Untersuchungen zur unterschiedlichen Karzinominzidenz von Prostata und Samenblasen

Pannek, J.; Partin, A.

doi:10.1055/s-2002-32114

Cited by 3 publications

(2 citation statements)

References 14 publications

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“…Laser capture microdissection, however, allows for the selection of the pure cells of interest, as it is possible to microdissect the cells from frozen tissue sections using the LCM microscope. Some reports claim that LCM cell lysates of prostate cancer display specific protein patterns [27][28][29]. In one study, pure organ-matched cell populations were obtained using LCM from nine prostectomy specimens and were analyzed by SELDI MS [28].…”

Section: Prostate Cancermentioning

confidence: 99%

Revealing urologic diseases by proteomic techniques

Lee

Kim

2005

Journal of Chromatography B

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Section: Prostate Cancermentioning

confidence: 99%

Revealing urologic diseases by proteomic techniques

Lee

Kim

2005

Journal of Chromatography B

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“…A good example is prostate cancer, the most frequently diagnosed cancer and the second leading cause of cancer death in men in Western countries . The prostate marker PSA is quite sensitive, however, it does not correctly differentiate benign from malignant prostate disease, and can miss some significant prostate cancers. − It is warranted to search for additional biomarkers in order to improve cancer specificity. Most likely, multiple biomarkers will be required to improve early detection, diagnosis, and prognosis.…”

Section: Introductionmentioning

confidence: 99%

Derivatized Cellulose Combined with MALDI-TOF MS: A New Tool for Serum Protein Profiling

et al. 2005

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Providing a rapid and sensitive protein profiling method for biomarker discovery from a variety of biological samples is crucial for the introduction of new markers that improve cancer patient diagnosis at early tumor stages, thus increasing the chances of curative treatment. We report here the development and application of derivatized cellulose particles for selective serum protein profiling. For immobilized metal ion affinity chromatography (IMAC), cellulose was derivatized with glycidyl methacrylate (GMA) and subsequently with iminodiacetic acid (IDA). To investigate the application of this material for generating protein profiles of human serum samples, the serum samples were agitated with the derivatized cellulose particles to a suspension and incubated for 2 h at 30 degrees C. After washing, 1 microL of the IDA-Cu(2+)-cellulose suspension was applied directly onto a MALDI-target, mixed with sinapinic acid (SA) and analyzed with MALDI-TOF MS. Consistent serum specific data were obtained from aliquoted samples analyzed several times, indicating the reliability of the method. However, the serum fingerprints obtained proved to be specific for any given serum. The technique presented allows a high enrichment of sample on the developed target leading to a high sensitivity and reproducibility without depletion of albumin and immunoglobulin, and sample elution prior to MS-analysis. The study demonstrates for the first time that derivatized cellulose particles combined with MALDI-TOF MS represent a simple, economical, and rapid approach to generate serum protein profiles for biomarker identification.

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Primäres Adenokarzinom der Samenblasen

Möhring¹,

Bach²,

Kosciesza³

et al. 2008

Urologe

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Primary malignancies of the seminal vesicles are extremely rare. They must be strictly differentiated from surrounding malignancies that may infiltrate the seminal vesicles from outside (e.g. prostate, rectum, and bladder carcinoma). MEDLINE and CANCERLIT review showed about 50 documented cases of primary seminal vesicle carcinoma so far worldwide in men between 19 and 90 years of age. Early diagnosis may be difficult due to lack of specific symptoms. Especially with a history of voiding dysfunction, haemospermia and/or haematuria, investigators should consider it. Primary diagnostic steps include digital rectal examination, transrectal ultrasound, and transrectal biopsy of the tumour. Additionally, CT and MRT scans show tumour masses corresponding to the seminal vesicles. Adenocarcinoma of seminal vesicles shows no expression of prostate-specific antigen or prostate-specific acid phosphatase, but there may be expression of carcinoembryonic antigen and cancer antigen 125. Radical surgery including radical prostatectomy and/or cystoprostatectomy including pelvic lymph node dissection offers a curative treatment pathway. Adjuvant or inductive medical treatment is of unproven worth, but a combination of hormonal deprivation and radiotherapy seems to be more effective than any chemotherapy.

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Untersuchungen zur unterschiedlichen Karzinominzidenz von Prostata und Samenblasen

Cited by 3 publications

References 14 publications

Revealing urologic diseases by proteomic techniques

Revealing urologic diseases by proteomic techniques

Derivatized Cellulose Combined with MALDI-TOF MS: A New Tool for Serum Protein Profiling

Primäres Adenokarzinom der Samenblasen

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