Unusual Analyte Adsorption Effects on Inert Lc Components

Hambleton, Paul; Lough, W. J.; Maltas, John; Mills, Malcolm J.

doi:10.1080/10826079508010445

Cited by 12 publications

(7 citation statements)

References 3 publications

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“…Since higher water contents might even better mask residual silanol groups, both glass and bare silica were assumed to be inert in the set-up of our experiments. The use of columns, packed with small metal particles was also considered, although it is known that even stainless steel is not completely inert in all cases [27]. Since the packing of these columns was hampered by the high specific mass of the materials [22], in this respect only the use of a stainless steel pre-column filter was studied.…”

Section: Resultsmentioning

confidence: 99%

Characterization of low-molar-mass polymers by gradient polymer elution chromatography II. Solubility effects in the analysis of polyester resins under reversed-phase conditions

Philipsen¹,

Cooker

Claessens

et al. 1997

Journal of Chromatography A

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Section: Resultsmentioning

confidence: 99%

Characterization of low-molar-mass polymers by gradient polymer elution chromatography II. Solubility effects in the analysis of polyester resins under reversed-phase conditions

Philipsen¹,

Cooker

Claessens

et al. 1997

Journal of Chromatography A

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“…have been found by other workers, especially when injecting the analyte in a solvent which was of lower eluting power than the HPLC mobile phase. [6][7][8][9][10][11] There was no clear identification of the adsorption site(s) in most of these studies, although Macleod et al 8 suspected that adsorption was taking place on the injector rotor valve seal.…”

Section: Problems Of Adsorption Of Single Compounds In Hplc Injectorsmentioning

confidence: 99%

“…Otero et al 9 thought that the stainless-steel sample loop was responsible, rather than the injector itself, and Hambleton et al 10 discovered that non-steroidal anti-inflammatory drugs (NSAIDs) were being adsorbed onto a polymeric PEEK™ sample loop. However, Lough et al, 11 although also finding evidence of analyte (Indomethacin) adsorption onto the surface of the stainless-steel sample loop, demonstrated by a series of elegant experiments that the major site of adsorption was the injector Vespel™ rotor seal.…”

Section: Problems Of Adsorption Of Single Compounds In Hplc Injectorsmentioning

confidence: 99%

Differential adsorption of analyte and deuterated analogue onto the rotor seal of the injector in high‐performance liquid chromatography/electrospray ionisation mass spectrometry

Lord

2020

Rapid Comm Mass Spectrometry

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Rationale Quantitation of analytes by liquid chromatography/mass spectrometry (LC/MS) is now very widely used, usually with an appropriate internal standard, but this does not guarantee problem‐free analyses. Investigation of a problem that arose during method development is described, where it was surmised that differential adsorption onto the high‐performance liquid chromatography (HPLC) injector of the analyte (Rac GTPase inhibitor NSC23766) and its deuterated analogue, as internal standard, was taking place. Methods Samples were injected onto the HPLC system via either a Valco C14W.1 internal sample injector or a Rheodyne 7125 injector, both with dissimilar Vespel rotor seals, and linked to a quadrupole time‐of‐flight (QTOF) mass spectrometer. Alternatively, samples were infused directly into the mass spectrometer via a syringe‐driver. Electrospray ionisation in positive mode was used in both cases. Results Experiments demonstrated significant differential adsorption of the analyte (RAC inhibitor, NSC23766) and its tri‐deuterated internal standard onto the Vespel rotor seal, with the latter seeming to be preferentially adsorbed. The deuterated analogue also showed lower electrospray sensitivity, as demonstrated by syringe infusion of a mixture of the compounds, compared with their separate infusion at the same concentration. Conclusions This study has demonstrated the problem of differential adsorption onto HPLC Vespel rotor seals, and that the use of a stable‐isotope‐labelled analogue as internal standard does not guarantee the constancy of the analyte/internal response ratio in quantitative methods. The partial solution was to work at much higher concentration where adsorption, whilst still apparent, was relatively insignificant.

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“…165 However, even PEEK may exhibit problems with adsorption, as observed for instance during loop injection of nonsteroid antiinflammatory drugs. 175 In our experience, the material also suffers from fatigue after prolonged use with eluents containing high concentrations of acetonitrile, eventually leading to bursts. This may have prompted the recent introduction of gold-coated columns as a way of fighting protein adsorption.…”

Section: The Biocompatibility Issuementioning

confidence: 99%

High‐Performance Liquid Chromatography of Biological Macromolecules

Irgum¹

2000

Encyclopedia of Analytical Chemistry

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Unusual Analyte Adsorption Effects on Inert Lc Components

Cited by 12 publications

References 3 publications

Characterization of low-molar-mass polymers by gradient polymer elution chromatography II. Solubility effects in the analysis of polyester resins under reversed-phase conditions

Characterization of low-molar-mass polymers by gradient polymer elution chromatography II. Solubility effects in the analysis of polyester resins under reversed-phase conditions

Differential adsorption of analyte and deuterated analogue onto the rotor seal of the injector in high‐performance liquid chromatography/electrospray ionisation mass spectrometry

High‐Performance Liquid Chromatography of Biological Macromolecules

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