Unusual Polyamines from Baikalian Diatoms

Annenkov, Vadim V.; Verkhozina, O. N.; Zelinskiy, Stanislav N.; Shishlyannikova, Tatyana A.; Bridoux, Maxime C.; Danilovtseva, Elena N.

doi:10.1002/slct.201802032

Cited by 5 publications

(3 citation statements)

References 41 publications

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“…In the case of diatoms, LCPAs are present as post-translationally grafted side chains in specific proteins namely, silaffins [28]. These LCPAs contain up to two tens of partially methylated nitrogen atoms separated by trimethylene or tetramethylene fragments [29]. Some polymeric amines [30,31] capable of interacting with oligomers of silicic acid and forming stable composite nanoparticles were considered as models of cytoplasmic silicon containing vesicles.…”

Section: Introductionmentioning

confidence: 99%

Design of Oligonucleotide Carriers: Importance of Polyamine Chain Length

et al. 2018

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Amine containing polymers are extensively studied as special carriers for short-chain RNA (13–25 nucleotides), which are applied as gene silencing agents in gene therapy of various diseases including cancer. Elaboration of the oligonucleotide carriers requires knowledge about peculiarities of the oligonucleotide–polymeric amine interaction. The critical length of the interacting chains is an important parameter which allows us to design sophisticated constructions containing oligonucleotide binding segments, solubilizing, protective and aiming parts. We studied interactions of (TCAG)n, n = 1–6 DNA oligonucleotides with polyethylenimine and poly(N-(3-((3-(dimethylamino)propyl)(methyl)amino)propyl)-N-methylacrylamide). The critical length for oligonucleotides in interaction with polymeric amines is 8–12 units and complexation at these length can be accompanied by “all-or-nothing” effects. New dimethylacrylamide based polymers with grafted polyamine chains were obtained and studied in complexation with DNA and RNA oligonucleotides. The most effective interaction and transfection activity into A549 cancer cells and silencing efficiency against vascular endothelial growth factor (VEGF) was found for a sample with average number of nitrogens in polyamine chain equal to 27, i.e., for a sample in which all grafted chains are longer than the critical length for polymeric amine–oligonucleotide complexation.

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Section: Introductionmentioning

confidence: 99%

Design of Oligonucleotide Carriers: Importance of Polyamine Chain Length

et al. 2018

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“…According to previous studies, polyamines have been extracted from the frustule of Chaetoceros didymium , Coscinodiscus asteromphalus , Coscinodiscus granii , Cyclotella cryptica, Cylindrotheca fusiformis , Nitzschia angularis , and Thalassiosira pseudonana [44,45,46,47]. Even after thousands of years, polyamines could be still measured in the siliceous frustules buried in marine and lake sediments [64,65]. The polyamines found in the frustule are mainly LCPAs [66,67,68,69].…”

Section: Polyamine Composition Of Microalgaementioning

confidence: 99%

Polyamines in Microalgae: Something Borrowed, Something New

Lin

2018

Marine Drugs

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Microalgae of different evolutionary origins are typically found in rivers, lakes, and oceans, providing more than 45% of global primary production. They provide not only a food source for animals, but also affect microbial ecosystems through symbioses with microorganisms or secretion of some metabolites. Derived from amino acids, polyamines are present in almost all types of organisms, where they play important roles in maintaining physiological functions or against stress. Microalgae can produce a variety of distinct polyamines, and the polyamine content is important to meet the physiological needs of microalgae and may also affect other species in the environment. In addition, some polyamines produced by microalgae have medical or nanotechnological applications. Previous studies on several types of microalgae have indicated that the putative polyamine metabolic pathways may be as complicated as the genomes of these organisms, which contain genes originating from plants, animals, and even bacteria. There are also several novel polyamine synthetic routes in microalgae. Understanding the nature of polyamines in microalgae will not only improve our knowledge of microalgal physiology and ecological function, but also provide valuable information for biotechnological applications.

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“…In the case of diatoms, LCPAs are present as post-translationally grafted side chains in specific proteins namely, silaffins [20]. These LCPAs contain up to two tens of partially methylated nitrogen atoms separated by trimethylene or tetramethylene fragments [21]. Some polymeric amines [22,23] capable of interacting with oligomers of silicic acid and forming stable composite nanoparticles were considered as models of cytoplasmic silicon containing vesicles.…”

Section: Introductionmentioning

confidence: 99%

Design of the Oligonucleotide Carriers: Importance of Polyamine Chain Length

Annenkov¹,

Krishnan²,

Pal’shin³

et al. 2018

Preprint

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Amine containing polymers are extensively studied as special carriers for short-chain RNA (13–25 nucleotides) which are applied as gene silencing agent in gene therapy of various diseases including cancer. Elaboration of the oligonucleotide carriers requires knowledge about peculiarities of oligonucleotide - polymeric amine interaction. Critical length of the interacting chains is the important parameter which allows to design sophisticated constructions containing oligonucleotide binding segments, solubilizing, protective and aiming parts. We studied interaction of (TCAG)n, n=1-6 DNA oligonucleotides with polyethylenimine and poly(N-(3-((3-(dimethylamino)propyl)(methyl)amino)propyl)-N-methylacrylamide). Critical length for oligonucleotides in interaction with polymeric amines is 8-12 units and complexation at these length can be accompanied by "all-or-nothing" effects. New dimethylacrylamide based polymers with grafted polyamine chains were obtained and studied in complexation with DNA and RNA oligonucleotides. The most effective interaction and transfection activity into A549 cancer cells was found for a sample with average number of nitrogens in polyamine chain equal to 27, i.e. for a sample in which all grafted chains are longer the critical length for polymeric amine - oligonucleotide complexation.

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Unusual Polyamines from Baikalian Diatoms

Cited by 5 publications

References 41 publications

Design of Oligonucleotide Carriers: Importance of Polyamine Chain Length

Design of Oligonucleotide Carriers: Importance of Polyamine Chain Length

Polyamines in Microalgae: Something Borrowed, Something New

Design of the Oligonucleotide Carriers: Importance of Polyamine Chain Length

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