Unusual substrates for enzymes which hydrolyse glycosidic bonds

Barnett, J. E. G.

doi:10.1016/0020-711x(75)90082-8

Cited by 2 publications

(1 citation statement)

References 25 publications

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“…Previously, in a series of experiments it was shown by Barnett et al that various glycosyl fluorides were hydrolyzed by certain glycosidases, and that hydrolysis could be reversed [38]. If a concentrated solution of c~-glucopyranosyl fluoride was incubated with immobilized a-glucosidase by transglycosylation isomaltose (6%), isomaltosyl fluoride (25%), and also panose, Glc a 1-6 Glc a 1-4Glc (6%) could be isolated (Fig.…”

Section: Glycohydrolases In Glycosylationmentioning

confidence: 99%

Applications of enzymes in synthetic carbohydrate chemistry

Thiem

1995

FEMS Microbiology Reviews

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By photosynthesis 200 billion tons of glucose are formed per annum most of which remains in the sugar state. Thus, the well-known properties of saccharides as the basis for structural materials and energy stores is well established. Increasingly, focus is being addressed to their functions as recognition molecule. Therefore, carbohydrate chemistry became en vogue again as documented by classical syntheses of complex hetero-oligosaccharides employing protecting and activating group chemistry. In recent years enzymatic methods for acylation and deacylation in saccharide chemistry came into use. Novel saccharides could be approached following aldolase-catalyzed de novo syntheses. The very complex task of stereospecific glycosylation was addressed by use of enzymes. With glycohydrolases via transglycosylation as well as with glycosyltransferases including cofactor regenerating circles advances were made. Modified acceptor and also donor substrates allowed access to oligosaccharides in preparative quantities. This paper will discuss these modem developments and will focus on some selected examples from others' and our own laboratory.

show abstract

Section: Glycohydrolases In Glycosylationmentioning

confidence: 99%

Applications of enzymes in synthetic carbohydrate chemistry

Thiem

1995

FEMS Microbiology Reviews

View full text Add to dashboard Cite

show abstract

Applications of enzymes in synthetic carbohydrate chemistry

Thiem

1995

FEMS Microbiology Reviews

View full text Add to dashboard Cite

By photosynthesis 200 billion tons of glucose are formed per annum most of which remains in the sugar state. Thus, the well-known properties of saccharides as the basis for structural materials and energy stores is well established. Increasingly, focus is being addressed to their functions as recognition molecule. Therefore, carbohydrate chemistry became en vogue again as documented by classical syntheses of complex hetero-oligosaccharides employing protecting and activating group chemistry. In recent years enzymatic methods for acylation and deacylation in saccharide chemistry came into use. Novel saccharides could be approached following aldolase-catalyzed de novo syntheses. The very complex task of stereospecific glycosylation was addressed by use of enzymes. With glycohydrolases via transglycosylation as well as with glycosyltransferases including cofactor regenerating circles advances were made. Modified acceptor and also donor substrates allowed access to oligosaccharides in preparative quantities. This paper will discuss these modern developments and will focus on some selected examples from others' and our own laboratory.

show abstract

Unusual substrates for enzymes which hydrolyse glycosidic bonds

Cited by 2 publications

References 25 publications

Applications of enzymes in synthetic carbohydrate chemistry

Applications of enzymes in synthetic carbohydrate chemistry

Applications of enzymes in synthetic carbohydrate chemistry

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