression system of various biosynthetic genes with Aspergillus oryzae as a host. With the development of tools such as genomic data and bioinformatics analysis to identify biosynthetic gene clusters for natural products, we developed a highly reliable methodology such as hot spot knock-in to elucidate the biosynthetic pathways of representative fungal metabolites including phytotoxic substances. This methodology allows total biosynthesis of natural products and genome mining using silent biosynthetic gene clusters to obtain novel bioactive metabolites. Further applications of this technology are discussed.

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Unveiling Biosynthesis of the Phytohormone Abscisic Acid in Fungi: Unprecedented Mechanism of Core Scaffold Formation Catalyzed by an Unusual Sesquiterpene Synthase

Cited by 55 publications

References 27 publications

Abscisic acid dynamics, signaling, and functions in plants

Abscisic acid dynamics, signaling, and functions in plants

Genome mining in Trichoderma viride J1-030: discovery and identification of novel sesquiterpene synthase and its products

Heterologous production of fungal natural products: Reconstitution of biosynthetic gene clusters in model host <i>Aspergillus oryzae</i>

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