Untersuchungen zur Struktur und Funktion des Lampenb�rsten-Y-Chromosoms in der Spermatogenese von Drosophila

Hennig, Wolfgang

doi:10.1007/bf00319879

Cited by 107 publications

(37 citation statements)

References 58 publications

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“…It has been earlier considered that the transcription in spermatogenesis after meiosis is very low or completely absent [5,14,15]. Barreau et al (2008) used RT-PCR and in situ hybridization to demonstrate that 96% of the genes whose mRNAs are detectable in spermatids are transcribed in spermatocytes [16].…”

Section: Discussionmentioning

confidence: 99%

GFP markers for studying D. melanogaster spermatogenesis

2009

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Localization of a set of chimeric GFP proteins in D. melanogaster spermatogenesis has been studied. In the collection used, the proteins with detectable germ-line expression frequently occur to be involved in mRNA maturation. According to the cellular localization, proteins fall into three groups, namely, the protein Rtc1, involved in splicing, displays an exclusively nuclear localization; the proteins Squid (splicing and mRNA transport), Pabp2 (polyadenylation), and Hrb98DE (splicing and mRNA transport) change their localization during germ-line development; and the protein Imp (mRNA localization) is located exclusively in the cytoplasm. The distribution of Rtc1, Squid and Hrb98DE in the spermatocyte nuclei is morphologically similar to the distribution of splicing bodies, the so-called splicing factor compartments. The proteins Imp and Hrb98DE at the stage of elongation are localized to a specialized structure in the caudal region of cyst; this region corresponds to the site of the highest synthetic activity in the cyst cells at this developmental stage.

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Section: Discussionmentioning

confidence: 99%

GFP markers for studying D. melanogaster spermatogenesis

2009

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“…in round spermatids Moss et al, 1994). In Drosophila, no transcription occurs after meiosis (Hennig, 1967), and the polyadenylated H2B mRNAs must be synthesized in spermatogonia or spermatocytes. It is possible, of course, that these transcripts are stored to be translated after meiosis.…”

Section: Utilization Of the Downstream Polyadenylation Signals Sim-mentioning

confidence: 99%

Two Types of Polyadenated mRNAs are Synthesized from Drosophila Replication‐Dependent Histone Genes

Akhmanova¹,

Miedema²,

Kremer³

et al. 1997

European Journal of Biochemistry

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The polyadenylation of replication-dependent histone H2B, H 3 and H4 mRNAs in Drosophilu melanoguster was analysed. Two types of mRNAs, containing a poly(A) tail, can be detected in addition to non-polyadenylated messengers, which represent the majority of replication-dependent histone mRNAs. Firstly, conventional polyadenylation signals, localized downstream from the stem-loop region, are used to produce polyadenylated mRNAs. The messengers of this type, generated from the D. melanogaster H2B gene, are preferentially synthesized in the testis of the fly. Secondly, a distinct type of polyadenylated histone mRNA has been identified. This mRNA, which is present in many different tissues and constitutes a minor part of the total histone mRNA pool, contains a short poly(A) tail, added to the end of the 3' terminal stem-loop structure, which is in most cases lacking several nucleotides from its 3' end. The sites of polyadenylation within the stem-loop are not preceded by a nornial polyadenylation signal. The possible functions of the polyadenylated histone transcripts are discussed.

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“…Approximately half the translocations involving the Y chromosome are also male sterile, but this sterility, in contrast to that caused by X-autosome translocations, is recessive. Unlike the X, the Y does engage in positive synthetic activity in primary spermatocytes (22,23), and impaired Y function, whether caused by changing the state of the chromosome or deleting specific fertility factors, may be complemented by the presence of an additional normal Y in the genome (24,25). We think of the activity of a chromosome as being under the control of factors on the same chromosome, i.e.…”

Section: Genetic Analysismentioning

confidence: 99%

The Role of X-Chromosome Inactivation during Spermatogenesis

Lifschytz

Lindsley

1972

Proc. Natl. Acad. Sci. U.S.A.

493

247

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Inactivation of the single X chromosome in the primary spermatocytes of species with heterogametic males is postulated as a basic control mechanism on the chromosomal level that is required for normal spermatogenesis. This view is supported by (a) cytological observations of X-chromosome allocycly in the primary spermatocytes of all male-heterogametic organisms that were adequately examined, (b) autoradiographic evidence of early cessation of transcription by the X chromosome in the mouse and three species of grasshopper, and (c) the male sterility of animals with certain X-chromosome rearrangements that cannot be attributed to misfunction of specific genes. X-chromosome inactivation during spermatogenesis is proposed as the ideal system for studies of genetic control at the chromosomal level.Sex chromosomes provide a striking example of differentiation of the chromosomal complement and inferentially of chromosomal function during evolution. It seems reasonable to suppose that natural selection has resulted in a particular distribution of genes between the sex chromosomes and the autosomes that is related to the role of these genes in the development and expression of sexuality. For example, malefertility genes are found on the Y chromosome (Y) in Drosophila, whereas male-determining genes are found on mammalian Y chromosomes. Furthermore, sexual differentiation might depend on males differing in constitution from females (e.g. dosage) with respect to some genes but not others; Bridges' and Goldschmidt's genic balance theories of sex determination (1,2) (11,12) have shown that early condensation of the X in mouse spermatocytes is correlated with late replication; furthermore, Monesi (13) correlated early condensation with genetic inactivation, using cessation of uridine incorporation as the criterion. Similar autoradiographic studies of several species of grasshopper by Henderson (14) demonstrated that the X chromosome replicates later in S (synthetic phase of the cell cycle) 182

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Untersuchungen zur Struktur und Funktion des Lampenb�rsten-Y-Chromosoms in der Spermatogenese von Drosophila

Cited by 107 publications

References 58 publications

GFP markers for studying D. melanogaster spermatogenesis

GFP markers for studying D. melanogaster spermatogenesis

Two Types of Polyadenated mRNAs are Synthesized from Drosophila Replication‐Dependent Histone Genes

The Role of X-Chromosome Inactivation during Spermatogenesis

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