“Unusual” Tissues and Sample Collection Strategies on Exhumed Bodies

Agostini, Vincenzo; Gino, Sarah; Inturri, S.; Marinò, Alberto; Staiti, N.; Sticchi, M.; Chiti, Enrica; Linarello, P.; Gentile, Guendalina; Primignani, Paola; Giriodi, M.; Bailo, Paolo; Piccinini, A.

doi:10.1016/j.fsigss.2019.09.066

Cited by 4 publications

(3 citation statements)

References 8 publications

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“…9 The opinion is then based only on examining hard tissues. 10 Increasing awareness of public about tort, the incidence of exhumation of deceased bodies are on rise. Exhumation should be done in day light.…”

Section: Retrospective Analysis Of 258 Consecutive Forensic Exhumatio...mentioning

confidence: 99%

Retrospective analysis of 258 consecutive forensic exhumations with emphasis on causative agents/cause of death.

Ejaz Awan,

Haya Afzal,

Ghulam Mustafa Yousfani

et al. 2023

j Muhammad Med Coll

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Abstract: Introduction: Exhumation is the removal of the corpse from its grave for determination of the cause of death. All the exhumation cases are authorized by the Judicial Magistrate are appropriate authority under section 176 CrPC (code of criminal procedure). There are other religious, social and cultural reasons the exhumation is carried out in different parts of world. Objective: To analyse and evaluate the findings from exhumed bodies and the factors which play role in the uncertainty in the determination of the cause of death. Methodology: This retrospective observational study performed for 258 consecutive forensic exhumations performed at Department of Forensic Medicine & Toxicology, PUMHSW, Nawabshah in district from 2017 to 2019. These corpses were evaluated retrospectively on the basis of the autopsy report and police papers. The demographic information, causative factors and relevant data retrieve and analyzed using SPSS ver.19. The purpose of the present study was to analyze and evaluate the findings, their causative agents observed on the exhumed bodies and the factors which play role in the uncertainty in the determination of the cause of death. Results: There were 200 males and 58 were females. The causative agents included were blunt/sharp force and firearms. 242 cases were from urban and 16 were from rural area. The maximum cases of exhumation were 228 having >42 years and minimum were 30 <50 years. Due to advanced stage of decomposition, the marks of violence on soft tissues were not observed, and the internal organs were also in a state of decomposition. Histopathology and toxicology reveal negative results. Conclusion: Delayed exhumation due to lengthy legal procedure, results in decomposition and disputed cause of death. The success rate in challenged cases depends mostly on the duration of exhumation, condition of the soil, regardless the cause, frequency of such claims is on rise. Keywords: Exhumation, grave, dead bodies, Autopsy, facial identification, false claims on cause of death.

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Section: Retrospective Analysis Of 258 Consecutive Forensic Exhumatio...mentioning

confidence: 99%

Retrospective analysis of 258 consecutive forensic exhumations with emphasis on causative agents/cause of death.

Ejaz Awan,

Haya Afzal,

Ghulam Mustafa Yousfani

et al. 2023

j Muhammad Med Coll

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“…However, this procedure is time-consuming, expensive, and requires multiple steps, which increases the risk of DNA contamination. Therefore, alternative sources of genetic material, such as eye tissue (cornea and sclera) or cartilage tissue, are being investigated [75]. Importantly, cartilage tissue is free from the chimerism phenomenon that follows bone marrow transplantation, so it seems to be an ideal material for postmortem genetic analysis of unidentified individuals [76].…”

Section: Forensic Genetic Analysismentioning

confidence: 99%

Cartilage Tissue in Forensic Science—State of the Art and Future Research Directions

Tomsia

Cieśla²,

Pilch-Kowalczyk³

et al. 2022

Processes

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Cartilage tissue performs many functions in the human body. The diseases and injuries affecting it are prevalent due to its slow regeneration rate. However, cartilage tissue is exceptionally important for its auspicious use in forensic medicine due to its slow postmortem degradation rate. The presented review summarizes the latest research on cartilage tissues and their current and potential applications in forensic science. It also describes the most important studies on using cartilage and its microscopic and macroscopic analyses to estimate the deceased age and determine postmortem interval (PMI) values and the crime weapon. Additionally, the review describes attempts to isolate DNA from cartilage tissue for individual identification. The review also mentions recent, less abundant studies on the cartilage in forensic toxicology and genetics. It points out further directions and prospects for research development on cartilage tissue and its promising use in forensic medicine

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“…However, processing may be timeconsuming and/or technically difficult and may depend on experience [15,16]. Cartilages and ligaments, like the Achilles tendon, are quite resistant to autolysis and putrefaction and thus well suited for molecular identification even in heavily decomposed bodies [17][18][19][20][21]. When preferred tissue is not available or is too degraded to generate complete genetic profiles, the analysis of intervertebral discs (IVDs) may be considered.…”

Section: Introductionmentioning

confidence: 99%

The human intervertebral disc as a source of DNA for molecular identification

Becker

Mahlke

Ritz‐Timme

et al. 2021

Forensic Sci Med Pathol

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Genetic analyses such as STR-typing are routinely used for identification purposes in forensic casework. Although genotyping techniques only require a minimum amount of DNA to provide a genetic profile, DNA quality differs not only between but also within tissues during ongoing decomposition. Initiated by a recent case where, due to the constitution of the body, preferred tissue was not available or only resulted in a partial and not usable DNA profile, the analysis of intervertebral discs as a source of DNA was considered. As the analysis of this tissue resulted in a high quality DNA profile a further study was performed in which thirty intervertebral discs dissected from bodies in different stages of decay were analyzed. All samples yielded good quality DNA in quantities suitable for STR-based amplification with no or only low degradation indices, resulting in complete genetic profiles. These results demonstrate the robustness of human intervertebral disc tissue as a source of DNA for molecular identification purposes.

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“Unusual” Tissues and Sample Collection Strategies on Exhumed Bodies

Cited by 4 publications

References 8 publications

Retrospective analysis of 258 consecutive forensic exhumations with emphasis on causative agents/cause of death.

Retrospective analysis of 258 consecutive forensic exhumations with emphasis on causative agents/cause of death.

Cartilage Tissue in Forensic Science—State of the Art and Future Research Directions

The human intervertebral disc as a source of DNA for molecular identification

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