Untwist and shout: a heavy metal-responsive transcriptional regulator

Summers, Anne O.

doi:10.1128/jb.174.10.3097-3101.1992

Cited by 215 publications

(180 citation statements)

References 43 publications

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“…The transcription start point is indicated by an asterisk, the -10 and -35 sequences are in bold letters, the protected bases are boxed and a DNase I hypersensitve bond is indicated with an arrow. merR mercury resistance operons in a wide variety of eubacteria (Summers, 1992). While TipAL-mediated transcriptional activation can presently best be understood in relation to this system, our data also revealed important features which are different from those observed for MerR and thus expand our understanding of this family of regulatory proteins.…”

Section: Resultsmentioning

confidence: 62%

“…In the merT and tipA promoters, the 19 bp spacing puts the presumed -35 and -10 hexamers out of phase with respect to the RNAP amino acid residues with which they must interact. MerR, upon binding Hg(ll), induces a 33 degree unwinding and thus allows functional alignment (Ansari et al, 1992 inducer (O'Halloran and Walsh, 1987) and activate transcription in combination with the ligand (Summers, 1992). This is unusual with respect to other prokaryotic transcriptional activators which interact with sequences within or upstream of the -35 hexamer and do not have an inactive promoter-bound form (Collado-Vides et al, 1991).…”

Section: Resultsmentioning

confidence: 99%

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Autogenous transcriptional activation of a thiostrepton-induced gene in Streptomyces lividans.

1993

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Although the antibiotic thiostrepton is best known as an inhibitor of protein synthesis, it also, at extremely low concentrations (< 10-9 M), induces the expression of a regulon of unknown function in certain Streptomyces species. Here, we report the purification of a Streptomyces lividans thiostrepton-induced transcriptional activator protein, TipAL, whose N-terminus is similar to a family of eubacterial regulatory proteins represented by MerR.TipAL was first purified from induced cultures of S.lividans as a factor which bound to and activated transcription from its own promoter. The tipAL gene was overexpressed in Escherichia coli and TipAL protein purifi'ed in a single step using a thiostrepton affinity column. Thiostrepton enhanced binding of TipAL to the promoter and catalysed specific transcription in vitro.TipAs, a second gene product of the same open reading frame consisting of the C-terminal domain of TipAL, is apparently translated using its own in-frame initiation site. Since it is produced in large molar excess relative to TipAL after induction and also binds thiostrepton, it may competitively modulate transcriptional activation.

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Section: Resultsmentioning

confidence: 62%

Section: Resultsmentioning

confidence: 99%

Autogenous transcriptional activation of a thiostrepton-induced gene in Streptomyces lividans.

1993

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“…The remaining two steps required for the formation of MBC are (i) oxidation of the hydroxymethyl group to an aldehyde and (ii) methylation of the hydroxyl group on ring B. PigF shows sequence identity to S-adenosylmethioninedependent methyltransferases, including RedI, and is assumed to be responsible for the methylation (Cerdeño et al, 2001;Summers et al, 1992). In Str.…”

Section: Biosynthesis Of Mbcmentioning

confidence: 99%

“…CueR is the transcriptional regulator of CopA. CueR is a member of the MerR family of transcriptional regulators, which occur in a range of bacterial genera and respond to a wide variety of external stimuli (Amábile-Cuevas & Demple, 1991;Brocklehurst et al, 1999;Chiu et al, 1999;Stoyanov et al, 2001;Summers, 1992). CopA is a copper-transporting P-type ATPase efflux pump and a central component of copper homeostasis in E. coli (Rensing et al, 2000).…”

Section: Relative Genomic Contexts Of the Pig Clustersmentioning

confidence: 99%

The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation

et al. 2004

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The prodigiosin biosynthesis gene cluster (pig cluster) from two strains of Serratia (S. marcescens ATCC 274 and Serratia sp. ATCC 39006) has been cloned, sequenced and expressed in heterologous hosts. Sequence analysis of the respective pig clusters revealed 14 ORFs in S. marcescens ATCC 274 and 15 ORFs in Serratia sp. ATCC 39006. In each Serratia species, predicted gene products showed similarity to polyketide synthases (PKSs), non-ribosomal peptide synthases (NRPSs) and the Red proteins of Streptomyces coelicolor A3(2). Comparisons between the two Serratia pig clusters and the red cluster from Str. coelicolor A3(2) revealed some important differences. A modified scheme for the biosynthesis of prodigiosin, based on the pathway recently suggested for the synthesis of undecylprodigiosin, is proposed. The distribution of the pig cluster within several Serratia sp. isolates is demonstrated and the presence of cryptic clusters in some strains shown. The pig cluster of Serratia marcescens ATCC 274 is flanked by cueR and copA homologues and this configuration is demonstrated in several S. marcescens strains, whilst these genes are contiguous in strains lacking the pig cluster.

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“…This result is consistent with the expectation that with a gapped spacer DNA, rotation of the -10 and the -35 regions with respect to each other can readily be accomplished without the introduction of further DNA distortion. i An additional indication of the importance of DNA untwisting was derived from study of the MerR system (Summers, 1992). The promoter directing transcription of the genes imparting resistance to Hg(ll) is poorly active, as expected in view of its 19bp spacer DNA.…”

Section: Promoters With Spacer Dna Modificationsmentioning

confidence: 99%

Open complex formation by Escherichia coli RNA polymerase: the mechanism of polymerase‐induced strand separation of double helical DNA

deHaseth

Helmann

1995

Molecular Microbiology

321

114

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SummaryEscherichia coli RNA polymerase is able to sitespecifically melt 12bp of promoter DNA at temperatures far below those normally associated with DNA melting. Here we consider several models to explain how RNA potymerase destabilizes duplex DNA. One popular model proposes that upon binding to the promoter, RNA polymerase untwists the spacer DNA between the 10 and 35 regions, which results in a destabilization of the 10 region at a TA base step where melting initiates. Promoter untwisting may result, in part, from extensive wrapping of the DNA around RNA polymerase. Formation of the strandseparated open complex appears to be facilitated by specific protein-DNA interactions which occur predominantly on the non-template strand. Recent evidence suggests that these include important contacts with Sigma factor region 2.3. which we propose binds the displaced single strand of DNA.

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Untwist and shout: a heavy metal-responsive transcriptional regulator

Cited by 215 publications

References 43 publications

Autogenous transcriptional activation of a thiostrepton-induced gene in Streptomyces lividans.

Autogenous transcriptional activation of a thiostrepton-induced gene in Streptomyces lividans.

The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation

Open complex formation by Escherichia coli RNA polymerase: the mechanism of polymerase‐induced strand separation of double helical DNA

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