Unusual confinement properties of a water insoluble small peptide hydrogel

Singha, Nilotpal; Srivastava, Arpita; Pramanik, Bapan; Ahmed, Sahnawaz; Dowari, Payel; Chowdhuri, Sumit; Debnath, Ananya; Das, Debapratim

doi:10.1039/c9sc01754b

Cited by 39 publications

(57 citation statements)

References 50 publications

(60 reference statements)

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“…Incorporation of the hydrophobic tail at one end of the NDI moiety ensures that the peptide sequences remain face to face as NDI and Py units form CT complexes (Scheme 1A). Amongst all these pairs, PyKC 32 and NDI-1 (Scheme 1 and S3 †) were found to have the closest proximity of the -SH groups (4.07Å, Fig. 1, S1 and Table S2 †) in the energy minimized structures of the pairs as obtained from DFT calculations.…”

Section: Resultsmentioning

confidence: 94%

Freeze the dynamicity: charge transfer complexation assisted control over the reaction pathway

et al. 2019

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Section: Resultsmentioning

confidence: 94%

Freeze the dynamicity: charge transfer complexation assisted control over the reaction pathway

et al. 2019

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“…The developed hydrogelators could retain enormous quantity of water in their structure and form elastic gels which slowly biodegrades into smaller molecules due to proteolysis or hydrolysis. 26 The drug release study results revealed the initial burst release of MP which might be due to physical entrapments of MP, followed by sustain release profile. 27 The results could be attributed to the initial release of MP from the solution of 1P + MP which later self-assembled in presence of ALP entrapping the drug in hydrogel.…”

Section: Discussionmentioning

confidence: 95%

Alkaline-phosphatase triggered self-assemblies enhances the anti-inflammatory property of methylprednisolone in spinal cord injury

Zhang

Zhou

et al. 2020

Journal of Applied Biomaterials & Functional Materials

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Methylprednisolone sodium phosphate (MP) is an anti-inflammatory corticosteroid which is used in the treatment of spinal cord injury (SCI), however the overdose of MP has toxic effects Therefore it is prerequisite to develop novel approaches to overcome the side effects of MP and enhance its efficacy. In the present work, we have developed alkaline phosphatase (ALP) trigger self-assembly system of oligopeptides to physically entrap and locally deliver MP. The synthesis of Nap-Phe-Phe-Tyr(H2PO3)-OH (1P) was achieved using solid phase peptide synthesis and was characterized using mass spectroscopy. The 1P is a hydrogelator, which in presence of ALP self-assembles to form the hydrogel. During the self-assembly of 1P, MP was physically entrapped without losing the physical strength of hydrogel as revealed in the rheology study. The consistency of this hydrogel and the structure was characterized using circular dichroism. The MP was released from the hydrogel in a sustain manner and 80% of the drug release was observed at 120 h. The MP + 1P were non-toxic to the cells at lower concentration however toxicity increases with the increase in concentration of MP. Further, the in-vivo administration of MP + 1P significantly reduces the pro-inflammatory cytokines and the histological analysis revealed improvement in the SCI. In conclusion, it could be stated that the synthesis of 1P for the delivery of MP provides the novel opportunity in for the treatment of SCI.

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“…A series of characterization studies were performed to examine and compare the properties of the composite hydrogels to those of hydrogels prepared with the polymers alone or only with PyKC. PyKC forms disulfide linked dimers in neutral to basic conditions due to the presence of cysteine residue in the sequence [ 34 ]. These dimers have been previously shown to be crucial for the hydrogelation of PyKC [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

“…PyKC forms disulfide linked dimers in neutral to basic conditions due to the presence of cysteine residue in the sequence [ 34 ]. These dimers have been previously shown to be crucial for the hydrogelation of PyKC [ 34 ]. ESI-MS analyses of 24 h matured samples of HA/PyKC and Gel/PyKC composite hydrogels showed the presence of a peak corresponding to the PyKC dimer ( Figures S1 and S2 ).…”

Section: Resultsmentioning

confidence: 99%

“…In this context, we have recently described a tripeptide, PyKC ( Scheme 1 ), which forms a very tightly knitted network of thin fibers in the hydrogel state [ 34 , 35 ]. The glutathione responsive supramolecular PyKC hydrogel remains insoluble in water and other water miscible organic medium, as well as buffers of different pH (3–11), and human serum.…”

Section: Introductionmentioning

confidence: 99%

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The Effects of a Short Self-Assembling Peptide on the Physical and Biological Properties of Biopolymer Hydrogels

et al. 2021

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Hydrogel scaffolds have attracted much interest in the last few years for applications in the field of bone and cartilage tissue engineering. These scaffolds serve as a convenient three-dimensional structure on which cells can grow while sensing the native environment. Natural polymer-based hydrogels are an interesting choice for such purposes, but they lack the required mechanical properties. In contrast, composite hydrogels formed by biopolymers and short peptide hydrogelators possess mechanical characteristics suitable for osteogenesis. Here, we describe how combining the short peptide hydrogelator, Pyrene-Lysine-Cysteine (PyKC), with other biopolymers, can produce materials that are suitable for tissue engineering purposes. The presence of PyKC considerably enhances the strength and water content of the composite hydrogels, and confers thixotropic behavior. The hyaluronic acid-PyKC composite hydrogels were shown to be biocompatible, with the ability to support osteogenesis, since MC3 T3-E1 osteoblast progenitor cells grown on the materials displayed matrix calcification and osteogenic differentiation. The osteogenesis results and the injectability of these composite hydrogels hold promise for their future utilization in tissue engineering.

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Unusual confinement properties of a water insoluble small peptide hydrogel

Abstract: A water insoluble peptide-hydrogel that shows unique compartmentalization by not allowing any exchange to and from the hydrogel and can protect enzymes from denaturation.

Cited by 39 publications

References 50 publications

Freeze the dynamicity: charge transfer complexation assisted control over the reaction pathway

Freeze the dynamicity: charge transfer complexation assisted control over the reaction pathway

Alkaline-phosphatase triggered self-assemblies enhances the anti-inflammatory property of methylprednisolone in spinal cord injury

The Effects of a Short Self-Assembling Peptide on the Physical and Biological Properties of Biopolymer Hydrogels

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