Unusual sequences in the murine immunoglobulin μ–δ heavy-chain region

Richards, Julia E.; Gillian, A. C.; Shen, Anna L.; Tucker, Philip W.; Blattner, F. R.

doi:10.1038/306483a0

Cited by 108 publications

(44 citation statements)

References 43 publications

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“…The autoradiographs depicted are from a parallel analysis of WEHI231 and MPCll x WEHI. A restriction map indicates the derivation of the probes for /H-C|X transcripts that avoid repetitive sequences [i and ii (Richards et al 1983)]. Restriction sites: BamHl (B); Bglll (Bg); Hindlll (H); £coRI (R); Sad (S); Xbal (X); Xhol (Xh).…”

Section: Post-transcriptional Events Are Almost Exclusively Responsibmentioning

confidence: 99%

The half-life of immunoglobulin mRNA increases during B-cell differentiation: a possible role for targeting to membrane-bound polysomes.

Mason¹,

Williams²,

Neuberger³

1988

Genes Dev.

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The increase in abundance of immunoglobulin heavy-chain (IgH) mRNA that accompanies development of a B cell into a plasma cell is mainly due to post-transcriptional events. By constructing |x genes, whose expression is under inducible control, we determined the half-life of pulsed JJL transcripts to be ~20 hr in plasmacytoma hosts and ~3 hr in B-cell lymphomas. Interestingly, a JJL gene with a mutated signal sequence that is found on free (rather than membrane-bound) polysomes decays in the plasmacytoma host with a shortened half-life of 3 hr. Thus, a change in the turnover rate of IgH mRNA plays an important role in the cell-type specificity of immunoglobulin gene expression; this change may be a consequence of the fact that IgH mRNA is located in the membrane-bound polysome fraction.

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Section: Post-transcriptional Events Are Almost Exclusively Responsibmentioning

confidence: 99%

The half-life of immunoglobulin mRNA increases during B-cell differentiation: a possible role for targeting to membrane-bound polysomes.

Mason¹,

Williams²,

Neuberger³

1988

Genes Dev.

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“…To examine sequences that might be involved in the differential processing mechanism, the 0.89-kilobase (kb) AccI-KpnI fragment in the middle of the 1.86-kb C4-M1 intron (20) was deleted (pp.AK: Fig. lb).…”

mentioning

confidence: 99%

Effects of intron length on differential processing of mouse mu heavy-chain mRNA.

Tsurushita¹,

Korn²

1987

Mol. Cell. Biol.

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Production of membrane-bound and secreted forms of mouse ,u heavy-chain mRNA is controlled by differential processing in a developmental-stage-specific manner. We have analyzed the effects of various deletions and insertions in the C4-M1 intron of the mouse ,u gene on the differential processing of ,u mRNA. We show that there is a correlation between the length of the C4-M1 intron and the molar ratio of membrane-bound to secreted ,u mRNAs, i.e., the shorter the C4-M1 intron, the higher the ratio. Since the poly(A) addition signal in the C4-M1 intron seems to be intact in the mutant ,u genes, it is likely that the efficiency of splicing of the C4-M1 intron is affected by changes in the intron length.

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“…Alternating polypurine or polypyrimidine tracts have been previously reported in other eukaryotic genomes such as (CT)25 in sea urchin histone spacer [20] and (CT)3o and (GA)28 in mouse IgC~, [21]. A (CT)9 tract sandwiched with poly(A) and poly(T) observed around a rat calmodulin pseudogene therefore is a new type of simple repetitive sequence.…”

Section: Resultsmentioning

confidence: 99%

A peculiar repetitive sequence in the rat genome

Nishimura

Sokabe

1986

FEBS Letters

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We report here a new type of peculiar repetitive sequence, A15T(TC)9T12, which was detected at 750 base pairs (bp) upstream of a rat calmodulin processed pseudogene by DNA sequencing of cloned DNA fragments. This sequence element could possibly form a cruciform structure with a 12‐AT‐pair stem, exposing (CT)9 sequences as a loop. S1 nuclease protection experiments failed to identify this element as a cruciform structure but instead detected an alternating purine pyrimidine tract at 50 bp downstream of this element. Total genomic Southern blotting showed that the rat genome contains only a few of these elements.

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Unusual sequences in the murine immunoglobulin μ–δ heavy-chain region

Cited by 108 publications

References 43 publications

The half-life of immunoglobulin mRNA increases during B-cell differentiation: a possible role for targeting to membrane-bound polysomes.

The half-life of immunoglobulin mRNA increases during B-cell differentiation: a possible role for targeting to membrane-bound polysomes.

Effects of intron length on differential processing of mouse mu heavy-chain mRNA.

A peculiar repetitive sequence in the rat genome

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