Untrimmed ITS2 metabarcode sequences cause artificially reduced abundances of specific fungal taxa
Kathleen E. Kyle,
Jonathan L. Klassen
Abstract:Advances in DNA metabarcoding have greatly expanded our knowledge of microbial communities in recent years. Pipelines and parameters have been tested extensively for bacterial metabarcoding using the 16S rRNA gene and best practices are largely established. For fungal metabarcoding using the ITS gene, however, only a few studies have considered how such pipelines and parameters can affect community prediction. Here we report a novel bias uncovered during ITS2 sequencing ofTrichoderma-infected ant fungus garden… Show more
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